Align Putative acetolactate synthase large subunit IlvX; ALS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; AHAS (uncharacterized)
to candidate WP_052667645.1 NITAL_RS18575 acetolactate synthase large subunit
Query= curated2:O53554 (515 letters) >NCBI__GCF_000969705.1:WP_052667645.1 Length = 519 Score = 605 bits (1560), Expect = e-177 Identities = 326/519 (62%), Positives = 377/519 (72%), Gaps = 7/519 (1%) Query: 1 MNGAQALINTLVDGGVDVCFANPGTSEMHFVAALDAVPRMRGMLTLFEGVATGAADGYAR 60 MNGA ALI TLV VDVCF NPGTSEMHFVAALD VP MRG+L LFEGVATGAADGYAR Sbjct: 1 MNGANALIRTLVGADVDVCFTNPGTSEMHFVAALDDVPEMRGVLALFEGVATGAADGYAR 60 Query: 61 IAGRPAAVLLHLGPGLGNGLANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDAVAG 120 IAGRPAAVLLHLGPGLGNGLANLHNARRA P+V +VGDHA HK+ DAPLESDI+ VA Sbjct: 61 IAGRPAAVLLHLGPGLGNGLANLHNARRAATPVVNIVGDHALPHKQLDAPLESDIETVAR 120 Query: 121 TVSGWVRRTEAAADVGAD-AEAAIAASRSGSQIATLILPADVCWSDGAHAAAGV-PAQAA 178 VS WVR + + ADVG D AEA AA S Q+ATLILPADV W DG A V PA Sbjct: 121 NVSSWVRTSTSTADVGRDAAEAVAAAIGSPGQVATLILPADVSWGDGGVVADPVAPAGRR 180 Query: 179 AAPVD-VGPVAGVLRSGEPAMMLIGGDATRGP-GLTAAARIVQATGARWLCETFPTCLER 236 P D + A LRSGE ++L+GGDA P + AAA I QA+GA L E FPT L R Sbjct: 181 GVPADTITGAAKALRSGEATLILVGGDALADPTAVDAAATIAQASGAAILAEVFPTRLAR 240 Query: 237 GAGIPAVERLAYFAEGAAAQLDGVKHLVLAGARSPVSFFAYPGMPSDLVPAGCEVHVLAE 296 G G+ ++RL Y +E A+ QL GV+HL+L GA PV+FFAYP PS LVP G EVH+LA Sbjct: 241 GQGVAPLDRLGYLSEAASQQLAGVRHLILVGAEEPVTFFAYPDKPSRLVPEGTEVHMLAG 300 Query: 297 PGGAA-DALAALADEVAPGTVAPVAGASRP-QLPTGDLTSVSAADVVGALLPERAIVVDE 354 AA DALAALADE+ VAP A P Q PTG L + +A+ VGALLPE AIVVDE Sbjct: 301 ASDAAGDALAALADELGCAGVAPPRPAGEPSQRPTGQLDAWTASLAVGALLPEGAIVVDE 360 Query: 355 SNTCGVLLPQATAGAPAHDWLTLTGGAIGYGIPAAVGAAVAAPDRPVLCLESDGSAMYTI 414 + T + LP TA +P HDWLTLTGG+IGYG+PAAVGAA+AAPDR VL L++DGSAMYTI Sbjct: 361 AVTSSLSLPMTTAASPRHDWLTLTGGSIGYGLPAAVGAAIAAPDRKVLALQADGSAMYTI 420 Query: 415 SGLWSQARENLDVTTVIYNNGAYDILRIELQRVGAGSDPGPKALDLLDISRPTMDFVKIA 474 LW+ ARE LDVT V+YNN +Y+IL++ELQRVGA + GP A DL D+ RP +DF ++A Sbjct: 421 QSLWTMAREELDVTVVLYNNASYEILKMELQRVGADT-TGPAAADLFDLGRPRLDFTELA 479 Query: 475 EGMGVPARRVTTCEEFADALRAAFAEPGPHLIDVVVPSL 513 GMGVPA R TT +EF L AA A PGP L++ ++P L Sbjct: 480 RGMGVPATRATTADEFTVQLEAALATPGPSLVEAMIPKL 518 Lambda K H 0.318 0.134 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 724 Number of extensions: 39 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 515 Length of database: 519 Length adjustment: 35 Effective length of query: 480 Effective length of database: 484 Effective search space: 232320 Effective search space used: 232320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory