Align Acetolactate synthase isozyme 2 large subunit; AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit; EC 2.2.1.6 (characterized)
to candidate WP_028583815.1 G494_RS0106080 acetolactate synthase, large subunit, biosynthetic type
Query= SwissProt::P0DP90 (548 letters) >NCBI__GCF_000429965.1:WP_028583815.1 Length = 560 Score = 484 bits (1246), Expect = e-141 Identities = 257/554 (46%), Positives = 351/554 (63%), Gaps = 14/554 (2%) Query: 1 MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGV-EHLLCRHEQGAAMAAIGYAR 59 + GA + L G+ T+ G PGGA +P+Y+AL V H+L RHEQGA A G AR Sbjct: 4 LTGATLTIRLLEHYGITTIAGIPGGANLPLYEALSQSSVIRHVLARHEQGAGFMAQGMAR 63 Query: 60 ATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSL 119 ++G+ VC ATSGPGATN++T +ADA LDSIPV+ ITGQV IGTDAFQEVD+ GL++ Sbjct: 64 SSGRPAVCFATSGPGATNILTAIADAYLDSIPVICITGQVPQDLIGTDAFQEVDIYGLTI 123 Query: 120 ACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKDIQLASGDLE--PWFTTVEN 177 TKH++LV+S EEL +++ EAF +A SGRPGPV++DIPKD+QL + + P + Sbjct: 124 PITKHNYLVRSGEELLKVIPEAFAIASSGRPGPVVIDIPKDVQLETVQVAGLPEIPALAA 183 Query: 178 EVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLAATKMPATCTLKGLGAV 237 V P + QA ++ +A+ P+LY+GGGV + A R +++P T TL GLG + Sbjct: 184 PVQVPAESLHQAASLINQAKAPVLYLGGGVVHSGAGLLARRLAERSQLPTTMTLMGLGII 243 Query: 238 EADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDP 297 ++ Y+GMLGMH ++ N + ECDLLIA G RFDDR TGK+ F P A +IH+DIDP Sbjct: 244 PPEHELYMGMLGMHAARSTNIMLDECDLLIAAGVRFDDRATGKIAEFCPDARIIHLDIDP 303 Query: 298 AEMNKLRQAHVALQGD----LNALLPAL---QQPLNQYDWQQHCAQLRDEHSWRYDHPGD 350 +E++KL+QAH+ L GD L LLP + ++PL WQQ QLR H + D Sbjct: 304 SEISKLKQAHLGLVGDIAQTLERLLPLVSRRERPL----WQQRVRQLRAAHPFFCAEELD 359 Query: 351 AIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGLGTMGFGLPAA 410 ++ ++++ +V TDVG+HQMW AQ RP F+TS GLGTMGFGLP A Sbjct: 360 FSRPYGIILRIAEIIGDQALVATDVGKHQMWTAQIYPLQRPRQFLTSGGLGTMGFGLPTA 419 Query: 411 VGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQ 470 +GA + P+ VVCISGDGS MMN+QEL T + L LK+V+L+NQ LG+VRQ Q+LF+ Sbjct: 420 IGAALQHPDKPVVCISGDGSIMMNIQELATAVEQDLNLKVVVLNNQSLGLVRQQQKLFYG 479 Query: 471 ERYSETTLTDNPDFLMLASAFGIHGQHITRKDQVEAALDTMLNSDGPYLLHVSIDELENV 530 R + NPD +A FG+ + + L L + GP L+++ ID E V Sbjct: 480 GRIFASEYRHNPDLAAIARGFGMKAFDCGQTLLFDEVLAVALQTPGPCLINIPIDIDEEV 539 Query: 531 WPLVPPGASNSEML 544 +P+VPPGA+NS M+ Sbjct: 540 YPMVPPGAANSVMI 553 Lambda K H 0.320 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 822 Number of extensions: 33 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 548 Length of database: 560 Length adjustment: 36 Effective length of query: 512 Effective length of database: 524 Effective search space: 268288 Effective search space used: 268288 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
Align candidate WP_028583815.1 G494_RS0106080 (acetolactate synthase, large subunit, biosynthetic type)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.12994.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.2e-204 665.4 0.0 3.6e-204 665.2 0.0 1.0 1 lcl|NCBI__GCF_000429965.1:WP_028583815.1 G494_RS0106080 acetolactate synt Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000429965.1:WP_028583815.1 G494_RS0106080 acetolactate synthase, large subunit, biosynthetic type # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 665.2 0.0 3.6e-204 3.6e-204 1 556 [. 4 555 .. 4 556 .. 0.98 Alignments for each domain: == domain 1 score: 665.2 bits; conditional E-value: 3.6e-204 TIGR00118 1 lkgaeilveslkkegvetvfGyPGGavlpiydaly.dselehilvrheqaaahaadGyarasGkvGvvl 68 l+ga++ ++ l++ g+ t+ G+PGGa lp+y+al +s ++h+l+rheq+a +a+G+ar+sG++ v++ lcl|NCBI__GCF_000429965.1:WP_028583815.1 4 LTGATLTIRLLEHYGITTIAGIPGGANLPLYEALSqSSVIRHVLARHEQGAGFMAQGMARSSGRPAVCF 72 689*******************************978889***************************** PP TIGR00118 69 atsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpei 137 atsGPGatn++t+ia+aylds+P++ +tGqv+++liG+dafqe+di G+t+p+tkh++lv++ e+l ++ lcl|NCBI__GCF_000429965.1:WP_028583815.1 73 ATSGPGATNILTAIADAYLDSIPVICITGQVPQDLIGTDAFQEVDIYGLTIPITKHNYLVRSGEELLKV 141 ********************************************************************* PP TIGR00118 138 lkeafeiastGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvll 206 + eaf ias+GrPGPv++d+Pkdv+ +++++ e+p+ + v+ +++a+ li++ak Pvl+ lcl|NCBI__GCF_000429965.1:WP_028583815.1 142 IPEAFAIASSGRPGPVVIDIPKDVQLETVQVAGL--PEIPALAAPVQVPAESLHQAASLINQAKAPVLY 208 **************************99988766..689999999999999****************** PP TIGR00118 207 vGgGviiaeaseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliavGa 275 GgGv++++a ++laer ++p t+tl+GlG +p +h+l +gmlGmh ++ +n+ ++e+dllia G+ lcl|NCBI__GCF_000429965.1:WP_028583815.1 209 LGGGVVHSGAGLLARRLAERSQLPTTMTLMGLGIIPPEHELYMGMLGMHAARSTNIMLDECDLLIAAGV 277 ********************************************************************* PP TIGR00118 276 rfddrvtgnlakfapeakiihididPaeigknvkvdipivGdakkvleellkklkeeekkekeWlekie 344 rfddr tg++a+f+p+a+iih+didP+ei+k ++++ +vGd + le+ll ++++e+ W ++++ lcl|NCBI__GCF_000429965.1:WP_028583815.1 278 RFDDRATGKIAEFCPDARIIHLDIDPSEISKLKQAHLGLVGDIAQTLERLLPLVSRRERPL--WQQRVR 344 *****************************************************99988777..****** PP TIGR00118 345 ewkkeyilkldeeeesikPqkvikelskllkdeaivttdvGqhqmwaaqfyktkkprkfitsgGlGtmG 413 ++++ +++ ee + +P +i ++ +++ d+a+v+tdvG+hqmw+aq y+ ++pr+f+tsgGlGtmG lcl|NCBI__GCF_000429965.1:WP_028583815.1 345 QLRAAHPFFCAEELDFSRPYGIILRIAEIIGDQALVATDVGKHQMWTAQIYPLQRPRQFLTSGGLGTMG 413 ********************************************************************* PP TIGR00118 414 fGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWqelfyeery 482 fGlP+a+Ga + +p++ vv+++Gdgs++mn+qel+t+ve d+++k+v+lnn+ lG+v+q q+lfy +r lcl|NCBI__GCF_000429965.1:WP_028583815.1 414 FGLPTAIGAALQHPDKPVVCISGDGSIMMNIQELATAVEQDLNLKVVVLNNQSLGLVRQQQKLFYGGRI 482 ********************************************************************* PP TIGR00118 483 setklaselpdfvklaeayGvkgiriekpeeleeklkealeskepvlldvevdkeeevlPmvapGagld 551 +++ ++pd++++a+++G+k+ + +e l+ al++ +p l+++ +d +eev+Pmv+pGa+++ lcl|NCBI__GCF_000429965.1:WP_028583815.1 483 FASEYR-HNPDLAAIARGFGMKAFDCGQTLLFDEVLAVALQTPGPCLINIPIDIDEEVYPMVPPGAANS 550 *****9.59***********************************************************9 PP TIGR00118 552 elvee 556 +++e lcl|NCBI__GCF_000429965.1:WP_028583815.1 551 VMIGE 555 99976 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (560 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 10.81 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory