Alignments for a candidate for prpE in Stenotrophomonas chelatiphaga DSM 21508

GapMind for Amino acid biosynthesis

Alignments for a candidate for prpE in Stenotrophomonas chelatiphaga DSM 21508

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate WP_057507083.1 ABB28_RS02385 acetate--CoA ligase

Query= SwissProt::P27550
         (652 letters)



>NCBI__GCF_001431535.1:WP_057507083.1
          Length = 647

 Score =  852 bits (2201), Expect = 0.0
 Identities = 397/641 (61%), Positives = 497/641 (77%), Gaps = 1/641 (0%)

Query: 7   HTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGKILDWIKPYQKVKNTSFAPGNVS 66
           + +    A    I+   Y+  YQ SI+ PD FWGE  + L W K    +KN S+   +  
Sbjct: 5   YPVDPQFAKNARIDKTTYQQQYQASIDAPDAFWGEAAQRLQWFKQPSIIKNVSYDLADFR 64

Query: 67  IKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA-SQSKHISYKELHRDVCRFANTLL 125
           IKW+EDG LN + NCLDR L++ G++TA+++E D   + ++H++Y+EL+   CR  N L 
Sbjct: 65  IKWFEDGELNASVNCLDRQLEKRGEKTALLFEPDSPDAPAQHVTYRELYERTCRLGNALR 124

Query: 126 ELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSNSRLVIT 185
            LG+KKGD V IY+PM+ +AAVAMLACARIGA+HSV+FGGF+P ++A R+ D  S+L+IT
Sbjct: 125 NLGVKKGDRVTIYLPMIVDAAVAMLACARIGAIHSVVFGGFAPNSIADRVSDCASKLIIT 184

Query: 186 SDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVVVLKRTGGKIDWQEGRDLWWHDLVEQAS 245
           +DEG+R G+ IPLK NVD ALK P   +VE V+V++ TGG +D Q  RD W+HD+V+   
Sbjct: 185 ADEGLRGGKKIPLKANVDAALKLPGTNTVETVLVVRHTGGAVDMQAPRDRWFHDVVDSQP 244

Query: 246 DQHQAEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDYHPGDIYWCTA 305
            + + E MNAEDPLFILYTSGSTGKPKGVLHTTGGYL+YAA T + VFD    DIYWCTA
Sbjct: 245 AECEPERMNAEDPLFILYTSGSTGKPKGVLHTTGGYLLYAAYTHEAVFDLREDDIYWCTA 304

Query: 306 DVGWVTGHSYLLYGPLACGATTLMFEGVPNWPTPARMAQVVDKHQVNILYTAPTAIRALM 365
           DVGWVTGHSY++YGPLA GAT+LMFEGVPN+P  +R   V+DKH+V++ YTAPTAIRALM
Sbjct: 305 DVGWVTGHSYIVYGPLANGATSLMFEGVPNYPDTSRFWNVIDKHKVSLFYTAPTAIRALM 364

Query: 366 AEGDKAIEGTDRSSLRILGSVGEPINPEAWEWYWKKIGNEKCPVVDTWWQTETGGFMITP 425
            EG++ ++ T R+SLR+LGSVGEPINPEAW WY++ +G+ +CP+VDTWWQTETGG +I+P
Sbjct: 365 REGEEPVKRTSRASLRLLGSVGEPINPEAWRWYYEVVGDSRCPIVDTWWQTETGGILISP 424

Query: 426 LPGATELKAGSATRPFFGVQPALVDNEGNPLEGATEGSLVITDSWPGQARTLFGDHERFE 485
           LPGA +LK GSA+ PFFGVQPALV+ +G   +GATEG+L+I DSWPGQ RT++GD +RF 
Sbjct: 425 LPGAVDLKPGSASLPFFGVQPALVNADGEIKDGATEGNLIIRDSWPGQMRTVYGDDQRFI 484

Query: 486 QTYFSTFKNMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEA 545
            TYF T+   YF+GDG RRDEDGYYWITGRVDDV+NVSGHR+GTAE+ESALV+HPK+AEA
Sbjct: 485 DTYFRTYPGAYFTGDGCRRDEDGYYWITGRVDDVINVSGHRIGTAEVESALVSHPKVAEA 544

Query: 546 AVVGIPHNIKGQAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKT 605
           AVVG PH +KGQ IYAYVTL   E PS EL+ E+  WVRKEIGP+ATPD L W   LPKT
Sbjct: 545 AVVGFPHEVKGQGIYAYVTLIADEAPSEELHKELVAWVRKEIGPIATPDHLQWAPGLPKT 604

Query: 606 RSGKIMRRILRKIAAGDTSNLGDTSTLADPGVVEKLLEEKQ 646
           RSGKIMRRILRKIA      LGDTSTLADP VV  L++E++
Sbjct: 605 RSGKIMRRILRKIAENAPDQLGDTSTLADPSVVASLVDERK 645


Lambda     K      H
   0.317    0.135    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1387
Number of extensions: 63
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 652
Length of database: 647
Length adjustment: 38
Effective length of query: 614
Effective length of database: 609
Effective search space:   373926
Effective search space used:   373926
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis