Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (characterized)
to candidate WP_066922914.1 ACG33_RS06390 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA
Query= SwissProt::O06491 (485 letters) >NCBI__GCF_001579945.1:WP_066922914.1 Length = 484 Score = 488 bits (1256), Expect = e-142 Identities = 249/477 (52%), Positives = 328/477 (68%), Gaps = 1/477 (0%) Query: 8 ITELKQLIHKKEIKISDLVDESYKRIQAVDDKVQAFLALDEERARAYAKELDEAVDGRSE 67 + EL + + + +L RI+ ++ + AF+ + E A A A++ D + E Sbjct: 6 LAELGEGLRARRFSSIELARHFLDRIERLNPTLNAFITVTAETALAAAEQADRRL-AAGE 64 Query: 68 HGLLFGMPIGVKDNIVTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIGKLNMDEF 127 G L G+P+ KD T G+ TTC+S++L NF YDATVV RL A AV +GK NMDEF Sbjct: 65 EGALIGVPLAHKDIFCTDGILTTCASRMLSNFVAPYDATVVARLSRAGAVMLGKANMDEF 124 Query: 128 AMGSSTENSAYKLTKNPWNLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIRQPASFCG 187 AMGSS E S Y +NPW+LD VPGGSSGGSAAAVAA VP S G+DTGGSIRQPA+ Sbjct: 125 AMGSSNETSWYGPVRNPWDLDKVPGGSSGGSAAAVAARLVPASTGTDTGGSIRQPAALTS 184 Query: 188 VVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTSANVDVPD 247 + GLKP+YGRVSRYG++AFASSLDQ G +TR+ D A LL ++G D +DSTS + VPD Sbjct: 185 LTGLKPSYGRVSRYGMIAFASSLDQAGTLTRSAHDAALLLAQMAGFDPLDSTSVDTPVPD 244 Query: 248 FLSSLTGDIKGLKIAVPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLPHSKYALA 307 ++ L + GLKI V KE+ G+ +E V AL+V LGA EVSLP+ ++ Sbjct: 245 YVGLLAQPLTGLKIGVLKEFFERGLDEEIERRVREALEVYRRLGAQLVEVSLPNLPLSVP 304 Query: 308 TYYLLSSSEASANLARFDGIRYGYRTDNADNLIDLYKQTRAEGFGNEVKRRIMLGTFALS 367 YY+++ +E S+NL+RFDG+R+G+R D+ +L+DLYK++RAEGFG EVKRRIM GT+ LS Sbjct: 305 AYYVVAPAECSSNLSRFDGVRFGHRCDSPVDLLDLYKRSRAEGFGEEVKRRIMTGTYVLS 364 Query: 368 SGYYDAYYKKAQKVRTLIKKDFEDVFEKYDVIVGPTTPTPAFKIGENTKDPLTMYANDIL 427 +GYYDAYY +AQKVR LI DF F + DV++GPTTP+PAF IG + DP+TMY NDI Sbjct: 365 AGYYDAYYLRAQKVRRLIADDFARAFSQVDVLMGPTTPSPAFAIGTKSDDPVTMYLNDIY 424 Query: 428 TIPVNLAGVPGISVPCGLADGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHKAKPE 484 T VNLAG+P +SVPCG DGLP+GLQI+G HF E+ + VAH +++ T H+ P+ Sbjct: 425 TSGVNLAGLPAMSVPCGFQDGLPVGLQIVGPHFAEARLLNVAHVYQRETQWHERIPK 481 Lambda K H 0.315 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 627 Number of extensions: 27 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 485 Length of database: 484 Length adjustment: 34 Effective length of query: 451 Effective length of database: 450 Effective search space: 202950 Effective search space used: 202950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
Align candidate WP_066922914.1 ACG33_RS06390 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA)
to HMM TIGR00132 (gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00132.hmm # target sequence database: /tmp/gapView.22174.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00132 [M=466] Accession: TIGR00132 Description: gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.1e-185 601.1 0.0 8e-185 600.9 0.0 1.0 1 lcl|NCBI__GCF_001579945.1:WP_066922914.1 ACG33_RS06390 Asp-tRNA(Asn)/Glu- Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_001579945.1:WP_066922914.1 ACG33_RS06390 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 600.9 0.0 8e-185 8e-185 6 465 .. 14 474 .. 9 475 .. 0.98 Alignments for each domain: == domain 1 score: 600.9 bits; conditional E-value: 8e-185 TIGR00132 6 kkkevsikevleeilerieavkdkinaflevtkekalkkakkldkkva..kekklagipiavKdniavk 72 ++++s+ e+++++l+rie+ + ++naf++vt+e+al++a++ d+++a +e l+g+p+a Kd +++ lcl|NCBI__GCF_001579945.1:WP_066922914.1 14 RARRFSSIELARHFLDRIERLNPTLNAFITVTAETALAAAEQADRRLAagEEGALIGVPLAHKDIFCTD 82 56778889999***********************************9984447**************** PP TIGR00132 73 diettcaSkiLenyvspydatVverlkeagaliiGktNlDEFamGsstetSafgvtknPeneervpGGS 141 +i ttcaS++L+n+v+pydatVv rl +aga+++Gk+N+DEFamGss etS +g+++nP++ ++vpGGS lcl|NCBI__GCF_001579945.1:WP_066922914.1 83 GILTTCASRMLSNFVAPYDATVVARLSRAGAVMLGKANMDEFAMGSSNETSWYGPVRNPWDLDKVPGGS 151 ********************************************************************* PP TIGR00132 142 sgGsaaavaadlvplalgsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiGilakkvedi 210 sgGsaaavaa lvp + g+DTGgSiRqPA++++ GlKP+YG+vSRyG++a+asSldq G l+++ +d+ lcl|NCBI__GCF_001579945.1:WP_066922914.1 152 SGGSAAAVAARLVPASTGTDTGGSIRQPAALTSLTGLKPSYGRVSRYGMIAFASSLDQAGTLTRSAHDA 220 ********************************************************************* PP TIGR00132 211 alvldvisgkDkkDstslevkveelleelkkdlkglkvgvvkelseesldkevkekfekllekleelga 279 al+l++++g D Dsts++++v++++ l++ l glk+gv+ke++e +ld+e++++++++le+ ++lga lcl|NCBI__GCF_001579945.1:WP_066922914.1 221 ALLLAQMAGFDPLDSTSVDTPVPDYVGLLAQPLTGLKIGVLKEFFERGLDEEIERRVREALEVYRRLGA 289 ********************************************************************* PP TIGR00132 280 eivevslpsvklalaiYyiispsEassnlarydgiryGkrveelkslkelyaktRsegfgeevkrRiml 348 ++vevslp++ l++++Yy+++p+E ssnl+r+dg+r+G+r + + +l +ly+++R+egfgeevkrRim lcl|NCBI__GCF_001579945.1:WP_066922914.1 290 QLVEVSLPNLPLSVPAYYVVAPAECSSNLSRFDGVRFGHRCDSPVDLLDLYKRSRAEGFGEEVKRRIMT 358 ********************************************************************* PP TIGR00132 349 GayalskeyydkyykkAqkvrtliidefeklfeevDvivsptaptlafklgekaedplemylsDvltvp 417 G+y+ls++yyd+yy++Aqkvr+li+d+f +f++vDv+++pt+p af +g k +dp++myl+D++t lcl|NCBI__GCF_001579945.1:WP_066922914.1 359 GTYVLSAGYYDAYYLRAQKVRRLIADDFARAFSQVDVLMGPTTPSPAFAIGTKSDDPVTMYLNDIYTSG 427 ********************************************************************* PP TIGR00132 418 anlaGlpaisvPlgkkekglpiGlqiigkafddkkllsvakaleqald 465 +nlaGlpa+svP+g + +glp+Glqi+g +f++ +ll+va+ ++++++ lcl|NCBI__GCF_001579945.1:WP_066922914.1 428 VNLAGLPAMSVPCGFQ-DGLPVGLQIVGPHFAEARLLNVAHVYQRETQ 474 ****************.8*************************99876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (466 nodes) Target sequences: 1 (484 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 7.73 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory