Align dihydroxy-acid dehydratase subunit (EC 4.2.1.9) (characterized)
to candidate WP_027178267.1 G496_RS0104725 dihydroxy-acid dehydratase
Query= metacyc::MONOMER-11919 (549 letters) >NCBI__GCF_000429985.1:WP_027178267.1 Length = 555 Score = 568 bits (1465), Expect = e-166 Identities = 279/556 (50%), Positives = 401/556 (72%), Gaps = 9/556 (1%) Query: 1 MKSDTIKRGIQRAPHRSLLARCGLTDDDFEKPFIGIANSYTDIVPGHIHLRELAEAVKEG 60 M+S + G+++APHRSLL G+ ++ ++P IG+ N+ +I+PGH+HL + +AVK+G Sbjct: 1 MRSKKMTGGLEKAPHRSLLYALGMCKEEMDRPLIGVCNAANEIIPGHMHLHNIVKAVKDG 60 Query: 61 VNAAGGVAFEFNTMAICDGIAMNHDGMKYSLASREIVADTVESMAMAHALDGLVLLPTCD 120 V GGV EF + +CDG+AMNH+GMK+SL SRE++AD++E MA AH D LVL+P CD Sbjct: 61 VRLGGGVPMEFPAIGVCDGLAMNHEGMKFSLPSREVIADSIEIMATAHPFDALVLVPNCD 120 Query: 121 KIVPGMLMAAARLDIPAIVVTGGPMLPGEFKGRKVDLINVYEGVGTVSAGEMSEDELEEL 180 KIVPGMLMAA R++IP ++V+GG MLPG G+KVDLI V+EGVG V G M+EDEL L Sbjct: 121 KIVPGMLMAALRINIPTVIVSGGAMLPGSKNGKKVDLITVFEGVGMVKTGNMTEDELSGL 180 Query: 181 ERCACPGPRSCAGLFTANTMACLTEALGMSLPGCATAHAVSSRKRQIARLSGKRIVEMVQ 240 E+ ACP SC+G+FTAN+M CL+E +G++LPG T AV S + ++A+ +G +++E+++ Sbjct: 181 EQSACPTCGSCSGMFTANSMNCLSETIGLALPGNGTIPAVMSDRIRLAKNAGLQVMEVLK 240 Query: 241 ENLKPTMIMSQEAFENAVMVDLALGGSTNTTLHIPAIAAEIDGLNINLDLFDELSRVIPH 300 +++KP I+++++ +NAV +D+ALG STNT LH+PAI E G+ ++L +FDE+SR P+ Sbjct: 241 KDIKPRDIVTEKSLKNAVTMDMALGCSTNTVLHLPAIFNEA-GIKLDLSIFDEISRKTPN 299 Query: 301 IASISPAGEHMMLDLDRAGGIPAVLKTL--EDHINRECVTCTGRTVQENIENVKVG--HR 356 + +SPAG H + +L+ AGGIP V+ L E + + +T TG+T+ EN++++ G + Sbjct: 300 LCKLSPAGPHYISELNEAGGIPGVMAELAKEGRLELDAITVTGKTIGENLKDLNAGITNH 359 Query: 357 DVIRPLDSPVHSEGGLAILRGNLAPRGSVVKQGAVAEDMMVHEGPAKVFNSEDECMEAIF 416 ++++P+D P EGG+A+L GN+A G VVKQ AVA +MM G A+VFNSE+E +EAI Sbjct: 360 EIVKPIDKPYSKEGGIAVLYGNIAEEGCVVKQSAVAPEMMHRTGVAQVFNSEEESVEAIL 419 Query: 417 GGRIDEGDVIVIRYEGPKGGPGMREMLNPTSAIAGMGL-ERVALITDGRFSGGTRGPCVG 475 GG+I +GDV+VI YEGPKGGPGMREML PTSAI+GMGL VALITDGRFSGGTRG +G Sbjct: 420 GGKIKKGDVVVILYEGPKGGPGMREMLTPTSAISGMGLGSDVALITDGRFSGGTRGAAIG 479 Query: 476 HVSPEAMEDGPLAAVNDGDIIRIDIPSRKLEVDLSPREIEERLQSAVKP--RRSVKGWLA 533 H+SPEA G + + GD I I+IP R + + + E+++R ++ KP + +L Sbjct: 480 HISPEAASGGTVGLIKTGDKIEINIPERTINLLVDDAELKKR-RADFKPIKKEVTSSFLK 538 Query: 534 RYRKLAGSADTGAVLR 549 RY + SA TGAV + Sbjct: 539 RYSENVTSASTGAVFK 554 Lambda K H 0.319 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 981 Number of extensions: 45 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 549 Length of database: 555 Length adjustment: 36 Effective length of query: 513 Effective length of database: 519 Effective search space: 266247 Effective search space used: 266247 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate WP_027178267.1 G496_RS0104725 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.20863.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6.5e-241 786.5 10.9 7.4e-241 786.3 10.9 1.0 1 lcl|NCBI__GCF_000429985.1:WP_027178267.1 G496_RS0104725 dihydroxy-acid de Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000429985.1:WP_027178267.1 G496_RS0104725 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 786.3 10.9 7.4e-241 7.4e-241 1 542 [. 14 554 .. 14 555 .] 1.00 Alignments for each domain: == domain 1 score: 786.3 bits; conditional E-value: 7.4e-241 TIGR00110 1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 ++r+ll+a+G+ +e++++P+i+v+n+ +ei+Pgh+hl+++ k+vk++++ Ggv++ef i+v+DG+am lcl|NCBI__GCF_000429985.1:WP_027178267.1 14 PHRSLLYALGMCKEEMDRPLIGVCNAANEIIPGHMHLHNIVKAVKDGVRLGGGVPMEFPAIGVCDGLAM 82 69******************************************************************* PP TIGR00110 70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138 +heGmk+sLpsre+iaDs+e +++ah +Dalv++++CDkivPGmlmaalr+niP+++vsGG+m +g + lcl|NCBI__GCF_000429985.1:WP_027178267.1 83 NHEGMKFSLPSREVIADSIEIMATAHPFDALVLVPNCDKIVPGMLMAALRINIPTVIVSGGAMLPGSKN 151 ******************************************************************999 PP TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207 ++k+dl+ vfe+vg+++ g+++e+el+ +e+sacPt+gsCsG+ftansm+cl+e++Gl+lPg++t++a lcl|NCBI__GCF_000429985.1:WP_027178267.1 152 -GKKVDLITVFEGVGMVKTGNMTEDELSGLEQSACPTCGSCSGMFTANSMNCLSETIGLALPGNGTIPA 219 .9******************************************************************* PP TIGR00110 208 tsaekkelakksgkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklsld 276 + +++ +lak++g +++e++kk+ikPrdi+t+++++na+t+d+alG stntvLhl+ai +eag+kl+l+ lcl|NCBI__GCF_000429985.1:WP_027178267.1 220 VMSDRIRLAKNAGLQVMEVLKKDIKPRDIVTEKSLKNAVTMDMALGCSTNTVLHLPAIFNEAGIKLDLS 288 ********************************************************************* PP TIGR00110 277 dfdrlsrkvPllaklkPsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlrv 345 fd++srk+P l+kl+P+g ++i +l++aGG+++v+ el keg l+ da+tvtGkt++e+l++ + + lcl|NCBI__GCF_000429985.1:WP_027178267.1 289 IFDEISRKTPNLCKLSPAGPHYISELNEAGGIPGVMAELAKEGRLELDAITVTGKTIGENLKDLNAGIT 357 ********************************************************************* PP TIGR00110 346 dqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvkeG 414 ++++++++d+p++kegg+avL+Gn+aeeG+vvk+++v+ ++++ +G a+vf+seee +eailggk+k+G lcl|NCBI__GCF_000429985.1:WP_027178267.1 358 NHEIVKPIDKPYSKEGGIAVLYGNIAEEGCVVKQSAVAPEMMHRTGVAQVFNSEEESVEAILGGKIKKG 426 ********************************************************************* PP TIGR00110 415 dvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialve 483 dvvvi yeGPkGgPGmremL+Ptsa++g+GLg++vaLitDGrfsGgtrG +iGh+sPeaa+gG+++l++ lcl|NCBI__GCF_000429985.1:WP_027178267.1 427 DVVVILYEGPKGGPGMREMLTPTSAISGMGLGSDVALITDGRFSGGTRGAAIGHISPEAASGGTVGLIK 495 ********************************************************************* PP TIGR00110 484 dGDkikiDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavld 542 GDki+i+i++r+++l v+++el++rra+ k+ +++ ++ +L++y++ v+sa++Gav + lcl|NCBI__GCF_000429985.1:WP_027178267.1 496 TGDKIEINIPERTINLLVDDAELKKRRADFKPIKKEVTSSFLKRYSENVTSASTGAVFK 554 **********************************99*********************86 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (555 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.02s 00:00:00.05 Elapsed: 00:00:00.03 # Mc/sec: 7.60 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory