Align aspartate kinase (EC 2.7.2.4) (characterized)
to candidate WP_028486187.1 B076_RS0104080 aspartate kinase
Query= BRENDA::O69077 (412 letters) >NCBI__GCF_000483485.1:WP_028486187.1 Length = 408 Score = 545 bits (1404), Expect = e-160 Identities = 282/407 (69%), Positives = 336/407 (82%), Gaps = 2/407 (0%) Query: 1 MALIVQKFGGTSVGTVERIEQVAEKVKKFREAGDDVVVVVSAMSGETNRLIGLANQIMEQ 60 MALIVQK+GGTSVG +ERI+ VA KV KF + G VVV VSAMSGETNRL +A++I + Sbjct: 1 MALIVQKYGGTSVGNIERIQNVANKVAKFVDDGHQVVVAVSAMSGETNRLTAMAHEIQAR 60 Query: 61 PVPRELDVMVSTGEQVTIALLSMALIKRGVPAVSYTGNQVRILTDSAHTKARILHIDDTH 120 P+ RE+DV+++TGEQVTIALLSMAL ++G PA+SYTG QV I TD+ H+KARI ID Sbjct: 61 PIKREMDVLLTTGEQVTIALLSMALQEKGYPAISYTGWQVPIKTDAVHSKARIEEIDSEK 120 Query: 121 IRADLKAGRVVVVAGFQGVDGNGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT 180 I L G+VVVVAGFQGV +G+ITTLGRGGSDTT VALAAAL ADECQIYTDVDGVYT Sbjct: 121 IFGQLNQGKVVVVAGFQGVTPDGDITTLGRGGSDTTAVALAAALNADECQIYTDVDGVYT 180 Query: 181 TDPRVVPQARRLDKITFEEMLEMASLGSKVLQIRAVEFAGKYNVPLRVLHSFQEGPGTLI 240 TDPRVVPQA+RLD ITFEEMLEMASLG+KVLQIR+VEFA KYNVPLRVL S Q+G GTLI Sbjct: 181 TDPRVVPQAKRLDHITFEEMLEMASLGAKVLQIRSVEFASKYNVPLRVLSSLQDGGGTLI 240 Query: 241 TIDD--EEESMEQPIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISAANVEVDMIVQN 298 T ++ +E ME+P+ISGIAF+RDEAKL I GVPD PGVA++ILGPIS AN+E+DMI+QN Sbjct: 241 TSEENFKENEMEKPLISGIAFSRDEAKLMILGVPDKPGVAYQILGPISDANIEIDMIIQN 300 Query: 299 VAHDNTTDFTFTVHRNDYLNALEILKQTAANIGAREAIGDTNIAKVSIVGVGMRSHAGVA 358 D TTDFTFT+ R D AL++LK TA +GARE + D I K+S+VGVGM+SH+G+A Sbjct: 301 QGIDGTTDFTFTIARGDLPVALDLLKSTANKLGAREVLSDDTIVKISMVGVGMKSHSGIA 360 Query: 359 SRMFEALAKESINIQMISTSEIKVSVVIEEKYLELAVRALHTAFELD 405 S+MF+ LA INIQMI T+EIK+SVVI+E+YLELAV++LH AFELD Sbjct: 361 SQMFKTLADNDINIQMIGTTEIKISVVIDEQYLELAVQSLHEAFELD 407 Lambda K H 0.317 0.133 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 515 Number of extensions: 16 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 412 Length of database: 408 Length adjustment: 31 Effective length of query: 381 Effective length of database: 377 Effective search space: 143637 Effective search space used: 143637 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_028486187.1 B076_RS0104080 (aspartate kinase)
to HMM TIGR00656 (aspartate kinase, monofunctional class (EC 2.7.2.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00656.hmm # target sequence database: /tmp/gapView.14464.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00656 [M=407] Accession: TIGR00656 Description: asp_kin_monofn: aspartate kinase, monofunctional class Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.4e-138 445.8 8.5 8.3e-138 445.7 8.5 1.0 1 lcl|NCBI__GCF_000483485.1:WP_028486187.1 B076_RS0104080 aspartate kinase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000483485.1:WP_028486187.1 B076_RS0104080 aspartate kinase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 445.7 8.5 8.3e-138 8.3e-138 1 406 [. 1 406 [. 1 407 [. 0.98 Alignments for each domain: == domain 1 score: 445.7 bits; conditional E-value: 8.3e-138 TIGR00656 1 veliVqKFGGtsvgsserikkaakivlkelkegkkvvVVvSAmskvtdelvelaellklleaisdeisp 69 + liVqK+GGtsvg++eri+++a++v k + +g++vvV vSAms++t++l ++a + i+ + + lcl|NCBI__GCF_000483485.1:WP_028486187.1 1 MALIVQKYGGTSVGNIERIQNVANKVAKFVDDGHQVVVAVSAMSGETNRLTAMA------HEIQARPIK 63 579***************************************************......899****** PP TIGR00656 70 rerdelvsvGEllssallssalrelgvkaealdgkeagilTddefgnAkikelateerLlelLeegiiv 138 re+d l+ +GE++++alls+al+e+g+ a++++g++ +i Td+ + +A+i+e++ e+++ +L++g++v lcl|NCBI__GCF_000483485.1:WP_028486187.1 64 REMDVLLTTGEQVTIALLSMALQEKGYPAISYTGWQVPIKTDAVHSKARIEEIDS-EKIFGQLNQGKVV 131 *******************************************************.************* PP TIGR00656 139 vvaGFiGateeGeiTtLGRGGSDltAallaaalkAdrveiyTDVeGvyttDPrvveeakkidkisyeEa 207 vvaGF+G t +G+iTtLGRGGSD+tA++laaal+Ad+++iyTDV+GvyttDPrvv++ak++d+i++eE+ lcl|NCBI__GCF_000483485.1:WP_028486187.1 132 VVAGFQGVTPDGDITTLGRGGSDTTAVALAAALNADECQIYTDVDGVYTTDPRVVPQAKRLDHITFEEM 200 ********************************************************************* PP TIGR00656 208 lelAtlGakvlhpralelaveakvpilvrsskekeegTlitn.......kkensslvkaialeknvarl 269 le+A+lGakvl+ r++e+a +++vp++v ss + gTlit+ ++e++ l+++ia++++ a+l lcl|NCBI__GCF_000483485.1:WP_028486187.1 201 LEMASLGAKVLQIRSVEFASKYNVPLRVLSSLQDGGGTLITSeenfkenEMEKP-LISGIAFSRDEAKL 268 *****************************************9999987666666.************** PP TIGR00656 270 tvegegmlgkrgilaeifkaLaeeeinvdlisqtese...tsislvvdeedvdeakkaLkeesgaaele 335 + g+ +k+g++ +i + +++++i +d+i+q ++ t+ ++++++ d++ a ++Lk++ ++++ + lcl|NCBI__GCF_000483485.1:WP_028486187.1 269 MIL--GVPDKPGVAYQILGPISDANIEIDMIIQNQGIdgtTDFTFTIARGDLPVALDLLKSTANKLGAR 335 ***..9****************************986778***************************** PP TIGR00656 336 sleveedlavvsivgaglveapGvaseifkaleekninilmisssetkisvlvdekdaekavrklhekl 404 ++ + + ++s+vg+g+++++G+as++fk l++++ini mi++ e+kisv++de+++e av++lhe++ lcl|NCBI__GCF_000483485.1:WP_028486187.1 336 EVLSDDTIVKISMVGVGMKSHSGIASQMFKTLADNDINIQMIGTTEIKISVVIDEQYLELAVQSLHEAF 404 ********************************************************************* PP TIGR00656 405 ee 406 e+ lcl|NCBI__GCF_000483485.1:WP_028486187.1 405 EL 406 97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (407 nodes) Target sequences: 1 (408 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 6.92 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory