Alignments for a candidate for aspS2 in Methylocapsa aurea KYG T

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Methylocapsa aurea KYG T

Align aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) (characterized)
to candidate WP_036258034.1 DL86_RS01745 aspartate--tRNA ligase

Query= reanno::Caulo:CCNA_01969
         (612 letters)



>NCBI__GCF_000746085.1:WP_036258034.1
          Length = 602

 Score =  712 bits (1837), Expect = 0.0
 Identities = 367/622 (59%), Positives = 447/622 (71%), Gaps = 34/622 (5%)

Query: 4   MHAYRTHNCGALRASDTGAAVRLSGWIHRKRDHGGLVFIDLRDHYGLTQLVLHPETPGFN 63
           MH YR+H CG LR +  G  +RLSGW HR RDHGG++FIDLRDHYG+TQ V+ P++  F 
Sbjct: 1   MHRYRSHTCGDLRETLAGETIRLSGWCHRIRDHGGVLFIDLRDHYGMTQCVVDPDSKAFA 60

Query: 64  VVERLRAESVIRVDGEVIARDASVVNPNLPTGEIEIRVSAVEVLSEAAELPLPVFGEPDY 123
           + E+LR+E V+R+DG V  R A   N  +PTG IE+ V+ +EVL  AAELP+PVFGE +Y
Sbjct: 61  LAEKLRSEWVVRIDGVVRQRPAGTENAEMPTGLIEVYVAEIEVLGPAAELPMPVFGEHNY 120

Query: 124 PEEIRLKHRYLDLRRETLHKNIVLRSRVIQSIRNRMFAQGFNEFQTPILTASSPEGARDF 183
           PE+IRL++R+LDLRRE LH+NI+LR RVI S+R RM AQGF EFQTPILTASSPEGARDF
Sbjct: 121 PEDIRLRYRFLDLRREKLHQNIMLRGRVIDSLRARMKAQGFFEFQTPILTASSPEGARDF 180

Query: 184 LVPSRLHPEKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDLRADRSL-EFYQLDVE 242
           LVPSRLHP KFYALPQAPQQFKQL+M +GFDRYFQIAPCFRDED RADRS  EFYQLDVE
Sbjct: 181 LVPSRLHPGKFYALPQAPQQFKQLVMAAGFDRYFQIAPCFRDEDARADRSPGEFYQLDVE 240

Query: 243 MSFVTQEDVFAAIEPVMHGVFEEFSNGKPVSPINGTHTFTNDFGQSFEHKGFERLTYAQS 302
           MSFVTQEDVF A+EPV+ G+FEEF  G+PV+                  + F  + YA++
Sbjct: 241 MSFVTQEDVFEAVEPVLRGIFEEFGGGQPVT------------------QKFPMIPYAEA 282

Query: 303 MAWYGSDKPDLRNPIKMANVSEHFR--DGGFGLFAKILGADAKNQVWAIPAPTGGS--RA 358
           +  YG+DKPDLRNP+ + +V++ F   D  F  F  ++   A   V AIPA   G+  R+
Sbjct: 283 LLKYGTDKPDLRNPLVIVDVTDEFSRDDVNFNAFKNVI--KAGGVVRAIPATGAGAQPRS 340

Query: 359 FCDRMNSWAQGEGQPGLGYVFW--SEDQGGWGGPIAKNLGEPTQ-ALMESLGLGAGDAAF 415
           F D++N WA+GEG  GLGYV +    D     GPIAK L    Q  +    GL AGDA F
Sbjct: 341 FFDKLNDWAKGEGAAGLGYVIFEGEGDSLAGKGPIAKFLPADVQKKIAAKAGLKAGDAVF 400

Query: 416 FVAGDPAVFAKFAGLARTRVGTELKLVDENQFKFCWIVDFPMFEWNEEEKKVDFSHNPFS 475
           F        AK AG AR R+G ELKL     F+FCWIVDFPM+EWNE+EK++DFSHNPFS
Sbjct: 401 FACDQEEKAAKLAGAARVRIGHELKLSKTGVFEFCWIVDFPMYEWNEDEKRIDFSHNPFS 460

Query: 476 MPQGGLEALETQDP------LTIRAYQYDIVCNGYELCSGAIRNHKPEIMLKAFATAGYG 529
           MP    EA    DP        ++A+QYDIVCNG EL SGAIRNH+P+IM KAFA AGYG
Sbjct: 461 MPNFESEAFLALDPADEKTIFGMKAFQYDIVCNGVELSSGAIRNHRPDIMKKAFALAGYG 520

Query: 530 PEVVEEQFGGMLNAFRYGAPPHGGLAPGIDRIVMLLADQVAIREVIAFPLNQQGQDLLMN 589
            +V+EE+FGGM  AF+YGAPPHGG+APG+DRIVMLLA +  +RE++ FP+NQ+ +DLLM 
Sbjct: 521 EDVLEEKFGGMYRAFQYGAPPHGGIAPGVDRIVMLLAGEENLREIVLFPMNQRAEDLLMG 580

Query: 590 APANVLDKQLKELHIRTAPPIK 611
           AP+ V  KQL+ELHIR   P K
Sbjct: 581 APSTVTPKQLRELHIRLNLPDK 602


Lambda     K      H
   0.321    0.139    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1131
Number of extensions: 52
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 612
Length of database: 602
Length adjustment: 37
Effective length of query: 575
Effective length of database: 565
Effective search space:   324875
Effective search space used:   324875
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis