Alignments for a candidate for thrB in Pseudarthrobacter sulfonivorans Ar51

GapMind for Amino acid biosynthesis

Alignments for a candidate for thrB in Pseudarthrobacter sulfonivorans Ar51

Align homoserine kinase (EC 2.7.1.39) (characterized)
to candidate WP_058932002.1 AU252_RS18660 homoserine kinase

Query= BRENDA::P07128
         (309 letters)



>NCBI__GCF_001484605.1:WP_058932002.1
          Length = 324

 Score =  218 bits (556), Expect = 1e-61
 Identities = 121/273 (44%), Positives = 167/273 (61%), Gaps = 10/273 (3%)

Query: 5   LNVGRKVTVTVPGSSANLGPGFDTLGLALSVYDTVEVEIIPSG-LEVEVFGEGQGEVPLD 63
           +  G++VTV VP +SANLGPG+D+LGLAL+++DT+ VE + S  L  E+ GEG   +P D
Sbjct: 14  IEAGQRVTVRVPATSANLGPGYDSLGLALALHDTLTVESLESAELVFELSGEGADTLPQD 73

Query: 64  GSHLVVKAIRAGLKAADAEVPGLRVVCHNNIPQSRGLGSSAAAAVAGVAAANGLADFPLT 123
            SHLVV+A+ A          GLR+   N  P  RGLGSSA+A VA V+AAN +      
Sbjct: 74  ASHLVVRAMEAAFSRLGYRHGGLRITATNVNPHGRGLGSSASAVVAAVSAANAMVPEAAR 133

Query: 124 Q--EQIVQLSSAFEGHPDNAAASVLGGAVVSWTNLSIDGKSQPQYAAVPLEVQDNIRATA 181
           +  + I+QL+S  EGHPDN A ++ GG  +SW       +   +Y++    V  ++    
Sbjct: 134 RGRDWILQLTSEMEGHPDNVAPAIFGGLALSW-------QDSDRYSSTCATVAGSVIPIV 186

Query: 182 LVPNFHASTEAVRRVLPTEVTHIDARFNVSRVAVMIVALQQRPDLLWEGTRDRLHQPYRA 241
            VP+F  STEA R +LP  V H  A  N  R A++I AL Q+P+ L  GT D LHQ YRA
Sbjct: 187 AVPDFELSTEAARALLPASVGHHAAAMNSGRAALLIHALTQKPEFLHAGTEDYLHQSYRA 246

Query: 242 EVLPITSEWVNRLRNRGYAAYLSGAGPTAMVLS 274
           E +P ++  +  LRN G+AA +SGAGPT +VL+
Sbjct: 247 EAMPPSASLIRALRNSGHAAVVSGAGPTVLVLA 279


Lambda     K      H
   0.315    0.132    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 229
Number of extensions: 15
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 309
Length of database: 324
Length adjustment: 27
Effective length of query: 282
Effective length of database: 297
Effective search space:    83754
Effective search space used:    83754
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 48 (23.1 bits)

Align candidate WP_058932002.1 AU252_RS18660 (homoserine kinase)
to HMM TIGR00191 (thrB: homoserine kinase (EC 2.7.1.39))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00191.hmm
# target sequence database:        /tmp/gapView.7609.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00191  [M=304]
Accession:   TIGR00191
Description: thrB: homoserine kinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    2.3e-69  219.5   0.0    2.7e-69  219.3   0.0    1.0  1  lcl|NCBI__GCF_001484605.1:WP_058932002.1  AU252_RS18660 homoserine kinase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_001484605.1:WP_058932002.1  AU252_RS18660 homoserine kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  219.3   0.0   2.7e-69   2.7e-69       1     301 [.      20     319 ..      20     321 .. 0.90

  Alignments for each domain:
  == domain 1  score: 219.3 bits;  conditional E-value: 2.7e-69
                                 TIGR00191   1 lkvkvPassANlgpGfDvlGlalslvlellvtedvaqeskdksleaegegvekipkesdkNliyqvakk 69 
                                               ++v+vPa+sANlgpG+D+lGlal l + l+v+     es++  +e  geg++++p++    l+ ++++ 
  lcl|NCBI__GCF_001484605.1:WP_058932002.1  20 VTVRVPATSANLGPGYDSLGLALALHDTLTVES---LESAELVFELSGEGADTLPQD-ASHLVVRAMEA 84 
                                               689****************************99...8888888**************.899******** PP

                                 TIGR00191  70 vlkklgkrvkpvkltvekeiplgrGLGSSaaaivaaviaanelaglklskee..lldlalllEgHpDNv 136
                                                + +lg r  ++++t ++  p grGLGSSa+a+vaav aan+++ +   + +  +l+l +++EgHpDNv
  lcl|NCBI__GCF_001484605.1:WP_058932002.1  85 AFSRLGYRHGGLRITATNVNPHGRGLGSSASAVVAAVSAANAMVPEAARRGRdwILQLTSEMEGHPDNV 153
                                               ********************************************99887654449************** PP

                                 TIGR00191 137 apallGGlqlavkeddllevlkvPsgsklkvvlviPnievsTaeaRavLPkaysrqdlvfnlshlavlv 205
                                               apa++GGl l+ ++ d   ++      ++  ++++P +e+sT++aRa+LP+++  +++ +n+ ++a+l+
  lcl|NCBI__GCF_001484605.1:WP_058932002.1 154 APAIFGGLALSWQDSDRYSSTCATVAGSVIPIVAVPDFELSTEAARALLPASVGHHAAAMNSGRAALLI 222
                                               ****************6666666666899999************************************* PP

                                 TIGR00191 206 tAlvskdkadllaiamkDrvhqpyRekliPelteikqaakekgalgitlSGaGptilalaeeek.eeka 273
                                               +Al++k  +++l+   +D +hq yR++ +P  + + +a ++ g  + ++SGaGpt+l+la+ e  +  +
  lcl|NCBI__GCF_001484605.1:WP_058932002.1 223 HALTQK--PEFLHAGTEDYLHQSYRAEAMPPSASLIRALRNSG-HAAVVSGAGPTVLVLADGEAeAAEV 288
                                               *****9..9**********************999999988886.57789************99867777 PP

                                 TIGR00191 274 qelleklakegi.eltvkvle..ldtdgaev 301
                                                  +e+++++++  + ++vl+  +d +ga+v
  lcl|NCBI__GCF_001484605.1:WP_058932002.1 289 LGFIESFTEANTpGIGWRVLKlaVDVEGAKV 319
                                               7888888776541445665541156677765 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (304 nodes)
Target sequences:                          1  (324 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 12.22
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis