Alignments for a candidate for serB in Beijerinckia mobilis UQM 1969

GapMind for Amino acid biosynthesis

Alignments for a candidate for serB in Beijerinckia mobilis UQM 1969

Align Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 (characterized)
to candidate WP_034991023.1 DL88_RS02095 phosphoserine phosphatase SerB

Query= SwissProt::Q12A06
         (236 letters)



>NCBI__GCF_000745425.1:WP_034991023.1
          Length = 325

 Score =  155 bits (391), Expect = 1e-42
 Identities = 97/222 (43%), Positives = 133/222 (59%), Gaps = 6/222 (2%)

Query: 12  VNVATPDLKLSDFKLIAFDMDSTLINIECVDEIADAAGRKAEVAAITEAAMRGEISDYKE 71
           V+V    L+    KL   DMDST+I  EC+DE+A   G+K  VA ITE AMRGEI+ ++ 
Sbjct: 70  VDVIVQPLENRRKKLFLADMDSTMIRQECIDELAGEVGKKKHVAEITERAMRGEIA-FEP 128

Query: 72  SLRQRVALLKGVSVASMDEVYRTRLRLNPGAARLVQACKDAGLKVLLVSGGFTFFTDRIR 131
           +LR+RVALLKG+   ++ +V   R+ ++PGA  LVQ  +  G+  +LVSGGFT FT  I 
Sbjct: 129 ALRERVALLKGLHPDTIRKVIGKRITMSPGARILVQTLRQHGVHTVLVSGGFTAFTSVIG 188

Query: 132 DELGIDYTRSNVLET-TDGLLTGRMVDQPWGDICDGEEKRKMLLETCGQLGISPRQAIAM 190
           +++G     +NVL    DG L G +V +P   I   + K   L+    +L ++    IA+
Sbjct: 189 EQIGFHENHANVLLLGQDGRLAG-LVQEP---ILGKDAKLATLVAKRDELKLAESDTIAV 244

Query: 191 GDGANDLPMMGEAGLSVAYHAKPRVREQAMVAINEGGLDRLL 232
           GDGANDL M+  AGL +AYHAKP V   A   I+ G L  LL
Sbjct: 245 GDGANDLDMIRAAGLGLAYHAKPAVAAAAAACIDHGDLTALL 286


Lambda     K      H
   0.319    0.136    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 200
Number of extensions: 13
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 236
Length of database: 325
Length adjustment: 25
Effective length of query: 211
Effective length of database: 300
Effective search space:    63300
Effective search space used:    63300
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

Align candidate WP_034991023.1 DL88_RS02095 (phosphoserine phosphatase SerB)
to HMM TIGR00338 (serB: phosphoserine phosphatase SerB (EC 3.1.3.3))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00338.hmm
# target sequence database:        /tmp/gapView.20008.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00338  [M=219]
Accession:   TIGR00338
Description: serB: phosphoserine phosphatase SerB
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    5.7e-66  208.3   0.2    7.6e-66  207.8   0.2    1.1  1  lcl|NCBI__GCF_000745425.1:WP_034991023.1  DL88_RS02095 phosphoserine phosp


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000745425.1:WP_034991023.1  DL88_RS02095 phosphoserine phosphatase SerB
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  207.8   0.2   7.6e-66   7.6e-66      11     217 ..      79     287 ..      71     288 .. 0.96

  Alignments for each domain:
  == domain 1  score: 207.8 bits;  conditional E-value: 7.6e-66
                                 TIGR00338  11 lkkkklvvfDlDstlieeEvIdeiaklaGveeeVseiTerAmrgeldFkeslreRvkllkglpve.llk 78 
                                               ++ kkl++ D+Dst+i++E+Ide+a ++G +++V+eiTerAmrge+ F+ +lreRv+llkgl+ + ++k
  lcl|NCBI__GCF_000745425.1:WP_034991023.1  79 NRRKKLFLADMDSTMIRQECIDELAGEVGKKKHVAEITERAMRGEIAFEPALRERVALLKGLHPDtIRK 147
                                               5789***********************************************************995666 PP

                                 TIGR00338  79 kveeklelteGveelvkkLkekgykvaviSGgFdlvaeklkekLgldavfaNrLeve.dgkltGkvege 146
                                                + +++++ +G++ lv++L+++g++++++SGgF+ ++  + e++g     aN L +  dg+l+G v+ +
  lcl|NCBI__GCF_000745425.1:WP_034991023.1 148 VIGKRITMSPGARILVQTLRQHGVHTVLVSGGFTAFTSVIGEQIGFHENHANVLLLGqDGRLAGLVQEP 216
                                               77899************************************************99988*********** PP

                                 TIGR00338 147 ivdesakaktllkllekegislektvavGDGanDlsmikaAglgiafnakpvlkekadiviekkdltdi 215
                                               i+ ++ak  tl+ +  + ++   +t+avGDGanDl mi+aAglg+a++akp++  +a ++i++ dlt++
  lcl|NCBI__GCF_000745425.1:WP_034991023.1 217 ILGKDAKLATLVAKRDELKLAESDTIAVGDGANDLDMIRAAGLGLAYHAKPAVAAAAAACIDHGDLTAL 285
                                               *******************************************************************99 PP

                                 TIGR00338 216 le 217
                                               l+
  lcl|NCBI__GCF_000745425.1:WP_034991023.1 286 LY 287
                                               75 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (219 nodes)
Target sequences:                          1  (325 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 11.98
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis