Align Acetolactate synthase isozyme 1 small subunit; EC 2.2.1.6; Acetohydroxy-acid synthase I small subunit; AHAS-I; ALS-I (uncharacterized)
to candidate WP_035235593.1 Q366_RS01300 acetolactate synthase small subunit
Query= curated2:P0ADG0 (96 letters) >NCBI__GCF_000745975.1:WP_035235593.1 Length = 93 Score = 85.5 bits (210), Expect = 1e-22 Identities = 41/85 (48%), Positives = 58/85 (68%), Gaps = 1/85 (1%) Query: 8 NVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPIQDSDKSHIWLLVNDDQRLEQMIS 67 + I+ELTVRNHPGVM+ + GLFARR FN+EGILC P D S + L++ D+R EQ++ Sbjct: 3 DTIVELTVRNHPGVMSQITGLFARRNFNLEGILCRPEGDGGLSRMRLMIYTDERTEQLVK 62 Query: 68 QIDKLEDVVKVQ-RNQSDPTMFNKI 91 Q+ KL DV+ V R P+ + ++ Sbjct: 63 QLSKLYDVISVSIRQDRGPSRYRQL 87 Lambda K H 0.324 0.137 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 30 Number of extensions: 1 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 96 Length of database: 93 Length adjustment: 10 Effective length of query: 86 Effective length of database: 83 Effective search space: 7138 Effective search space used: 7138 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.1 bits) S2: 39 (19.6 bits)
Align candidate WP_035235593.1 Q366_RS01300 (acetolactate synthase small subunit)
to HMM TIGR00119 (ilvN: acetolactate synthase, small subunit (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00119.hmm # target sequence database: /tmp/gapView.26771.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00119 [M=158] Accession: TIGR00119 Description: acolac_sm: acetolactate synthase, small subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.3e-20 59.2 0.0 2.6e-20 59.0 0.0 1.0 1 lcl|NCBI__GCF_000745975.1:WP_035235593.1 Q366_RS01300 acetolactate syntha Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000745975.1:WP_035235593.1 Q366_RS01300 acetolactate synthase small subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 59.0 0.0 2.6e-20 2.6e-20 3 74 .. 4 74 .. 2 82 .. 0.93 Alignments for each domain: == domain 1 score: 59.0 bits; conditional E-value: 2.6e-20 TIGR00119 3 hvlsvlvenepGvLsrvsGlfarrgfniesltvgeteekdlsrmtivvegddkvveqiekqleklvdvlkv 73 +++++v+n+pGv+s+++Glfarr+fn+e + + +lsrm +++ +d+++ eq+ kql+kl dv+ v lcl|NCBI__GCF_000745975.1:WP_035235593.1 4 TIVELTVRNHPGVMSQITGLFARRNFNLEGILCRPEGDGGLSRMRLMIYTDERT-EQLVKQLSKLYDVISV 73 58899***************************99999************99875.**************99 PP TIGR00119 74 l 74 + lcl|NCBI__GCF_000745975.1:WP_035235593.1 74 S 74 7 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (158 nodes) Target sequences: 1 (93 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 4.48 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory