Alignments for a candidate for aspS2 in Haloglycomyces albus DSM 45210

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Haloglycomyces albus DSM 45210

Align aspartate-tRNAAsn ligase (EC 6.1.1.23) (characterized)
to candidate WP_025274508.1 HALAL_RS0113505 aspartate--tRNA ligase

Query= BRENDA::Q9KDG1
         (595 letters)



>NCBI__GCF_000527155.1:WP_025274508.1
          Length = 591

 Score =  564 bits (1453), Expect = e-165
 Identities = 296/578 (51%), Positives = 392/578 (67%), Gaps = 10/578 (1%)

Query: 4   RTHHCGQLSEEQVNERVQLKGWVQRRRDLGQVIFVDLRDRSGVVQLVFNSDISQEALETA 63
           RTH  G L  E  ++ V L GWV RRRD G V FVDLRD SGVVQ+VF      EA E A
Sbjct: 3   RTHRAGTLRAEHADQEVTLAGWVARRRDHGGVEFVDLRDSSGVVQIVFRDS---EAAEAA 59

Query: 64  EKVRNEYVLDVEGVVLKRDPSTVNDKIATGTIEVHVERLTILNKAKSLPFQIEANTDASE 123
           +K+RNEY + + G V  R     N +I TG+IEV V  LT+L+ A+ LPFQI+ + D SE
Sbjct: 60  QKLRNEYCIQITGTVAIRPDGNENPEIPTGSIEVSVADLTVLSVAEPLPFQIDDHIDVSE 119

Query: 124 DIRLKYRYLDLRRPDMQETMKLRHQTTKLIRDFLDGQEFFEIETPMLTKSTPEGARDYLV 183
           ++RL YRYLDLRR      ++ R Q TK+ RD L  ++F E+ETP LT+STPEGARD+LV
Sbjct: 120 EVRLHYRYLDLRRSGPSRALRTRSQATKIARDILAEEDFLEVETPTLTRSTPEGARDFLV 179

Query: 184 PSRVHHGEFYALPQSPQIFKQLLMVSGFERYYQIVRCFRDEDLRADRQPEFTQIDIETSF 243
           P R+  G +YALPQSPQ+FKQLLMV GFERYYQI RC+RDED RADRQPEFTQ+DIE +F
Sbjct: 180 PVRLQPGSWYALPQSPQLFKQLLMVGGFERYYQIARCYRDEDFRADRQPEFTQLDIEAAF 239

Query: 244 MDKEDLLTMTENMMAKIMKEVKGLDVALPFPRMTYDDAMNRYGSDKPDTRFEMELIELSD 303
           + +ED++++ E ++A + KE+   D+  P P +T+ +AMNR+GSDKPD RF  EL EL+D
Sbjct: 240 VSQEDIISIGEKIVAGLWKELADYDIPTPIPHLTWHEAMNRFGSDKPDLRFGQELTELTD 299

Query: 304 IVKDSDFKVFSSAIKSGGIVKGLNLKGGAGSLSRKEIDGLAEFVKPYGAKGLAW--LKVE 361
            ++ ++F+VF+ AI  GG V  + + GGA   +RKE+DG  ++ K  GAKGLA+  L  E
Sbjct: 300 YLRGTEFRVFAGAIDMGGYVGAVVMPGGA-EQTRKELDGWQDWAKARGAKGLAYVTLNAE 358

Query: 362 EGELKGPIAKFFAGETGAELQQAMGAEDGDLLFFAA--DKKEVVFDSLGALRLKLGKDFN 419
            GE KGP+AK  + E    L +A GA+ GD +FFAA  D++E   + LGA RL++G+  +
Sbjct: 359 TGEPKGPVAKNLSEEHLNGLAEATGAKPGDAVFFAASPDRREAQ-ELLGAARLEIGRRRD 417

Query: 420 LIDESKFNFLWVVDFPLVEYDEEAKRFVALHHPFTSPKQEDLTKLETDPASVRADAYDLV 479
           LID   + F W+VD P+ E  ++   + A+HHPFTSP  + + K E DP S     YD+V
Sbjct: 418 LIDLEAWAFCWIVDAPMFEPTDDG-GWSAVHHPFTSPNPDWVEKFEQDPGSALTYGYDIV 476

Query: 480 LNGYELGGGSQRIYQRPVQEKMFAALGFTEEAAQKEFGFLLEAFEYGTPPHGGIALGLDR 539
            NG E+GGGS RI+   +Q ++F  LG T E A ++FGFLLEAF++G PPHGGIA G DR
Sbjct: 477 CNGNEIGGGSIRIHDGRLQSRVFDVLGITPEEAHEKFGFLLEAFKFGPPPHGGIAFGWDR 536

Query: 540 LVMLLAGRLNLRDTIAFPKTASASCLLTEAPGEVSLEQ 577
           +  LL G  ++R+ IAFPKT      LT AP  ++ EQ
Sbjct: 537 VCSLLTGSDSIREVIAFPKTGGGHDPLTGAPTPITAEQ 574


Lambda     K      H
   0.319    0.138    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 934
Number of extensions: 46
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 595
Length of database: 591
Length adjustment: 37
Effective length of query: 558
Effective length of database: 554
Effective search space:   309132
Effective search space used:   309132
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis