Alignments for a candidate for ilvI in Thiothrix lacustris DSM 21227

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Thiothrix lacustris DSM 21227

Align Probable acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized)
to candidate WP_028488024.1 Q394_RS0103325 acetolactate synthase

Query= curated2:O08353
         (599 letters)



>NCBI__GCF_000621325.1:WP_028488024.1
          Length = 557

 Score =  259 bits (661), Expect = 3e-73
 Identities = 172/554 (31%), Positives = 295/554 (53%), Gaps = 46/554 (8%)

Query: 4   AEAMIKALEAEKVEILFGYPGGALLPFYDALHHSDLIHLLTRHEQAAAHAADGYARASGK 63
           +E ++K L    V+ LFG PG  +LP YD L+ S +  +L +HEQ AA  A GYAR SG+
Sbjct: 6   SELLVKFLHRLGVDTLFGMPGAHILPVYDDLYGSGIQTVLVKHEQGAAFMAGGYARVSGR 65

Query: 64  VGVCIGTSGPGATNLVTGVATAHSDSSPMVALTGQVPTKLIGNDAFQE-------IDALG 116
           +G CI T+GPGA+NL+TG+A A++D  P++ +TG+ PT + G    QE       ID   
Sbjct: 66  IGACITTAGPGASNLITGIANAYADKLPVLVITGEAPTYIFGRGGLQESSGEGGSIDQTA 125

Query: 117 LFMPIVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKDVQELELDI-------D 169
           LF  + +++  I++T  +  +   A     +  PGPV + +P +VQ+  +D        D
Sbjct: 126 LFSGVTRYHKLIERTDYLVNVLNQAARQLVSDVPGPVVLSIPVNVQKELVDASILDKLPD 185

Query: 170 KHPIPSKVKLIGYNPTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSG-ANEELLKLVE 228
            HP+P K+K+          P+ +++   +I SAKRP++LAG G + S  A++ L +  +
Sbjct: 186 LHPLP-KLKIA---------PQTLEQCGDMIRSAKRPVVLAGYGCIQSATAHDALCEFSQ 235

Query: 229 LLNIPVCTTLMGKGCISENHPLALGMVGMHGTKPA-NYCLSESDVLISIGCRFSDRITGD 287
            LNIPV ++L GKG I E   L+LG +G+  +  A +Y + E+D++I +G  F++R +  
Sbjct: 236 RLNIPVASSLKGKGAIDERSALSLGSLGVTSSGHAMHYFMHEADLIILLGAGFNERTSYV 295

Query: 288 IKSFATNAK-IIHIDIDPAEIGKNVNVDVPIVGDAKLILKEVIKQLDYIINKDSKENNDK 346
             +   N K II +D +  ++ K    D+ I  D    L+         IN    +   +
Sbjct: 296 WNAALLNGKKIIQVDRNVTQLEKVFKADLAIQSDLGDFLQS--------INGWCAQQVIE 347

Query: 347 ENISQWIENVNSLKKSSIPVMDYDDIPIKPQK--IVKELMAVIDDLNINKNTIITTDVGQ 404
             I     ++ + K+     M+ D   I  ++  +VK   A+++    N   ++  D   
Sbjct: 348 PKICP---DLAAFKRGIDQQMEADGTVIFGEQFDLVKTFFALLEQ-QFNDGIVLVDD--- 400

Query: 405 NQMWMAHYFKTQTPRSFLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQE 464
           N ++  ++++      F+ + G+ ++G   P+AIG++    D     + GDGGF M C E
Sbjct: 401 NIIFAQNFYRVTPQDHFVPNTGVSSLGHAIPAAIGSRFTV-DKPTFAVIGDGGFQMCCME 459

Query: 465 LGTIAEYNIPVVICIFDNRTLGMVYQWQNLFYGKRQCSVNFGGAPDFIKLAESYGIKARR 524
           + T   Y+IP+ I +F+N+TLG++ + Q+  Y +R    +F   PD+  LA+S+GI   +
Sbjct: 460 IMTAVNYHIPLNIVLFNNQTLGLIRKNQHQQYEQRFLDCDFKN-PDYALLAQSFGINHFK 518

Query: 525 IESPNEINEALKEA 538
           IE+  +  +   +A
Sbjct: 519 IETDADCEQVFAQA 532


Lambda     K      H
   0.319    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 747
Number of extensions: 53
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 599
Length of database: 557
Length adjustment: 36
Effective length of query: 563
Effective length of database: 521
Effective search space:   293323
Effective search space used:   293323
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis