Alignments for a candidate for aroG in Azohydromonas australica DSM 1124

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroG in Azohydromonas australica DSM 1124

Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate WP_029001555.1 H537_RS0134720 3-deoxy-7-phosphoheptulonate synthase

Query= BRENDA::C3TIE2
         (350 letters)



>NCBI__GCF_000430725.1:WP_029001555.1
          Length = 375

 Score =  428 bits (1100), Expect = e-124
 Identities = 217/352 (61%), Positives = 258/352 (73%), Gaps = 5/352 (1%)

Query: 4   QNDDLRIKEIKELLPPVALLEKFPATENAANT-VAHARKAIHKILKGNDDRLLVVIGPCS 62
           Q DD RI++I  L PP  L+  FP         V   R+ I +IL G DDRLLV++GPCS
Sbjct: 24  QTDDARIEDITPLPPPEHLIRFFPIRNTPVEALVTQTRQRIGQILHGQDDRLLVIMGPCS 83

Query: 63  IHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTVGWKGLINDPHMDNSFQINDG 122
           IHDP AA EYA RL+  R    D LE+VMRVYFEKPRTTVGWKGLINDP++D SF+I++G
Sbjct: 84  IHDPAAALEYARRLMEQRRRYADTLEVVMRVYFEKPRTTVGWKGLINDPYLDESFRIDEG 143

Query: 123 LRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWGAIGARTTESQVHRELASGLSCP 182
           LR AR+LL++IN  GLPAA EFLD+I+PQY+ DL++WGAIGARTTESQVHRELASG+S P
Sbjct: 144 LRFARQLLVEINRLGLPAASEFLDVISPQYIGDLIAWGAIGARTTESQVHRELASGISAP 203

Query: 183 VGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHSAIVNTSGNGDCHIILRGGKEPNYS 242
           +GFKNGTDG IK+AIDAI AA  PH FLSV K G  A V T GN DCH+ILRGGK PNY 
Sbjct: 204 IGFKNGTDGNIKIAIDAIQAASRPHHFLSVHKNGQVATVQTKGNPDCHVILRGGKAPNYD 263

Query: 243 AKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFKKQMDVCADVCQQIAGGEKAIIGVMVE 302
           A HVA     L  AGL   +M+DFSHANSSK+ ++Q+DV  DV  QI  G ++I GVMVE
Sbjct: 264 ADHVAAACRDLQAAGLDTSLMVDFSHANSSKKHERQVDVARDVAAQIRSGGRSIFGVMVE 323

Query: 303 SHLVEGNQSLESGE----PLAYGKSITDACIGWEDTDALLRQLANAVKARRG 350
           SHL  G Q    G+     L YG+SITDACIGW+D+  +L +L+ AVKARRG
Sbjct: 324 SHLQPGAQKFTPGKDDASKLVYGQSITDACIGWDDSLTVLEELSAAVKARRG 375


Lambda     K      H
   0.317    0.134    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 403
Number of extensions: 8
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 350
Length of database: 375
Length adjustment: 29
Effective length of query: 321
Effective length of database: 346
Effective search space:   111066
Effective search space used:   111066
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate WP_029001555.1 H537_RS0134720 (3-deoxy-7-phosphoheptulonate synthase)
to HMM TIGR00034 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00034.hmm
# target sequence database:        /tmp/gapView.9143.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00034  [M=342]
Accession:   TIGR00034
Description: aroFGH: 3-deoxy-7-phosphoheptulonate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   4.4e-165  534.4   0.0   5.1e-165  534.2   0.0    1.0  1  lcl|NCBI__GCF_000430725.1:WP_029001555.1  H537_RS0134720 3-deoxy-7-phospho


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000430725.1:WP_029001555.1  H537_RS0134720 3-deoxy-7-phosphoheptulonate synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  534.2   0.0  5.1e-165  5.1e-165       1     342 []      26     374 ..      26     374 .. 0.97

  Alignments for each domain:
  == domain 1  score: 534.2 bits;  conditional E-value: 5.1e-165
                                 TIGR00034   1 ddlrivkidelltPeelkakfpltek.aaekvaksrkeiadilaGkddrllvviGPcsihdpeaaleya 68 
                                               dd ri++i +l +Pe+l++ fp+ ++ +++ v+++r++i +il+G+ddrllv++GPcsihdp aaleya
  lcl|NCBI__GCF_000430725.1:WP_029001555.1  26 DDARIEDITPLPPPEHLIRFFPIRNTpVEALVTQTRQRIGQILHGQDDRLLVIMGPCSIHDPAAALEYA 94 
                                               799********************987256789************************************* PP

                                 TIGR00034  69 krlkklaeklkddleivmrvyfekPrttvGWkGlindPdlnesfdvnkGlriarkllldlvelglplat 137
                                               +rl + + ++ d le+vmrvyfekPrttvGWkGlindP+l+esf++++Glr ar+ll++++ lglp+a+
  lcl|NCBI__GCF_000430725.1:WP_029001555.1  95 RRLMEQRRRYADTLEVVMRVYFEKPRTTVGWKGLINDPYLDESFRIDEGLRFARQLLVEINRLGLPAAS 163
                                               ********************************************************************* PP

                                 TIGR00034 138 elldtispqyladllswgaiGarttesqvhrelasglslpvgfkngtdGslkvaidairaaaaehlfls 206
                                               e+ld+ispqy++dl++wgaiGarttesqvhrelasg+s p+gfkngtdG++k+aidai+aa+++h+fls
  lcl|NCBI__GCF_000430725.1:WP_029001555.1 164 EFLDVISPQYIGDLIAWGAIGARTTESQVHRELASGISAPIGFKNGTDGNIKIAIDAIQAASRPHHFLS 232
                                               ********************************************************************* PP

                                 TIGR00034 207 vtkaGqvaivetkGnedghiilrGGkkpnydaedvaevkeelekaglkeelmidfshgnsnkdykrqle 275
                                               v+k+Gqva v+tkGn+d+h+ilrGGk+pnyda++va+++++l++agl  +lm+dfsh+ns+k+++rq++
  lcl|NCBI__GCF_000430725.1:WP_029001555.1 233 VHKNGQVATVQTKGNPDCHVILRGGKAPNYDADHVAAACRDLQAAGLDTSLMVDFSHANSSKKHERQVD 301
                                               ********************************************************************* PP

                                 TIGR00034 276 vaesvveqiaeGekaiiGvmiesnleeGnqsl...k...eelkyGksvtdacigwedteallrklaeav 338
                                               va++v++qi++G ++i+Gvm+es+l+ G+q+    k   ++l+yG+s+tdacigw+d+ ++l++l++av
  lcl|NCBI__GCF_000430725.1:WP_029001555.1 302 VARDVAAQIRSGGRSIFGVMVESHLQPGAQKFtpgKddaSKLVYGQSITDACIGWDDSLTVLEELSAAV 370
                                               ******************************974431333679*************************** PP

                                 TIGR00034 339 kerr 342
                                               k+rr
  lcl|NCBI__GCF_000430725.1:WP_029001555.1 371 KARR 374
                                               *996 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (342 nodes)
Target sequences:                          1  (375 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 7.26
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis