Align homoserine kinase (EC 2.7.1.39) (characterized)
to candidate WP_068464906.1 APY04_RS16140 homoserine kinase
Query= reanno::Korea:Ga0059261_3253 (319 letters) >NCBI__GCF_001541235.1:WP_068464906.1 Length = 324 Score = 300 bits (768), Expect = 3e-86 Identities = 151/310 (48%), Positives = 206/310 (66%), Gaps = 2/310 (0%) Query: 1 MAVYTQVSAEALAGFLARYDVGELVSAKGIAEGVENSNYLVETTRDRFILTLYEKRVEAA 60 MAVYT+VS LA F+A Y +GEL+S KGIAEGVEN+NY+V T+ FILT+YEKRVE + Sbjct: 1 MAVYTEVSDAELASFIASYGLGELLSFKGIAEGVENTNYIVHTSTGPFILTIYEKRVEVS 60 Query: 61 DLPYFMGLLDHLAAKGLPVPPAIKDRGGVEIQELNGRPACLIKFLSGISLSHPTPAQARA 120 DLP+++GL++HLA G+ P +++ G + +L+GRPA L+ FL G + PT A Sbjct: 61 DLPFYLGLMEHLAENGVTCPTPVRNAEGENLSQLSGRPAALVTFLEGFWVRQPTAAHCGH 120 Query: 121 AGEAMAQMHRAVADFPLDRPNSMGVDTWQPLFEKCGHSLDQIVPGLYDDLGFAIARVVPA 180 G A+A+MHR F + R N++G++ W+PL++K + IVP L + + + Sbjct: 121 VGRALAEMHRGGEGFAIRRANALGLNGWRPLYDKFAPDANGIVPDLAPLIEGELDYLEAN 180 Query: 181 WTRNDFDRCAIHADLFPDNVLMRGDQVTGLIDFYFACTDIRVYDLAVMHSAWSFDAHGRN 240 W R IHADLFPDNV GD+++GLIDFYFAC D YD+AV +AW F+ G Sbjct: 181 WPRG-LPEGVIHADLFPDNVFFIGDELSGLIDFYFACNDALAYDIAVCLNAWCFEKDGA- 238 Query: 241 YDPAVGDALIAGYEASFPLTEVERAAFPTLAAGACIRFSLSRAWDWLNTPADALVMRKDP 300 ++ G AL+ GYE+ PLT+ ER A PTLA GA RF L+RA+DWLNTP DALV K+P Sbjct: 239 FNITKGRALLRGYESVRPLTDAERTAMPTLARGAATRFLLTRAYDWLNTPKDALVSPKNP 298 Query: 301 LAYVRRLKHY 310 + YVR+L+ + Sbjct: 299 MEYVRKLRFH 308 Lambda K H 0.323 0.138 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 301 Number of extensions: 7 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 319 Length of database: 324 Length adjustment: 28 Effective length of query: 291 Effective length of database: 296 Effective search space: 86136 Effective search space used: 86136 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 48 (23.1 bits)
Align candidate WP_068464906.1 APY04_RS16140 (homoserine kinase)
to HMM TIGR00938 (thrB: homoserine kinase (EC 2.7.1.39))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00938.hmm # target sequence database: /tmp/gapView.23377.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00938 [M=307] Accession: TIGR00938 Description: thrB_alt: homoserine kinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.5e-124 401.2 0.0 1.8e-124 401.0 0.0 1.0 1 lcl|NCBI__GCF_001541235.1:WP_068464906.1 APY04_RS16140 homoserine kinase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_001541235.1:WP_068464906.1 APY04_RS16140 homoserine kinase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 401.0 0.0 1.8e-124 1.8e-124 1 307 [] 1 307 [. 1 307 [. 1.00 Alignments for each domain: == domain 1 score: 401.0 bits; conditional E-value: 1.8e-124 TIGR00938 1 mavytsvsdeeleafLegydlGellslkGiaeGvensnyllttdkgryvLtlyekrvkaeeLPfflell 69 mavyt+vsd el +f+ y lGells+kGiaeGven+ny++ t +g ++Lt+yekrv+ +LPf+l l+ lcl|NCBI__GCF_001541235.1:WP_068464906.1 1 MAVYTEVSDAELASFIASYGLGELLSFKGIAEGVENTNYIVHTSTGPFILTIYEKRVEVSDLPFYLGLM 69 9******************************************************************** PP TIGR00938 70 thLaerglpvakpvksrdGralseLaGkPaalvefLkGssvakPtaercrevgevlaklhlagadfkee 138 +hLae+g++++ pv++ +G++ls+L+G+Paalv fL+G v++Pta++c +vg la +h g++f + lcl|NCBI__GCF_001541235.1:WP_068464906.1 70 EHLAENGVTCPTPVRNAEGENLSQLSGRPAALVTFLEGFWVRQPTAAHCGHVGRALAEMHRGGEGFAIR 138 ********************************************************************* PP TIGR00938 139 rkndlrleaWsilaakkfkvleqleeelaalldkeldalkkflprdLPrgvihadlfkdnvlldgdklk 207 r+n l+l+ W++l k ++ + ++la l++ eld+l+ ++pr LP+gvihadlf+dnv++ gd+l+ lcl|NCBI__GCF_001541235.1:WP_068464906.1 139 RANALGLNGWRPLYDKFAPDANGIVPDLAPLIEGELDYLEANWPRGLPEGVIHADLFPDNVFFIGDELS 207 *****************999************************************************* PP TIGR00938 208 gvidfyfaCedallydlaiavndWcfeaddkldaaaakallkgyeavrpLseeekaafpvllrgaalrf 276 g+idfyfaC+dal+yd+a+++n+Wcfe+d+ +++++ +all gye+vrpL++ e+ a+p+l+rgaa rf lcl|NCBI__GCF_001541235.1:WP_068464906.1 208 GLIDFYFACNDALAYDIAVCLNAWCFEKDGAFNITKGRALLRGYESVRPLTDAERTAMPTLARGAATRF 276 ********************************************************************* PP TIGR00938 277 llsrlldlvftqagelvvakdPaeferkLkv 307 ll+r+ d+ +t+ ++lv +k+P e+ rkL++ lcl|NCBI__GCF_001541235.1:WP_068464906.1 277 LLTRAYDWLNTPKDALVSPKNPMEYVRKLRF 307 ****************************985 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (307 nodes) Target sequences: 1 (324 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 7.33 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory