Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (characterized)
to candidate WP_028486148.1 B076_RS0103865 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA
Query= SwissProt::O06491 (485 letters) >NCBI__GCF_000483485.1:WP_028486148.1 Length = 484 Score = 493 bits (1268), Expect = e-144 Identities = 244/476 (51%), Positives = 330/476 (69%), Gaps = 1/476 (0%) Query: 8 ITELKQLIHKKEIKISDLVDESYKRIQAVDDKVQAFLALDEERARAYAKELDEAVDGRSE 67 I ++ + +H EI L RI D ++ A++++ E A A++ D+ + E Sbjct: 6 IKQMSEKLHAGEITSVQLTQHYLDRISKYDTELNAYVSVTPELAMMMAEQADKKL-ASGE 64 Query: 68 HGLLFGMPIGVKDNIVTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIGKLNMDEF 127 LL G+P+ KD T G++T+CSSK+L+NF YDA VV +L+ +GK NMDEF Sbjct: 65 ADLLTGIPVAHKDIFCTDGVKTSCSSKMLDNFIAPYDAHVVSQLKKVGMPILGKTNMDEF 124 Query: 128 AMGSSTENSAYKLTKNPWNLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIRQPASFCG 187 AMGSS+E+S Y TKNPWN VPGGSSGG+AA +AAG P + G+DTGGSIRQPASFCG Sbjct: 125 AMGSSSESSYYGPTKNPWNHQAVPGGSSGGAAAVIAAGLAPMATGTDTGGSIRQPASFCG 184 Query: 188 VVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTSANVDVPD 247 + G+KPTYG VSR+G+VA+ASS DQ GP+TR+ +D+A+LL A++G D+ DSTS + D Sbjct: 185 ITGIKPTYGSVSRFGIVAYASSFDQAGPMTRSAQDSAWLLNAMAGFDERDSTSLQREAVD 244 Query: 248 FLSSLTGDIKGLKIAVPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLPHSKYALA 307 + + L +KGLK+ VP EY GEG+ + V A+ +E LGA EV LP+ A+ Sbjct: 245 YAAELGQSLKGLKVGVPAEYFGEGLDADVEAVVRDAIAEVEKLGAEVVEVHLPNKDLAVP 304 Query: 308 TYYLLSSSEASANLARFDGIRYGYRTDNADNLIDLYKQTRAEGFGNEVKRRIMLGTFALS 367 +YY+L+ +EAS+NL+RFDG+R+GYR +N +L DLYK++R+EG G EVKRRIM+G +ALS Sbjct: 305 SYYVLAPAEASSNLSRFDGVRFGYRCENPKDLEDLYKRSRSEGLGAEVKRRIMVGAYALS 364 Query: 368 SGYYDAYYKKAQKVRTLIKKDFEDVFEKYDVIVGPTTPTPAFKIGENTKDPLTMYANDIL 427 +GYYDAYY KAQK+R +++ DF FE DVI+GP PTPAF IGE T DP +MY D+ Sbjct: 365 AGYYDAYYLKAQKLRRMVRDDFTKAFESCDVIMGPVAPTPAFNIGEKTDDPTSMYLADLY 424 Query: 428 TIPVNLAGVPGISVPCGLADGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHKAKP 483 TIPVNLAG+PGISVP G + P+GL I+G +F E+ + VAH ++Q TD H P Sbjct: 425 TIPVNLAGLPGISVPAGFVNDRPVGLHIVGPYFSETKLLNVAHQYQQVTDWHTRMP 480 Lambda K H 0.315 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 605 Number of extensions: 24 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 485 Length of database: 484 Length adjustment: 34 Effective length of query: 451 Effective length of database: 450 Effective search space: 202950 Effective search space used: 202950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
Align candidate WP_028486148.1 B076_RS0103865 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA)
to HMM TIGR00132 (gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00132.hmm # target sequence database: /tmp/gapView.7496.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00132 [M=466] Accession: TIGR00132 Description: gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.7e-191 620.8 0.0 9e-191 620.5 0.0 1.0 1 lcl|NCBI__GCF_000483485.1:WP_028486148.1 B076_RS0103865 Asp-tRNA(Asn)/Glu Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000483485.1:WP_028486148.1 B076_RS0103865 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 620.5 0.0 9e-191 9e-191 3 465 .. 11 474 .. 9 475 .. 0.99 Alignments for each domain: == domain 1 score: 620.5 bits; conditional E-value: 9e-191 TIGR00132 3 ellkkkevsikevleeilerieavkdkinaflevtkekalkkakkldkkva.ke.kklagipiavKdni 69 e+l++ e++++++++++l+ri++ + ++na+++vt+e a+ +a++ dkk+a e ++l+gip+a Kd + lcl|NCBI__GCF_000483485.1:WP_028486148.1 11 EKLHAGEITSVQLTQHYLDRISKYDTELNAYVSVTPELAMMMAEQADKKLAsGEaDLLTGIPVAHKDIF 79 6789999*******************************************985647************* PP TIGR00132 70 avkdiettcaSkiLenyvspydatVverlkeagaliiGktNlDEFamGsstetSafgvtknPeneervp 138 ++ +++t+c+Sk+L+n+++pyda+Vv +lk+ g+ i+GktN+DEFamGss+e+S++g+tknP+n++ vp lcl|NCBI__GCF_000483485.1:WP_028486148.1 80 CTDGVKTSCSSKMLDNFIAPYDAHVVSQLKKVGMPILGKTNMDEFAMGSSSESSYYGPTKNPWNHQAVP 148 ********************************************************************* PP TIGR00132 139 GGSsgGsaaavaadlvplalgsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiGilakkv 207 GGSsgG+aa++aa+l+p+a g+DTGgSiRqPAsfcg+ G+KPtYG vSR+G+vayasS+dq G+++++ lcl|NCBI__GCF_000483485.1:WP_028486148.1 149 GGSSGGAAAVIAAGLAPMATGTDTGGSIRQPASFCGITGIKPTYGSVSRFGIVAYASSFDQAGPMTRSA 217 ********************************************************************* PP TIGR00132 208 edialvldvisgkDkkDstslevkveelleelkkdlkglkvgvvkelseesldkevkekfeklleklee 276 +d a +l++++g D++Dstsl+ + ++ el + lkglkvgv e+++e+ld +v++ +++++ ++e+ lcl|NCBI__GCF_000483485.1:WP_028486148.1 218 QDSAWLLNAMAGFDERDSTSLQREAVDYAAELGQSLKGLKVGVPAEYFGEGLDADVEAVVRDAIAEVEK 286 ********************************************************************* PP TIGR00132 277 lgaeivevslpsvklalaiYyiispsEassnlarydgiryGkrveelkslkelyaktRsegfgeevkrR 345 lgae+vev+lp+ +la++ Yy+++p+Eassnl+r+dg+r+G+r e++k+l++ly+++Rseg+g+evkrR lcl|NCBI__GCF_000483485.1:WP_028486148.1 287 LGAEVVEVHLPNKDLAVPSYYVLAPAEASSNLSRFDGVRFGYRCENPKDLEDLYKRSRSEGLGAEVKRR 355 ********************************************************************* PP TIGR00132 346 imlGayalskeyydkyykkAqkvrtliidefeklfeevDvivsptaptlafklgekaedplemylsDvl 414 im+Gayals++yyd+yy+kAqk+r++++d+f+k+fe++Dvi++p+apt af++gek++dp +myl+D++ lcl|NCBI__GCF_000483485.1:WP_028486148.1 356 IMVGAYALSAGYYDAYYLKAQKLRRMVRDDFTKAFESCDVIMGPVAPTPAFNIGEKTDDPTSMYLADLY 424 ********************************************************************* PP TIGR00132 415 tvpanlaGlpaisvPlgkkekglpiGlqiigkafddkkllsvakaleqald 465 t+p+nlaGlp+isvP+g ++ p+Gl i+g +f+++kll+va++++q +d lcl|NCBI__GCF_000483485.1:WP_028486148.1 425 TIPVNLAGLPGISVPAGFV-NDRPVGLHIVGPYFSETKLLNVAHQYQQVTD 474 *******************.7*************************99876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (466 nodes) Target sequences: 1 (484 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 11.50 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory