Alignments for a candidate for trpD_2 in Stenotrophomonas chelatiphaga DSM 21508

GapMind for Amino acid biosynthesis

Alignments for a candidate for trpD_2 in Stenotrophomonas chelatiphaga DSM 21508

Align Anthranilate phosphoribosyltransferase; EC 2.4.2.18 (characterized)
to candidate WP_057509110.1 ABB28_RS13455 anthranilate phosphoribosyltransferase

Query= SwissProt::Q8PD71
         (345 letters)



>NCBI__GCF_001431535.1:WP_057509110.1
          Length = 343

 Score =  527 bits (1357), Expect = e-154
 Identities = 268/338 (79%), Positives = 295/338 (87%)

Query: 1   MPITPQQALQRTIEHREIFHDEMVDLMRQIMRGEVSDAMVSAILTGLRVKKETIGEIAGA 60
           M  +PQ+ALQRTIEHREIF +EMVDLMRQIMRG+VS  M +AILTGLRVKKET+ EIA A
Sbjct: 1   MRFSPQEALQRTIEHREIFFEEMVDLMRQIMRGDVSPMMTAAILTGLRVKKETVDEIAAA 60

Query: 61  ATVMREFSRRVEVTDRRHMVDIVGTGGDGSHTFNISTCAMFVAAAGGAKVAKHGNRSVSS 120
           ATVMREF+  V V D RHMVDIVGTGGDGSHTFNISTC+MFVAAA GA+VAKHGNRSVSS
Sbjct: 61  ATVMREFALPVPVADTRHMVDIVGTGGDGSHTFNISTCSMFVAAAAGARVAKHGNRSVSS 120

Query: 121 KSGSADALEALGAVIELQPEQVAASLAQTGIGFMYAPVHHPAMKVVAPVRREMGVRTIFN 180
           KSGSADA+EALGAVIELQP QVA ++ QTG+GFM+AP+HHPAMKVVAPVRREMGVRTIFN
Sbjct: 121 KSGSADAVEALGAVIELQPAQVAQAIEQTGVGFMFAPIHHPAMKVVAPVRREMGVRTIFN 180

Query: 181 ILGPLTNPAGSPNILMGVFHPDLVGIQARVLQELGAERALVVWGRDGMDELSLGAGTLVG 240
           ILGPLTNPA +P++LMGVFHPDLVGIQARVL+EL   RA+VVWGRD MDE+SLGAGTLVG
Sbjct: 181 ILGPLTNPASAPSVLMGVFHPDLVGIQARVLRELDTRRAMVVWGRDNMDEISLGAGTLVG 240

Query: 241 ELRDGQVHEYEVHPEDFGIAMSASRNLKVADAAESRAMLLQVLDNVPGPALDIVALNAGA 300
           ELRDG+V EYE+HPEDFGIAMSASRNL+V    +S AML  VLDN PGPALDIVALNAGA
Sbjct: 241 ELRDGKVREYEIHPEDFGIAMSASRNLRVDGPEQSIAMLRAVLDNEPGPALDIVALNAGA 300

Query: 301 ALYVAGVADSIADGIVRARQVLADGSARACLDAYVAFT 338
           ALYVA VA  I DG+ RAR  +ADGSARA L  YV  T
Sbjct: 301 ALYVADVASDIGDGLARARAAIADGSARARLQQYVDIT 338


Lambda     K      H
   0.320    0.134    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 480
Number of extensions: 14
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 345
Length of database: 343
Length adjustment: 29
Effective length of query: 316
Effective length of database: 314
Effective search space:    99224
Effective search space used:    99224
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_057509110.1 ABB28_RS13455 (anthranilate phosphoribosyltransferase)
to HMM TIGR01245 (trpD: anthranilate phosphoribosyltransferase (EC 2.4.2.18))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01245.hmm
# target sequence database:        /tmp/gapView.17159.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01245  [M=330]
Accession:   TIGR01245
Description: trpD: anthranilate phosphoribosyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   4.6e-129  416.4   0.6   5.3e-129  416.2   0.6    1.0  1  lcl|NCBI__GCF_001431535.1:WP_057509110.1  ABB28_RS13455 anthranilate phosp


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_001431535.1:WP_057509110.1  ABB28_RS13455 anthranilate phosphoribosyltransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  416.2   0.6  5.3e-129  5.3e-129       1     329 [.       9     336 ..       9     337 .. 0.99

  Alignments for each domain:
  == domain 1  score: 416.2 bits;  conditional E-value: 5.3e-129
                                 TIGR01245   1 leklldnkdLseeeaeqlmkeimsgeasdaqiaAilvalrvkgeteeeiaglakalrekakkvekeese 69 
                                               l++ ++++++  ee+ +lm++im+g++s++  aAil+ lrvk et++eia++a+++re a  v++ +++
  lcl|NCBI__GCF_001431535.1:WP_057509110.1   9 LQRTIEHREIFFEEMVDLMRQIMRGDVSPMMTAAILTGLRVKKETVDEIAAAATVMREFALPVPVADTR 77 
                                               5788999************************************************************** PP

                                 TIGR01245  70 elvDivGTGGDglktiNiSTasalvaaaaGvkvaKhGnrsvssksGsaDvLealgvnlelspekvarsl 138
                                               ++vDivGTGGDg++t+NiST s++vaaaaG++vaKhGnrsvssksGsaD +ealg+ +el+p++va+++
  lcl|NCBI__GCF_001431535.1:WP_057509110.1  78 HMVDIVGTGGDGSHTFNISTCSMFVAAAAGARVAKHGNRSVSSKSGSADAVEALGAVIELQPAQVAQAI 146
                                               ********************************************************************* PP

                                 TIGR01245 139 eevgigFlfAPkyhpalkevapvRkeLgvrtvfNlLGPLlnParaklqvlGvyskdlvevlaevlknlg 207
                                               e++g+gF+fAP +hpa+k vapvR+e+gvrt+fN+LGPL+nPa+a+  ++Gv+++dlv + a+vl++l 
  lcl|NCBI__GCF_001431535.1:WP_057509110.1 147 EQTGVGFMFAPIHHPAMKVVAPVRREMGVRTIFNILGPLTNPASAPSVLMGVFHPDLVGIQARVLRELD 215
                                               ********************************************************************* PP

                                 TIGR01245 208 vkralvvhgedglDEisltgetkvaelkdgeieeytlspedfglkraeleelkggsaeenaellkevle 276
                                               ++ra+vv g+d++DEisl + t v el+dg+++ey+++pedfg+++   ++l+++ +e++ ++l++vl 
  lcl|NCBI__GCF_001431535.1:WP_057509110.1 216 TRRAMVVWGRDNMDEISLGAGTLVGELRDGKVREYEIHPEDFGIAMSASRNLRVDGPEQSIAMLRAVLD 284
                                               *****************************************************************9999 PP

                                 TIGR01245 277 gkekkakrdivvlNaaaalyvagkakdlkegvelakeaiksgkalekleelva 329
                                               ++  +++ div+lNa+aalyva++a+d+ +g+ +a++ai +g+a + l+++v+
  lcl|NCBI__GCF_001431535.1:WP_057509110.1 285 NEP-GPALDIVALNAGAALYVADVASDIGDGLARARAAIADGSARARLQQYVD 336
                                               998.999*******************************************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (330 nodes)
Target sequences:                          1  (343 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 7.60
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis