Align dihydroxyacid dehydratase (EC 4.2.1.9) (characterized)
to candidate WP_068175562.1 HTA01S_RS22650 dihydroxy-acid dehydratase
Query= metacyc::MONOMER-18815 (557 letters) >NCBI__GCF_001592305.1:WP_068175562.1 Length = 568 Score = 860 bits (2222), Expect = 0.0 Identities = 418/557 (75%), Positives = 486/557 (87%) Query: 1 MAFNKRSQNITQGVARSPNRSMYYALGYKKEDFDKPMVGIANGHSTITPCNAGLQRLADA 60 + N RS +ITQG +R+PNRSMYYA+GY++ DF KPMVG+ANGHSTITPCN+GLQ+LADA Sbjct: 10 LPINHRSAHITQGKSRAPNRSMYYAMGYEEGDFVKPMVGVANGHSTITPCNSGLQKLADA 69 Query: 61 AIDAIKASDANPQVFGTPTISDGMSMGTEGMKYSLISREVIADCIETAAQGQWMDGVVVI 120 AI I+ + N QVFGTPTISDGM+MGTEGMKYSL+SREVI+DC+ET QGQWMDGVVVI Sbjct: 70 AIAGIEEAGGNAQVFGTPTISDGMAMGTEGMKYSLVSREVISDCVETCVQGQWMDGVVVI 129 Query: 121 GGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLTIVSSFEAVGEFTAGRMSQED 180 GGCDKNMPGG++ + R NVP IYVYGGTI PG +K +DL IVS FEAVG+ AG++S D Sbjct: 130 GGCDKNMPGGLMGMLRANVPAIYVYGGTILPGRYKDQDLNIVSVFEAVGQNAAGKLSDHD 189 Query: 181 FEGVEKNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV 240 +E+ A P TGSCGGMYTANTMSS+FEALG+SL YSSTMANP EK +SA ESA+VL+ Sbjct: 190 LHEIEQRAIPGTGSCGGMYTANTMSSAFEALGISLPYSSTMANPHDEKTNSARESAKVLI 249 Query: 241 EAIKQDIKPRDIITRKSIENAVALIMATGGSTNAVLHYLAIAHAAEVEWTIDDFERIRRK 300 EAI++DIKPRDI+TRKSIENAVA+IMATGGSTNAVLH+LAIAHAA VEWTIDDFER+R+K Sbjct: 250 EAIRKDIKPRDIVTRKSIENAVAVIMATGGSTNAVLHFLAIAHAAGVEWTIDDFERVRQK 309 Query: 301 VPVICNLKPSGQYVATDLHKAGGIPQVMKILLKAGMLHGDCLTITGRTLAEELENVPDTP 360 +PV+C+LKPSG+Y+A DLH+AGGIPQVMK+LL AG+LH DCLTITG+T+AE L++VPD P Sbjct: 310 IPVLCDLKPSGKYLAVDLHRAGGIPQVMKMLLHAGLLHADCLTITGQTIAEVLKDVPDAP 369 Query: 361 RADQDVILPIEKALYAEGHLAILKGNLAEEGAVAKITGLKNPVITGPARVFEDEQSAMEA 420 RADQDVI P ++ LY GHLAILKGNL+ EG VAKITGLKNPV+TGPARVF+DEQSA++A Sbjct: 370 RADQDVIRPFDRPLYEHGHLAILKGNLSPEGCVAKITGLKNPVMTGPARVFDDEQSALQA 429 Query: 421 ILADKINAGDILVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV 480 IL I AGD++VLRYLGPKGGPGMPEMLAPT A+IG GLGESVG ITDGRFSGGTWGMV Sbjct: 430 ILDGHIVAGDVMVLRYLGPKGGPGMPEMLAPTGALIGAGLGESVGLITDGRFSGGTWGMV 489 Query: 481 VGHVAPEAYVGGTIALVQEGDSITIDAHKLLLQLNVADEELARRRANWKQPAPRYTRGVL 540 VGHVAPEA VGGTI LVQEGD ITIDAH+LLL+L+V D E+A+RRA W PAPRYTRGV Sbjct: 490 VGHVAPEAAVGGTIGLVQEGDRITIDAHRLLLELHVPDAEIAKRRAAWMAPAPRYTRGVQ 549 Query: 541 AKFSKLASTASKGAVTD 557 AKF+ AS+AS+GAV D Sbjct: 550 AKFAFNASSASQGAVLD 566 Lambda K H 0.316 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1116 Number of extensions: 39 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 557 Length of database: 568 Length adjustment: 36 Effective length of query: 521 Effective length of database: 532 Effective search space: 277172 Effective search space used: 277172 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)
Align candidate WP_068175562.1 HTA01S_RS22650 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.2128.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.2e-230 752.5 3.3 1.4e-230 752.3 3.3 1.0 1 lcl|NCBI__GCF_001592305.1:WP_068175562.1 HTA01S_RS22650 dihydroxy-acid de Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_001592305.1:WP_068175562.1 HTA01S_RS22650 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 752.3 3.3 1.4e-230 1.4e-230 1 542 [. 27 566 .. 27 567 .. 0.99 Alignments for each domain: == domain 1 score: 752.3 bits; conditional E-value: 1.4e-230 TIGR00110 1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 ++r++++a+G+++ d+ kP+++v+n++++i+P++ l++la+++ ++ie+aGg a+ f+t ++sDG+am lcl|NCBI__GCF_001592305.1:WP_068175562.1 27 PNRSMYYAMGYEEGDFVKPMVGVANGHSTITPCNSGLQKLADAAIAGIEEAGGNAQVFGTPTISDGMAM 95 68******************************************************************* PP TIGR00110 70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138 g+eGmkysL+sre+i+D vet v+++++D++vvi+ CDk++PG lm++lr n+Pai v+GG++ +g++k lcl|NCBI__GCF_001592305.1:WP_068175562.1 96 GTEGMKYSLVSREVISDCVETCVQGQWMDGVVVIGGCDKNMPGGLMGMLRANVPAIYVYGGTILPGRYK 164 ********************************************************************* PP TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207 +++++v+vfeavg+ aagkls+++l+eie+ a P++gsC+G++tan+m++++ealG+slP+sst++ lcl|NCBI__GCF_001592305.1:WP_068175562.1 165 -DQDLNIVSVFEAVGQNAAGKLSDHDLHEIEQRAIPGTGSCGGMYTANTMSSAFEALGISLPYSSTMAN 232 .8******************************************************************* PP TIGR00110 208 tsaekkelakksgkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklsld 276 + ek+++a++s+k ++e+++k+ikPrdi+t++++ena+++++a+GGstn+vLh laia+ agv+ ++d lcl|NCBI__GCF_001592305.1:WP_068175562.1 233 PHDEKTNSARESAKVLIEAIRKDIKPRDIVTRKSIENAVAVIMATGGSTNAVLHFLAIAHAAGVEWTID 301 ********************************************************************* PP TIGR00110 277 dfdrlsrkvPllaklkPsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlr. 344 df+r+++k+P+l++lkPsgk++ +dlhraGG+++v+k+l ++gllh d+lt+tG+t+ae+l++v+ + lcl|NCBI__GCF_001592305.1:WP_068175562.1 302 DFERVRQKIPVLCDLKPSGKYLAVDLHRAGGIPQVMKMLLHAGLLHADCLTITGQTIAEVLKDVPDAPr 370 ***************************************************************988753 PP TIGR00110 345 vdqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvke 413 +dqdvir+ d p++++g+la+LkGnl++eG+v+ki+g ++ +++Gpa+vf++e+ al+ail+g++ + lcl|NCBI__GCF_001592305.1:WP_068175562.1 371 ADQDVIRPFDRPLYEHGHLAILKGNLSPEGCVAKITGLKN--PVMTGPARVFDDEQSALQAILDGHIVA 437 89**************************************..889************************ PP TIGR00110 414 GdvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialv 482 Gdv+v+ry GPkGgPGm+emLaPt al+g+GLg++v+LitDGrfsGgt G+++Ghv+Peaa+gG+i+lv lcl|NCBI__GCF_001592305.1:WP_068175562.1 438 GDVMVLRYLGPKGGPGMPEMLAPTGALIGAGLGESVGLITDGRFSGGTWGMVVGHVAPEAAVGGTIGLV 506 ********************************************************************* PP TIGR00110 483 edGDkikiDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavld 542 ++GD+i+iD+++ l+l+v ++e+a+rra++ ++ +r+++g+ ak+a +ssa++Gavld lcl|NCBI__GCF_001592305.1:WP_068175562.1 507 QEGDRITIDAHRLLLELHVPDAEIAKRRAAWMAPAPRYTRGVQAKFAFNASSASQGAVLD 566 **********************************************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (568 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 11.93 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory