Alignments for a candidate for asp-kinase in Nitriliruptor alkaliphilus DSM 45188

GapMind for Amino acid biosynthesis

Alignments for a candidate for asp-kinase in Nitriliruptor alkaliphilus DSM 45188

Align aspartate kinase (EC 2.7.2.4) (characterized)
to candidate WP_052664755.1 NITAL_RS03510 aspartate kinase

Query= BRENDA::P9WPX3
         (421 letters)



>NCBI__GCF_000969705.1:WP_052664755.1
          Length = 415

 Score =  479 bits (1232), Expect = e-140
 Identities = 247/410 (60%), Positives = 316/410 (77%), Gaps = 3/410 (0%)

Query: 1   MALVVQKYGGSSVADAERIRRVAERIVATKKQGNDVVVVVSAMGDTTDDLLDLAQQVCPA 60
           MA+VVQKYGG+SV D +R++RVA+R+  T + GN  VVVVSAMG TTDDL+ +AQ++  A
Sbjct: 1   MAIVVQKYGGTSVGDTDRMKRVADRVARTHRDGNQTVVVVSAMGKTTDDLVAMAQRISDA 60

Query: 61  PPPRELDMLLTAGERISNALVAMAIESLGAHARSFTGSQAGVITTGTHGNAKIIDVTPGR 120
           PP RELD+LLT+GERIS +L+AMA+   G  A+SFTGSQAG+IT   HG A+I+++TPGR
Sbjct: 61  PPQRELDILLTSGERISMSLLAMALAEHGVPAKSFTGSQAGIITDAVHGKARILEITPGR 120

Query: 121 LQTALEEGRVVLVAGFQGVSQDTKDVTTLGRGGSDTTAVAMAAALGADVCEIYTDVDGIF 180
           +Q AL+EG VV+VAGFQGVSQDTKD+T+LGRGGSDTTAVA+AAALGADVCEIYTDVDG+F
Sbjct: 121 VQEALDEGNVVIVAGFQGVSQDTKDITSLGRGGSDTTAVALAAALGADVCEIYTDVDGVF 180

Query: 181 SADPRIVRNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYSDRPGTV 240
           +ADPRIV  ARKL  V++EE LEM A GA VL  R VE+ R H + +HVRSS+S  PGT 
Sbjct: 181 TADPRIVATARKLSYVSYEEALEMTAHGAGVLQTRSVEFGRNHGVRIHVRSSFSYTPGT- 239

Query: 241 VVGSIKDVPMEDPILTGVAHDRSEAKVTIVGLPDIPGYAAKVFRAVADADVNIDMVLQNV 300
            VG  ++  +ED I++GVAHD SEAKVT++ + D PG AAK+F  +ADA +NIDM++QNV
Sbjct: 240 WVGPREESTVEDAIISGVAHDTSEAKVTVMKVSDQPGIAAKLFSTLADAKINIDMIVQNV 299

Query: 301 SKVEDGKTDITFTCSRDVGPAAVEKLDSLRNEIGFSQLLYDDHIGKVSLIGAGMRSHPGV 360
           S   DG+TDI+FT  R     A   L+     IG   +L D+++ KVSL+GAGM++HPGV
Sbjct: 300 S--ADGRTDISFTLPRGDTNQARAILEPYAEAIGAEGVLVDENVAKVSLVGAGMKTHPGV 357

Query: 361 TATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVVALHEAFGLGGD 410
            A    AL+A GVNIE+ISTS IR+SV+    +++ AV+A+HEAFGL  D
Sbjct: 358 AARMFSALSAAGVNIEMISTSTIRVSVVIAKDQVEPAVLAVHEAFGLSED 407


Lambda     K      H
   0.318    0.134    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 521
Number of extensions: 20
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 421
Length of database: 415
Length adjustment: 32
Effective length of query: 389
Effective length of database: 383
Effective search space:   148987
Effective search space used:   148987
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_052664755.1 NITAL_RS03510 (aspartate kinase)
to HMM TIGR00656 (aspartate kinase, monofunctional class (EC 2.7.2.4))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00656.hmm
# target sequence database:        /tmp/gapView.11322.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00656  [M=407]
Accession:   TIGR00656
Description: asp_kin_monofn: aspartate kinase, monofunctional class
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.9e-132  427.0  13.6   4.5e-132  426.8  13.6    1.0  1  lcl|NCBI__GCF_000969705.1:WP_052664755.1  NITAL_RS03510 aspartate kinase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000969705.1:WP_052664755.1  NITAL_RS03510 aspartate kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  426.8  13.6  4.5e-132  4.5e-132       1     406 [.       1     404 [.       1     405 [. 0.97

  Alignments for each domain:
  == domain 1  score: 426.8 bits;  conditional E-value: 4.5e-132
                                 TIGR00656   1 veliVqKFGGtsvgsserikkaakivlkelkegkkvvVVvSAmskvtdelvelaellklleaisdeisp 69 
                                               + ++VqK+GGtsvg+++r+k++a++v ++ ++g++ vVVvSAm+k+td lv++a      + isd++ +
  lcl|NCBI__GCF_000969705.1:WP_052664755.1   1 MAIVVQKYGGTSVGDTDRMKRVADRVARTHRDGNQTVVVVSAMGKTTDDLVAMA------QRISDAPPQ 63 
                                               579***************************************************......79******* PP

                                 TIGR00656  70 rerdelvsvGEllssallssalrelgvkaealdgkeagilTddefgnAkikelateerLlelLeegiiv 138
                                               re d l+  GE++s++ll++al e+gv a++++g +agi+Td+ +g+A+i e+ +  r+ e L+eg +v
  lcl|NCBI__GCF_000969705.1:WP_052664755.1  64 RELDILLTSGERISMSLLAMALAEHGVPAKSFTGSQAGIITDAVHGKARILEITP-GRVQEALDEGNVV 131
                                               *******************************************************.************* PP

                                 TIGR00656 139 vvaGFiGateeG.eiTtLGRGGSDltAallaaalkAdrveiyTDVeGvyttDPrvveeakkidkisyeE 206
                                               +vaGF+G  ++  +iT LGRGGSD+tA++laaal Ad +eiyTDV+Gv+t+DPr+v +a+k+ ++syeE
  lcl|NCBI__GCF_000969705.1:WP_052664755.1 132 IVAGFQGVSQDTkDITSLGRGGSDTTAVALAAALGADVCEIYTDVDGVFTADPRIVATARKLSYVSYEE 200
                                               *******9887638******************************************************* PP

                                 TIGR00656 207 alelAtlGakvlhpralelaveakvpilvrsskekeegTlitnkkens...slvkaialeknvarltve 272
                                               ale+  +Ga vl+ r++e+  + +v i vrss++   gT + +++e      +++++a++   a++tv+
  lcl|NCBI__GCF_000969705.1:WP_052664755.1 201 ALEMTAHGAGVLQTRSVEFGRNHGVRIHVRSSFSYTPGTWVGPREESTvedAIISGVAHDTSEAKVTVM 269
                                               ******************************************9555555559***************** PP

                                 TIGR00656 273 gegmlgkrgilaeifkaLaeeeinvdlisqtese...tsislvvdeedvdeakkaLkeesgaaelesle 338
                                                 ++ +++gi+a++f++La+++in+d+i+q  s    t+is+++ + d+++a ++L+ + +a++ e + 
  lcl|NCBI__GCF_000969705.1:WP_052664755.1 270 --KVSDQPGIAAKLFSTLADAKINIDMIVQNVSAdgrTDISFTLPRGDTNQARAILEPYAEAIGAEGVL 336
                                               ..****************************9876677******************************** PP

                                 TIGR00656 339 veedlavvsivgaglveapGvaseifkaleekninilmisssetkisvlvdekdaekavrklheklee 406
                                               v+e++a+vs+vgag++ +pGva+++f+al+  ++ni mis+s +++sv++ + ++e av ++he++ +
  lcl|NCBI__GCF_000969705.1:WP_052664755.1 337 VDENVAKVSLVGAGMKTHPGVAARMFSALSAAGVNIEMISTSTIRVSVVIAKDQVEPAVLAVHEAFGL 404
                                               ****************************************************************9965 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (407 nodes)
Target sequences:                          1  (415 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 12.55
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis