Align Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.- (characterized)
to candidate WP_029912908.1 P166_RS0111585 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB
Query= SwissProt::Q9RF06 (475 letters) >NCBI__GCF_000711315.1:WP_029912908.1 Length = 475 Score = 462 bits (1188), Expect = e-134 Identities = 230/475 (48%), Positives = 330/475 (69%), Gaps = 4/475 (0%) Query: 1 MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAM 60 M +E VIGLE+H +L T++K+FS S +GAEPN+ N++DL PG LPV+NK + A+ Sbjct: 1 MTWEVVIGLEIHAQLNTNTKIFSGSSIAYGAEPNTQANLLDLGMPGQLPVLNKAVLPKAI 60 Query: 61 RAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPI-GENGYIDIEVDGETKRIGIT 119 R +A+ I S FDRKNYFYPD PK YQ SQF PI + G ++IEVDGETK+IGIT Sbjct: 61 RFGLAVGATIGRRSVFDRKNYFYPDLPKGYQTSQFSYPIVDKGGVLEIEVDGETKKIGIT 120 Query: 120 RLHMEEDAGKSTHKG--EYSLVDLNRQGTPLIEIVSEPDIRSPKEAYAYLEKLRSIIQYT 177 R H+EEDAGKS H + +DLNR GTPL+EIVSEPD+RS +EA AY K+ ++QY Sbjct: 121 RAHLEEDAGKSVHDAFPGQTGIDLNRAGTPLLEIVSEPDMRSAQEAVAYARKMHELVQYL 180 Query: 178 GVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFNYVRKGLEYEEKRQEEELLS 237 G+ D M+EGS R D+N+S+ G FGT+AELKN+NSF ++ + +EYE RQ E L S Sbjct: 181 GICDGNMQEGSFRVDSNVSIHKPGTP-FGTRAELKNINSFKFIEQAIEYEIARQIEILES 239 Query: 238 GGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKERVRQTIPELPD 297 GG++ QETR +D +T MR KE ++DYRYFP PD++P+ + D E +R +PELP Sbjct: 240 GGKVVQETRLYDSEKNETRSMRAKEEANDYRYFPCPDLLPVVVTDEDIEAIRAEMPELPG 299 Query: 298 ERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHGADVKLTSNWLMGGVNEYLNKNQV 357 ++A+YV+E GL YDA LT ++ M+++FE+ + H D K+++NW+MG +++ LN+ + Sbjct: 300 AKRARYVSEFGLSDYDASFLTGSRAMAEYFEAVVSHSKDAKMSANWVMGELSKSLNQQGI 359 Query: 358 ELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNAKQIMEDNGLVQISDEAT 417 ++ ++ ++ E LAG++ I+D T+S KIAK+VF + + G+A +I+ GL QI+D + Sbjct: 360 DINESPVSAEALAGLLARIQDNTISGKIAKQVFDAMWNQEGSADEIITAKGLKQITDSSA 419 Query: 418 LLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLKQEL 472 + V+E L N VE Y+NG+ K G+ VGQ+MK S+GQANP VN++LK++L Sbjct: 420 IEALVDEVLAANTAQVEAYRNGQDKMFGYFVGQVMKVSQGQANPGQVNEILKRKL 474 Lambda K H 0.313 0.133 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 636 Number of extensions: 27 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 475 Length of database: 475 Length adjustment: 33 Effective length of query: 442 Effective length of database: 442 Effective search space: 195364 Effective search space used: 195364 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 51 (24.3 bits)
Align candidate WP_029912908.1 P166_RS0111585 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB)
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00133.hmm # target sequence database: /tmp/gapView.8884.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00133 [M=481] Accession: TIGR00133 Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.5e-175 569.1 0.0 3.9e-175 568.9 0.0 1.0 1 lcl|NCBI__GCF_000711315.1:WP_029912908.1 P166_RS0111585 Asp-tRNA(Asn)/Glu Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000711315.1:WP_029912908.1 P166_RS0111585 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 568.9 0.0 3.9e-175 3.9e-175 3 480 .. 2 474 .. 1 475 [] 0.98 Alignments for each domain: == domain 1 score: 568.9 bits; conditional E-value: 3.9e-175 TIGR00133 3 eyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalnsk 71 +e+viGlE+H qlnt++K+F+ +s + +pNt+ + ++lg+PG lPvlNk ++ kA+ +la+++ lcl|NCBI__GCF_000711315.1:WP_029912908.1 2 TWEVVIGLEIHAQLNTNTKIFSGSSIAYGA-EPNTQANLLDLGMPGQLPVLNKAVLPKAIRFGLAVGAT 69 689*************************99.************************************** PP TIGR00133 72 ivsevsvFdRKhYfYpDlPkgyqitqqdlPia.edGkleieleekekeigierlhlEeDtgksqykesd 139 i svFdRK+YfYpDlPkgyq +q+ +Pi+ ++G+leie++++ k+igi+r hlEeD+gks++ lcl|NCBI__GCF_000711315.1:WP_029912908.1 70 IG-RRSVFDRKNYFYPDLPKGYQTSQFSYPIVdKGGVLEIEVDGETKKIGITRAHLEEDAGKSVHDAF- 136 65.69**************************9346789***************************955. PP TIGR00133 140 kdkqslvDfNRsgvPLlEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDvNvsirlkG 208 + q+ +D+NR+g+PLlEiV++Pd++sa+ea+a+ +k++++++yl+i dg+++eGs+RvD Nvsi+ k lcl|NCBI__GCF_000711315.1:WP_029912908.1 137 -PGQTGIDLNRAGTPLLEIVSEPDMRSAQEAVAYARKMHELVQYLGICDGNMQEGSFRVDSNVSIH-KP 203 .79***************************************************************.66 PP TIGR00133 209 qekegtrvEiKNlnslksiekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKeeseDYRYf 277 + +gtr+E+KN+ns+k ie+aieyEi+Rq+++l++g +v+qetr +d +k+ t s+R Kee++DYRYf lcl|NCBI__GCF_000711315.1:WP_029912908.1 204 GTPFGTRAELKNINSFKFIEQAIEYEIARQIEILESGGKVVQETRLYDSEKNETRSMRAKEEANDYRYF 272 789****************************************************************** PP TIGR00133 278 pePdlppieideevvkekveeklpelPeakrirlkkeyglseedakvlvsdlelldafeevvklikepk 346 p Pdl p+++++e ++ +++++pelP akr+r+++e+gls++da l+ + ++++fe vv+ +k++k lcl|NCBI__GCF_000711315.1:WP_029912908.1 273 PCPDLLPVVVTDEDIEA-IRAEMPELPGAKRARYVSEFGLSDYDASFLTGSRAMAEYFEAVVSHSKDAK 340 ***************99.*************************************************** PP TIGR00133 347 lavnWileellgeLnkkkislaeallkpeelaeliklikegkisqksakelleellenkkdpkkliekl 415 ++nW++ el ++Ln++ i+++e+ +++e+la l++ i++++is+k+ak++++ + +++++++++i+++ lcl|NCBI__GCF_000711315.1:WP_029912908.1 341 MSANWVMGELSKSLNQQGIDINESPVSAEALAGLLARIQDNTISGKIAKQVFDAMWNQEGSADEIITAK 409 ********************************************************************* PP TIGR00133 416 gliqisdekelvkiveevikenpkevekyksgkekalkflvGqvmkktkgradpkevekllkell 480 gl qi+d +++ ++v+ev++ n+ +ve y++g++k+++++vGqvmk ++g+a+p +v+++lk +l lcl|NCBI__GCF_000711315.1:WP_029912908.1 410 GLKQITDSSAIEALVDEVLAANTAQVEAYRNGQDKMFGYFVGQVMKVSQGQANPGQVNEILKRKL 474 **************************************************************998 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (481 nodes) Target sequences: 1 (475 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 12.05 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory