Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (characterized)
to candidate WP_038213205.1 Q392_RS23315 dihydroxy-acid dehydratase
Query= SwissProt::P9WKJ5 (575 letters) >NCBI__GCF_000745855.1:WP_038213205.1 Length = 582 Score = 468 bits (1205), Expect = e-136 Identities = 253/567 (44%), Positives = 347/567 (61%), Gaps = 17/567 (2%) Query: 17 IKPRSRDVTDGLEKAAARGMLRAVGMDDEDFAKPQIGVASSWNEITPCNLSLDRLANAVK 76 + PRS + G +A R LRA+G DDED A+P +GV + E++PCNL+L A K Sbjct: 10 LPPRSATIHTGTIRATTRAFLRALGQDDEDIARPHVGVFHTGGEMSPCNLNLREQAQHAK 69 Query: 77 EGVFSAGGYPLEFGTISVSDGISMGHEGMHFSLVSREVIADSVEVVMQAERLDGSVLLAG 136 G+++AGG P E +SVSDG++M H GM FSL+SRE+IADSVE ++ + DG + Sbjct: 70 TGIYAAGGTPHECPVVSVSDGLTMAHSGMRFSLISRELIADSVEASVRGHQWDGVFAIGA 129 Query: 137 CDKSLPGMLMAAARLDLAAVFLYAGSILPGRAKLSDGSERDVTIIDAFEAVGACSRGLMS 196 CDK+LPG++M R ++ VF++ G+ LPGR RD+ ++D +E +G G + Sbjct: 130 CDKNLPGLMMGIVRCNVPGVFVHGGAALPGRI---GPHGRDLNVVDTYETIGKLLAGEAT 186 Query: 197 RADVDAIERAICPGEGACGGMYTANTMASAAEALGMSLPGSAAPPATDRRRDGFARRSGQ 256 +++ + RA P G+C G +TANTM +EALG++ GS+ PA R RR+ + Sbjct: 187 AEELETMSRACLPTAGSCAGQFTANTMGMVSEALGLAPIGSSMVPAVYSERAPLMRRAAK 246 Query: 257 AVVELLRRGITA-----RDILTKEAFENAIAVVMAFGGSTNAVLHLLAIAHEANVALSLQ 311 ++ + + RDI+T++A ENA AVV A GGSTNA LH+ AIAHEA + L Sbjct: 247 QLMRAVSASLEGGGPLPRDIVTRQALENACAVVCATGGSTNAALHIPAIAHEAGIRFHLD 306 Query: 312 DFSRIGSGVPHLADVKPFGRHVMSDVDHIGGVPVVMKALLDAGLLHGDCLTVTGHTMAEN 371 D + + + P +A++ P G+++ DV IGG VV++ALLD G LHGD LT TG T+AE Sbjct: 307 DVAEVFARTPLIANLSPGGKYLARDVYSIGGAGVVLRALLDQGHLHGDALTWTGRTLAEE 366 Query: 372 LAAITPPDPDGKVLRALANPIHPSGGITILHGSLAPEGAVVKTAGFDSDVFEGTARVFDG 431 LA +PDG+V+R PI P GG+T+L G+L P+GA++KTAG + V EG ARVF+ Sbjct: 367 LAGCA--EPDGEVVRRAGEPISPDGGLTVLKGNLCPDGALLKTAGLKTLVHEGPARVFEN 424 Query: 432 ERAALDALEDGTITVGDAVVIRYEGPKGGPGMREMLAITGAIKGAGLGKDVLLLTDGRFS 491 E A A+++ GD +VIR EGPKG PGMREML IT + G G+G V LLTDGRFS Sbjct: 425 EEEAQAAVQNRRYQAGDVIVIRNEGPKGSPGMREMLGITALLYGQGMGDKVALLTDGRFS 484 Query: 492 GGTTGLCVGHIAPEAVDGGPIALLRNGDRIRLDV--AGRVLDVLADPAEFASR---QQDF 546 G T GLC+G+ PEA GGPIA LR+GDRI +D R + V E R + Sbjct: 485 GATRGLCIGYAGPEAAAGGPIAALRDGDRIAIDARPGARSITVALPAEEIVMRLNQKAGV 544 Query: 547 SPPPPRYTTGVLSKYVKLVSSAAVGAV 573 + PR G+L KY V A GAV Sbjct: 545 NEGAPR--GGLLEKYALTVRPAHQGAV 569 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1050 Number of extensions: 55 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 575 Length of database: 582 Length adjustment: 36 Effective length of query: 539 Effective length of database: 546 Effective search space: 294294 Effective search space used: 294294 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory