Align aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) (characterized)
to candidate WP_068460798.1 APY04_RS06185 aspartate--tRNA ligase
Query= reanno::Caulo:CCNA_01969 (612 letters) >NCBI__GCF_001541235.1:WP_068460798.1 Length = 601 Score = 785 bits (2027), Expect = 0.0 Identities = 392/615 (63%), Positives = 472/615 (76%), Gaps = 24/615 (3%) Query: 4 MHAYRTHNCGALRASDTGAAVRLSGWIHRKRDHGGLVFIDLRDHYGLTQLVLHPETPGFN 63 MH YR+H CGALRASD G VRLSGW+HR RDHGG++FIDLRDHYG+TQ+V P++P F Sbjct: 1 MHRYRSHTCGALRASDIGNTVRLSGWVHRVRDHGGVLFIDLRDHYGITQVVADPDSPAFK 60 Query: 64 VVERLRAESVIRVDGEVIARDASVVNPNLPTGEIEIRVSAVEVLSEAAELPLPVFGEPDY 123 E +R+E VIR+DG V R N +LPTGEIE+ + +EVLS+A ELP+PVFGEPDY Sbjct: 61 NAEAVRSEWVIRIDGTVRKRPDGTTNADLPTGEIELFATEIEVLSQAKELPVPVFGEPDY 120 Query: 124 PEEIRLKHRYLDLRRETLHKNIVLRSRVIQSIRNRMFAQGFNEFQTPILTASSPEGARDF 183 PE++RL++R+LDLRRETLH+NI+ R +I S+R RM GF EF TPILTASSPEGARDF Sbjct: 121 PEDLRLQYRFLDLRRETLHRNIMQRMAIIASLRKRMTEGGFFEFSTPILTASSPEGARDF 180 Query: 184 LVPSRLHPEKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDLRADR-SLEFYQLDVE 242 LVPSR+H KFYALPQAPQQ+KQLLMVSGFDRYFQIAPCFRDED RADR EFYQLDVE Sbjct: 181 LVPSRIHAGKFYALPQAPQQYKQLLMVSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE 240 Query: 243 MSFVTQEDVFAAIEPVMHGVFEEFSNGKPVSPINGTH-TFTNDFGQSFEHKGFERLTYAQ 301 MSFVTQ+DVF +EPV+ GVFEEF S I G+ + TN G+ R+ Y++ Sbjct: 241 MSFVTQDDVFNEMEPVITGVFEEF------SAITGSEKSVTN---------GWPRIPYSE 285 Query: 302 SMAWYGSDKPDLRNPIKMANVSEHFRDGGFGLFAKILGADAKNQVWAIPAPTGGSRAFCD 361 ++A YGSDKPDLRNPI+M +V+EHFR GF +FA ++ D K +VWAIPAP GGSRAFCD Sbjct: 286 AIAKYGSDKPDLRNPIEMQDVTEHFRGSGFKIFAGMIDKDPKVRVWAIPAPGGGSRAFCD 345 Query: 362 RMNSWAQGEGQPGLGYVFWSEDQGGWGGPIAKNLG-EPTQALMESLGLGAGDAAFFVAGD 420 RMNSWAQGEGQPGL Y+F+ E G GP+ KNLG E A+ E +GL GDA FF AG+ Sbjct: 346 RMNSWAQGEGQPGLAYIFFRE--GEAAGPVGKNLGPERADAIREQMGLKEGDAVFFTAGN 403 Query: 421 PAVFAKFAGLARTRVGTELKLVDENQFKFCWIVDFPMFEWNEEEKKVDFSHNPFSMPQGG 480 PA F +FAG ARTR+G ELKL+DE++F WIVDFP +EWN+++KK+DFSHNPFSMPQGG Sbjct: 404 PARFHRFAGDARTRIGEELKLIDESKFALAWIVDFPFYEWNDDDKKIDFSHNPFSMPQGG 463 Query: 481 LEALE----TQDPLTIRAYQYDIVCNGYELCSGAIRNHKPEIMLKAFATAGYGPEVVEEQ 536 LEALE + L I+AYQYDIVCNG+E+ SG+IRNH+P+ M+KAF G VE + Sbjct: 464 LEALEGAASDDERLNIKAYQYDIVCNGFEIASGSIRNHRPDTMVKAFEITGLTQADVEAR 523 Query: 537 FGGMLNAFRYGAPPHGGLAPGIDRIVMLLADQVAIREVIAFPLNQQGQDLLMNAPANVLD 596 FGG+ AF+YGAPPHGG+A GIDRIVMLL +RE+ FP+NQQ DLLM AP++V Sbjct: 524 FGGLYRAFQYGAPPHGGMAAGIDRIVMLLVGAKNLREISLFPMNQQANDLLMGAPSDVAP 583 Query: 597 KQLKELHIRTAPPIK 611 KQL+ELHIR P K Sbjct: 584 KQLRELHIRVVAPEK 598 Lambda K H 0.321 0.139 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1176 Number of extensions: 47 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 612 Length of database: 601 Length adjustment: 37 Effective length of query: 575 Effective length of database: 564 Effective search space: 324300 Effective search space used: 324300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory