Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate WP_058929394.1 AU252_RS02595 3-deoxy-7-phosphoheptulonate synthase class II
Query= BRENDA::O68903 (455 letters) >NCBI__GCF_001484605.1:WP_058929394.1 Length = 464 Score = 504 bits (1298), Expect = e-147 Identities = 250/438 (57%), Positives = 314/438 (71%), Gaps = 6/438 (1%) Query: 19 LDDALGRPAAQQPEWPDADLARRVRAVLESVPPITVPAEIDRLRENLAAVARGEAFMLQG 78 LDD P +QQP W D D+ L +PP+ E+D LRE LAA A G+AF+LQG Sbjct: 27 LDDWRDLPVSQQPSWQDKDVFDASLGELSVLPPLVFAGEVDVLRERLAAAAEGKAFLLQG 86 Query: 79 GDCAETFADNTEPHIRANVRTLLQMAVVLTYGASLPVVKIGRIAGQYAKPRSSGT---DA 135 GDCAETF T I A V+T+LQMAVVLTYGA++PV+K+GR+AGQ+AKPRSS D Sbjct: 87 GDCAETFEAATADKISARVKTILQMAVVLTYGAAMPVIKMGRMAGQFAKPRSSNDETRDG 146 Query: 136 LGLPSYRGDIVNSLVPTPEARVADPGRMIRAYANAGAAMNLLRAMTTAGMADLHRLHDWN 195 + LP+YRGDIVN TPE+R D RM++AY + + +NL+RA T G ADL +H WN Sbjct: 147 VTLPAYRGDIVNGYEFTPESRGHDAARMLKAYHTSASTLNLIRAFTQGGFADLRSVHQWN 206 Query: 196 KDFVRTSPAGERYEALAAEIDRGLRFMSACGVNDSSLHTTEIFASHEALLLDYERALLRL 255 K F +PA RYE+LA +IDR ++FM++CG + +L E FASHEALLLDYERAL R+ Sbjct: 207 KGFTE-NPAHARYESLARDIDRAIKFMASCGADFEALKRVEFFASHEALLLDYERALTRI 265 Query: 256 DTRGDEPKLYDLSSHFLWIGERTKQLDGAHIAFAELLANPIGLKIGPSTTPEMAVEYVER 315 D+R P YD S+HFLWIGERT++LD AH+ F + NPIG+K+GPSTT + A+ +++ Sbjct: 266 DSRTGFP--YDTSAHFLWIGERTRELDHAHVDFLSRVRNPIGVKLGPSTTGDDALRLIDK 323 Query: 316 LDPHNQPGRLTLIIRMGNGKVRDVLPAIVEKVTASGHKVIWQCDPMHGNTHESSTGYKTR 375 LDP +PGRLT I RMG G +R+ LPAIVEKVTASG KV+W DPMHGNT S GYKTR Sbjct: 324 LDPEREPGRLTFITRMGAGNIREKLPAIVEKVTASGAKVLWVTDPMHGNTVTSPNGYKTR 383 Query: 376 HFDRIVDEVQGFFEVHRRLGTHPGGIHIELTGEDVTECLGGAQEISDTDLAGRYETACDP 435 +FD ++DEV+GFFEVH LGT PGG+H+E+TG+DV ECLGGA + +YE+ CDP Sbjct: 384 NFDDVIDEVRGFFEVHHALGTVPGGLHVEMTGDDVAECLGGADPVDQEAFLDKYESVCDP 443 Query: 436 RLNTQQSLELAFLVAEML 453 RLN QSLE+AFLVA L Sbjct: 444 RLNHMQSLEMAFLVAGAL 461 Lambda K H 0.319 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 608 Number of extensions: 17 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 455 Length of database: 464 Length adjustment: 33 Effective length of query: 422 Effective length of database: 431 Effective search space: 181882 Effective search space used: 181882 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
Align candidate WP_058929394.1 AU252_RS02595 (3-deoxy-7-phosphoheptulonate synthase class II)
to HMM TIGR01358 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01358.hmm # target sequence database: /tmp/gapView.27605.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01358 [M=443] Accession: TIGR01358 Description: DAHP_synth_II: 3-deoxy-7-phosphoheptulonate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.2e-207 673.9 0.0 5.2e-207 673.6 0.0 1.0 1 lcl|NCBI__GCF_001484605.1:WP_058929394.1 AU252_RS02595 3-deoxy-7-phosphoh Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_001484605.1:WP_058929394.1 AU252_RS02595 3-deoxy-7-phosphoheptulonate synthase class II # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 673.6 0.0 5.2e-207 5.2e-207 4 439 .. 28 461 .. 26 464 .] 0.99 Alignments for each domain: == domain 1 score: 673.6 bits; conditional E-value: 5.2e-207 TIGR01358 4 eswrskpaaqlPeyPdaealeavldtleslPPlvlageilklkeklaevakGeafllqgGdcaesfkev 72 + wr+ p q+P + d++ +a l +l+ lPPlv+age++ l+e+la++a+G+afllqgGdcae+f++ lcl|NCBI__GCF_001484605.1:WP_058929394.1 28 DDWRDLPVSQQPSWQDKDVFDASLGELSVLPPLVFAGEVDVLRERLAAAAEGKAFLLQGGDCAETFEAA 96 78******************************************************************* PP TIGR01358 73 eadnirdklrvllqmavvltygaslPvvkvgriaGqyakPrsepieakdgvtlpsyrGdvingaafdea 141 +ad+i+ +++++lqmavvltyga +Pv+k+gr+aGq+akPrs++ e++dgvtlp+yrGd++ng +f+ + lcl|NCBI__GCF_001484605.1:WP_058929394.1 97 TADKISARVKTILQMAVVLTYGAAMPVIKMGRMAGQFAKPRSSNDETRDGVTLPAYRGDIVNGYEFTPE 165 ********************************************************************* PP TIGR01358 142 arvpdperlvrayaksaatlnllraltsgGyadlkkvheWnlefvkkspagaryeklaeeidealrfms 210 +r +d+ r+++ay++sa+tlnl+ra+t+gG+adl+ vh+Wn+ f++ pa arye la++id+a++fm lcl|NCBI__GCF_001484605.1:WP_058929394.1 166 SRGHDAARMLKAYHTSASTLNLIRAFTQGGFADLRSVHQWNKGFTE-NPAHARYESLARDIDRAIKFMA 233 ********************************************86.6999****************** PP TIGR01358 211 avgvaeaealkrvelytsheallldyeealtrvdskegqlfdlsahllWiGertrqldgahveflrgvk 279 ++g + + lkrve+++sheallldye+altr ds++g +d+sah+lWiGertr+ld+ahv+fl++v+ lcl|NCBI__GCF_001484605.1:WP_058929394.1 234 SCGADFEA-LKRVEFFASHEALLLDYERALTRIDSRTGFPYDTSAHFLWIGERTRELDHAHVDFLSRVR 301 ***98877.************************************************************ PP TIGR01358 280 nPigikvgpsmeadellklievldPenePGrltlisrlGaekiaeklPelleavkaaGravvWvtdpmh 348 nPig+k+gps+++d++l+li++ldPe+ePGrlt+i+r+Ga +i+eklP ++e+v+a+G++v+Wvtdpmh lcl|NCBI__GCF_001484605.1:WP_058929394.1 302 NPIGVKLGPSTTGDDALRLIDKLDPEREPGRLTFITRMGAGNIREKLPAIVEKVTASGAKVLWVTDPMH 370 ********************************************************************* PP TIGR01358 349 GntleaasGyktrrfddilsevkeffevhkaeGthpGGvhleltGedvteclGGareitetdlasryet 417 Gnt+++++Gyktr+fdd+++ev++ffevh+a+Gt pGG+h+e+tG+dv+eclGGa+ + ++ + ++ye+ lcl|NCBI__GCF_001484605.1:WP_058929394.1 371 GNTVTSPNGYKTRNFDDVIDEVRGFFEVHHALGTVPGGLHVEMTGDDVAECLGGADPVDQEAFLDKYES 439 ********************************************************************* PP TIGR01358 418 acdPrlnaeqslelaflvaekl 439 cdPrln+ qsle+aflva l lcl|NCBI__GCF_001484605.1:WP_058929394.1 440 VCDPRLNHMQSLEMAFLVAGAL 461 ******************9766 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (443 nodes) Target sequences: 1 (464 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 7.60 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory