Alignments for a candidate for hcs in Stenotrophomonas chelatiphaga DSM 21508

GapMind for Amino acid biosynthesis

Alignments for a candidate for hcs in Stenotrophomonas chelatiphaga DSM 21508

Align homocitrate synthase (EC 2.3.3.14) (characterized)
to candidate WP_057509301.1 ABB28_RS14490 2-isopropylmalate synthase

Query= BRENDA::D0VY45
         (540 letters)



>NCBI__GCF_001431535.1:WP_057509301.1
          Length = 527

 Score =  388 bits (997), Expect = e-112
 Identities = 226/522 (43%), Positives = 323/522 (61%), Gaps = 27/522 (5%)

Query: 22  PTYVRILDTTLRDGEQSPGAAMTCVQKLETARQLAKLGVDIIEAGFPCASKQDFMAVKMI 81
           P  +RI DTTLRDGEQSPG +M+  QKL  AR L +LGVD+IE GFP +S+ D  A+ MI
Sbjct: 11  PPRIRIFDTTLRDGEQSPGCSMSPQQKLVMARALDELGVDVIETGFPASSQSDREAMTMI 70

Query: 82  AEEVGNCVDGNGYVPVITGVSRCNEKDIATAWEALKHAKRPRLRTFIATSPIHMEYKLRK 141
             EV          PV+  +SRC   DI T+  +L  A  PRL  F++TSP+H E+KLR 
Sbjct: 71  GREVQR--------PVLAVLSRCLAADIETSVRSLDSAAHPRLHVFLSTSPLHREHKLRM 122

Query: 142 SKDQVLETARNMVKFARSLGCTDIQFGAEDAARSDKEFLYQIFGEVIKAGATTLTIPDTV 201
           +++QVLE+ R  V  ARS    D++F AEDA R++ ++L ++    I AGATT+ +PDTV
Sbjct: 123 TREQVLESVRKHVALARSY-IDDVEFSAEDATRTELDYLIEVSRVAIAAGATTINLPDTV 181

Query: 202 GIAMPFE----YGKLIADIKANTPGIENAIMATHCHNDLGLATANTIEGARYGARQLEVT 257
           G   P E    + ++IA + A+  G E  I + HCHNDLGLA AN++     GARQ+E T
Sbjct: 182 GFTTPEEIRAMFQQVIAGV-ADVSGAERVIFSAHCHNDLGLAVANSLAAIEGGARQVECT 240

Query: 258 INGIGERAGNASFEEVVMALTCRGIDILGGLHTGINTRHILKTSKMVEKYSGLHLQPHKA 317
           +NGIGERAGN + EE+ MAL  R         T INT+ I+ TS+++++  G+ +Q +KA
Sbjct: 241 VNGIGERAGNCAVEEIAMALKVR--QAFYDQDTAINTQRIVSTSQLLQRLVGMPVQRNKA 298

Query: 318 LVGANAFLHESGIHQDGMLKHRGTYEIISPEDIGLVRSVGDTIVLGKLSGRQALRNRLEE 377
           +VGANAF HESGIHQ GML+HRGTYEI+ PED+G   S    +VLG+ SGR A+  RL  
Sbjct: 299 IVGANAFAHESGIHQHGMLRHRGTYEIMRPEDVGWEDS---QMVLGRHSGRAAVEARLRA 355

Query: 378 LGYKLKDTEVEGVFWQFKAVAEKKKRITDTDLRALVSNEAFNEQPIWKLGDLQVTCGTVG 437
           LG+ L++ E++ VF QFK++ E+++ +TD+DL+ L+   A  +   ++L  +  T   VG
Sbjct: 356 LGFWLEEEELKLVFEQFKSLCEQQRVVTDSDLQTLMQGGASTQG--YRLASM--TISDVG 411

Query: 438 F-STATVKLFSIDGSMHVACSIGTGPVDSAYKAINHIVKEPAKLVKYTLGAITEGIDATA 496
             + A V+L   DG+     + G GPVD+ + A++       +L  Y + ++  G DA  
Sbjct: 412 SRANALVELSDPDGNRVAETAQGDGPVDALFGALSSATGVSLQLDSYQVHSVGIGADARG 471

Query: 497 TTSVEISRGDTNHPVFSGTGGGTDVVVSSVDAYLSALNNMLR 538
             ++ +  GD    VF GTG   D++ +S  A+L   N +LR
Sbjct: 472 EANLSVRSGD---EVFEGTGTSRDIIEASALAWLDVANRLLR 510


Lambda     K      H
   0.318    0.134    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 605
Number of extensions: 25
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 540
Length of database: 527
Length adjustment: 35
Effective length of query: 505
Effective length of database: 492
Effective search space:   248460
Effective search space used:   248460
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis