Align Putative acetolactate synthase large subunit IlvX; ALS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; AHAS (uncharacterized)
to candidate WP_068174522.1 HTA01S_RS20055 acetolactate synthase large subunit
Query= curated2:O53554 (515 letters) >NCBI__GCF_001592305.1:WP_068174522.1 Length = 556 Score = 548 bits (1413), Expect = e-160 Identities = 296/520 (56%), Positives = 358/520 (68%), Gaps = 11/520 (2%) Query: 2 NGAQALINTLVDGGVDVCFANPGTSEMHFVAALDAVPRMRGMLTLFEGVATGAADGYARI 61 NGAQAL+ TLVD GVDVCF NPGTSEMHFVAALD PRMR +L LFEGVATGAADGYAR+ Sbjct: 3 NGAQALMKTLVDAGVDVCFTNPGTSEMHFVAALDHEPRMRAVLALFEGVATGAADGYARM 62 Query: 62 AGRPAAVLLHLGPGLGNGLANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDAVAGT 121 AG+PAA LLHLG GLGNGLANLHNAR+ RVP+V +VGDHAT+H YDA L+SDI+ VA Sbjct: 63 AGKPAATLLHLGCGLGNGLANLHNARKGRVPVVNIVGDHATFHTVYDAQLQSDIETVARN 122 Query: 122 VS-GWVRRTEAAADVGADAEAAIAASRS-GSQIATLILPADVCWSDGAHAA--AGVPAQA 177 VS G+VR ++ A++ DA AI A+R Q+ATLILPADV W +G +P Sbjct: 123 VSPGFVRTSQTTAELCRDAADAITAARGLPGQVATLILPADVSWGEGGEPCPPPPLPQPE 182 Query: 178 AAAPVDVGPVAGVL----RSGEPAMMLIGGDATRGPGLTAAARIVQATGARWLCETFPTC 233 AA+ V +A + RSG L+GG A R PGL AAAR+ +G + L E FPT Sbjct: 183 AASDATVSAIAQAISQAARSGGKTAFLLGGQALREPGLLAAARVAAHSGVKLLAEVFPTR 242 Query: 234 LERGAGIPAVERLAYFAEGAAAQLDGVKHLVLAGARSPVSFFAYPGMPSDLVPAGCEVHV 293 LERGAG+P VER+AY AE A QL V+HLVL A++PVSFFAYPG SDLVP C VH Sbjct: 243 LERGAGLPPVERIAYLAEMAGVQLAEVQHLVLVDAKAPVSFFAYPGKKSDLVPDTCTVHT 302 Query: 294 LAEP-GGAADALAALADEVAPGTVAP-VAGASRPQLPTGDLTSVSAADVVGALLPERAIV 351 LA P A +L LA + P + A RP P G L + VG LLPE AIV Sbjct: 303 LASPEQDAVGSLHKLAAALGAAQAQPALQAAQRPGRPRGKLNAAKVCKAVGHLLPEHAIV 362 Query: 352 VDESNTCGVLLPQATAGAPAHDWLTLTGGAIGYGIPAAVGAAVAAPDRPVLCLESDGSAM 411 +DE+ T G++L TAGAP HD +TLTGGAIG G+P AVGAA+A P RPVL L DG+AM Sbjct: 363 IDEAITSGLMLSAMTAGAPRHDLITLTGGAIGQGLPNAVGAAIACPTRPVLALIGDGTAM 422 Query: 412 YTISGLWSQARENLDVTTVIYNNGAYDILRIELQRVGAGSDPGPKALDLLDISRPTMDFV 471 YT+ LW+ ARE L+VT++I+NN +Y +L +EL+RVGA ++ GPKA LD+ P + F Sbjct: 423 YTLQALWTMAREQLNVTSIIFNNASYSVLNVELERVGA-TEAGPKAKSQLDLHGPVLHFA 481 Query: 472 KIAEGMGVPARRVTTCEEFADALRAAFAEPGPHLIDVVVP 511 ++A+GMGV R TT E+F AL A A PGPHLI+ VVP Sbjct: 482 QLAQGMGVHGVRATTTEDFLKALEYALAHPGPHLIEAVVP 521 Lambda K H 0.318 0.134 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 737 Number of extensions: 30 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 515 Length of database: 556 Length adjustment: 35 Effective length of query: 480 Effective length of database: 521 Effective search space: 250080 Effective search space used: 250080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory