Align acetohydroxyacid synthase subunit B (EC 2.2.1.6) (characterized)
to candidate WP_028486983.1 B076_RS0108815 acetolactate synthase 3 large subunit
Query= metacyc::MONOMER-18810 (585 letters) >NCBI__GCF_000483485.1:WP_028486983.1 Length = 567 Score = 763 bits (1970), Expect = 0.0 Identities = 377/566 (66%), Positives = 448/566 (79%), Gaps = 4/566 (0%) Query: 18 EMIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQTK-FEHILVRHEQAAVHAADGY 76 EM GA+ILV+ L +EGVE++WGYPGGAVL IYD L K HILVRHEQAAVHAADGY Sbjct: 2 EMTGAQILVNYLEDEGVEHIWGYPGGAVLPIYDALDTDAKKLSHILVRHEQAAVHAADGY 61 Query: 77 ARATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGI 136 AR+TGK GV LVTSGPG TNAVTGIATAY+DSIPMV ITG VPT IG DAFQE DTVGI Sbjct: 62 ARSTGKPGVVLVTSGPGATNAVTGIATAYMDSIPMVCITGQVPTALIGLDAFQEIDTVGI 121 Query: 137 TRPIVKHNFLVKDVRDLAATIKKAFFIAATGRPGPVVVDIPKDVSRNACKYEYPKSIDMR 196 TRPIVKHNFLVK V +LA+T+KKAF++A TGRPGPVV+DIPKD+ Y YP+ ++MR Sbjct: 122 TRPIVKHNFLVKSVDELASTLKKAFYLATTGRPGPVVIDIPKDIQNAKSTYVYPQEVEMR 181 Query: 197 SYNPVNKGHSGQIRKAVALLQGAERPYIYTGGGVVLANASDELRQLAALTGHPVTNTLMG 256 SY PV KGHSGQI+KAV ++ A+RP +YTGGGV+L +AS+EL +L G P+T TLMG Sbjct: 182 SYLPVTKGHSGQIKKAVEMMLSAKRPILYTGGGVILGDASEELIKLTHKLGFPITQTLMG 241 Query: 257 LGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPAHFTSQARKIIH 316 LGAFP + KQ VGMLGMHGTYEAN++M + DV+IAIGARFDDRV GN F A KI+H Sbjct: 242 LGAFPASDKQSVGMLGMHGTYEANLSMHHSDVIIAIGARFDDRVTGNLEKFCPDA-KIVH 300 Query: 317 IDIDPSSISKRVKVDIPIVGNVKDVLQELIAQIKASDIKPKREALAKWWEQIEQWRSVDC 376 +DIDP+SISK V VDIPIVG VK VL E+ A + + K EALA+WW+QIEQWR+ C Sbjct: 301 VDIDPASISKNVIVDIPIVGPVKQVLTEMNAILDKTKQKVDEEALAEWWQQIEQWRATKC 360 Query: 377 LKYDRSSEIIKPQYVVEKIWELTKGDAFICSDVGQHQMWAAQFYKFDEPRRWINSGGLGT 436 L+YD + IKPQ +E +WE+T GDA++ SDVGQHQM+AAQ+Y FD+PRRWINSGGLGT Sbjct: 361 LRYDTAGSKIKPQAAMEAVWEVTNGDAYVASDVGQHQMFAAQYYPFDKPRRWINSGGLGT 420 Query: 437 MGVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQYDTPVKICSLNNGYLGMVR 496 MG GLP AMG + AFP+ V+ +TGEGSIQM IQELSTCLQY PVK+ LNNG+LGMVR Sbjct: 421 MGFGLPAAMGAQMAFPDATVICVTGEGSIQMNIQELSTCLQYGLPVKVVCLNNGFLGMVR 480 Query: 497 QWQEIEYDNRYSHSYMDALPDFVKLAEAYGHVGMRVEKTSDVEPALREAF--RLKDRTVF 554 QWQE Y+ RYS SYM++LPDFVKLAE+YGHVGMR+E ++ L EAF + KDR VF Sbjct: 481 QWQEFFYERRYSMSYMESLPDFVKLAESYGHVGMRIEDPKTMKAQLEEAFSDKNKDRFVF 540 Query: 555 LDFQTDPTENVWPMVQAGKGISEMLL 580 +D TD ENV+PM+ AG G++EM+L Sbjct: 541 IDIITDQQENVYPMIPAGAGLNEMIL 566 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1018 Number of extensions: 39 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 585 Length of database: 567 Length adjustment: 36 Effective length of query: 549 Effective length of database: 531 Effective search space: 291519 Effective search space used: 291519 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
Align candidate WP_028486983.1 B076_RS0108815 (acetolactate synthase 3 large subunit)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.17589.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.8e-270 883.5 2.6 3.1e-270 883.3 2.6 1.0 1 lcl|NCBI__GCF_000483485.1:WP_028486983.1 B076_RS0108815 acetolactate synt Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000483485.1:WP_028486983.1 B076_RS0108815 acetolactate synthase 3 large subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 883.3 2.6 3.1e-270 3.1e-270 1 555 [. 3 566 .. 3 567 .] 0.97 Alignments for each domain: == domain 1 score: 883.3 bits; conditional E-value: 3.1e-270 TIGR00118 1 lkgaeilveslkkegvetvfGyPGGavlpiydaly..dselehilvrheqaaahaadGyarasGkvGvv 67 ++ga+ilv+ l++egve+++GyPGGavlpiydal +++l+hilvrheqaa+haadGyar++Gk+Gvv lcl|NCBI__GCF_000483485.1:WP_028486983.1 3 MTGAQILVNYLEDEGVEHIWGYPGGAVLPIYDALDtdAKKLSHILVRHEQAAVHAADGYARSTGKPGVV 71 79********************************987889***************************** PP TIGR00118 68 latsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpe 136 l+tsGPGatn+vtgiatay+ds+P+v +tGqv+t+liG dafqeid +Git+p++kh+flvk++++l++ lcl|NCBI__GCF_000483485.1:WP_028486983.1 72 LVTSGPGATNAVTGIATAYMDSIPMVCITGQVPTALIGLDAFQEIDTVGITRPIVKHNFLVKSVDELAS 140 ********************************************************************* PP TIGR00118 137 ilkeafeiastGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvl 205 +lk+af++a+tGrPGPv++d+Pkd+++a+ ++ ++++ve+++y p +kgh qikka+e++ +ak+P+l lcl|NCBI__GCF_000483485.1:WP_028486983.1 141 TLKKAFYLATTGRPGPVVIDIPKDIQNAKSTYVYPQEVEMRSYLPVTKGHSGQIKKAVEMMLSAKRPIL 209 ********************************************************************* PP TIGR00118 206 lvGgGviiaeaseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliavG 274 + GgGvi ++aseel +l+++l p+t+tl+GlGafp+ +++++gmlGmhGt+eanl+++++d++ia+G lcl|NCBI__GCF_000483485.1:WP_028486983.1 210 YTGGGVILGDASEELIKLTHKLGFPITQTLMGLGAFPASDKQSVGMLGMHGTYEANLSMHHSDVIIAIG 278 ********************************************************************* PP TIGR00118 275 arfddrvtgnlakfapeakiihididPaeigknvkvdipivGdakkvleellkklkee.....ekkeke 338 arfddrvtgnl+kf+p+aki+h+didPa+i+knv vdipivG +k+vl+e+ + l ++ e+ e lcl|NCBI__GCF_000483485.1:WP_028486983.1 279 ARFDDRVTGNLEKFCPDAKIVHVDIDPASISKNVIVDIPIVGPVKQVLTEMNAILDKTkqkvdEEALAE 347 **************************************************9877666544353333446 PP TIGR00118 339 WlekieewkkeyilkldeeeesikPqkvikelskllkdeaivttdvGqhqmwaaqfyktkkprkfitsg 407 W+++ie+w+++++l++d+ ++ikPq+ +++++++++++a+v++dvGqhqm+aaq+y+++kpr++i+sg lcl|NCBI__GCF_000483485.1:WP_028486983.1 348 WWQQIEQWRATKCLRYDTAGSKIKPQAAMEAVWEVTNGDAYVASDVGQHQMFAAQYYPFDKPRRWINSG 416 ********************************************************************* PP TIGR00118 408 GlGtmGfGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWqel 476 GlGtmGfGlPaa+Ga++a p++tv++vtG+gs+qmn+qelst+ +y++pvk+v+lnn +lGmv+qWqe+ lcl|NCBI__GCF_000483485.1:WP_028486983.1 417 GLGTMGFGLPAAMGAQMAFPDATVICVTGEGSIQMNIQELSTCLQYGLPVKVVCLNNGFLGMVRQWQEF 485 ********************************************************************* PP TIGR00118 477 fyeerysetklaselpdfvklaeayGvkgiriekpeeleeklkealesk...epvlldvevdkeeevlP 542 fye+rys ++++s lpdfvklae+yG++g+rie+p+ ++++l+ea++ k + v++d+ d++e+v+P lcl|NCBI__GCF_000483485.1:WP_028486983.1 486 FYERRYSMSYMES-LPDFVKLAESYGHVGMRIEDPKTMKAQLEEAFSDKnkdRFVFIDIITDQQENVYP 553 ************5.*******************************998756689*************** PP TIGR00118 543 mvapGagldelve 555 m++ Gagl+e++ lcl|NCBI__GCF_000483485.1:WP_028486983.1 554 MIPAGAGLNEMIL 566 ***********95 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (567 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 11.04 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory