Alignments for a candidate for cysK in Halomonas desiderata SP1

GapMind for Amino acid biosynthesis

Alignments for a candidate for cysK in Halomonas desiderata SP1

Align Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 (characterized)
to candidate WP_086510266.1 BZY95_RS12545 cysteine synthase CysM

Query= SwissProt::P16703
         (303 letters)



>NCBI__GCF_002151265.1:WP_086510266.1
          Length = 298

 Score =  395 bits (1015), Expect = e-115
 Identities = 199/293 (67%), Positives = 235/293 (80%)

Query: 3   TLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRAALSMIVEAEKRGEIKPGD 62
           T+E  +G+TPLV+L+R+     + +  KLEGNNPAGSVKDR ALSM+ +AE RG+I PGD
Sbjct: 5   TIEDVVGHTPLVRLKRITAGRNNTLLAKLEGNNPAGSVKDRPALSMLEQAEARGDITPGD 64

Query: 63  VLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAAMRAYGAELILVTKEQGMEGA 122
            LIEATSGNTGIALAM AA+KGYRM L+MPD+ S ER+ AM AYGA LI V+KE GME A
Sbjct: 65  TLIEATSGNTGIALAMAAAIKGYRMVLIMPDSASSERKQAMAAYGARLITVSKEGGMEEA 124

Query: 123 RDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQQTGGRITHFVSSMGTTGTITGV 182
           RDLA  M +RGEGK L+QF NPDNP AHY  TGPE+W+QTGG ITHFVSSMGTTGTI GV
Sbjct: 125 RDLAEAMVSRGEGKPLNQFANPDNPLAHYRGTGPELWEQTGGTITHFVSSMGTTGTIMGV 184

Query: 183 SRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLPGIFNASLVDEVLDIHQRDAENTMR 242
           SR+++E++  V IVGLQPE+G+SI GIRRWP EYLP IF+A+ VD VLDI Q +AE  MR
Sbjct: 185 SRYLKERNPEVQIVGLQPEDGASIAGIRRWPKEYLPSIFDATRVDRVLDIGQHEAEEHMR 244

Query: 243 ELAVREGIFCGVSSGGAVAGALRVAKANPDAVVVAIICDRGDRYLSTGVFGEE 295
            LA  EGI  GVSSGGA+AGALRVA+   +AV+V I+CDRGDRYLSTG+F  E
Sbjct: 245 RLAREEGILAGVSSGGALAGALRVAEEVENAVIVFIVCDRGDRYLSTGLFAPE 297


Lambda     K      H
   0.316    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 353
Number of extensions: 11
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 303
Length of database: 298
Length adjustment: 27
Effective length of query: 276
Effective length of database: 271
Effective search space:    74796
Effective search space used:    74796
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 48 (23.1 bits)

Align candidate WP_086510266.1 BZY95_RS12545 (cysteine synthase CysM)
to HMM TIGR01138 (cysM: cysteine synthase B (EC 2.5.1.47))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01138.hmm
# target sequence database:        /tmp/gapView.22501.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01138  [M=290]
Accession:   TIGR01138
Description: cysM: cysteine synthase B
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.5e-149  482.1   0.1   3.9e-149  481.9   0.1    1.0  1  lcl|NCBI__GCF_002151265.1:WP_086510266.1  BZY95_RS12545 cysteine synthase 


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_002151265.1:WP_086510266.1  BZY95_RS12545 cysteine synthase CysM
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  481.9   0.1  3.9e-149  3.9e-149       1     290 []       5     294 ..       5     294 .. 1.00

  Alignments for each domain:
  == domain 1  score: 481.9 bits;  conditional E-value: 3.9e-149
                                 TIGR01138   1 tilklvGntplvrlkrllpeedsevlvklegnnpaGsvkdrpalsmiveaekrGeikeGdvlieatsGn 69 
                                               ti+++vG tplvrlkr+++  ++++l+klegnnpaGsvkdrpalsm+ +ae rG+i +Gd+lieatsGn
  lcl|NCBI__GCF_002151265.1:WP_086510266.1   5 TIEDVVGHTPLVRLKRITAGRNNTLLAKLEGNNPAGSVKDRPALSMLEQAEARGDITPGDTLIEATSGN 73 
                                               89******************************************************************* PP

                                 TIGR01138  70 tGialamvaalkGykvkllmpdnvseerkaalkayGaelilvdkeeGmeGardlarelvrkgeeklldq 138
                                               tGialam+aa+kGy++ l+mpd  s erk+a+ ayGa+li v+ke Gme ardla+ +v +ge+k l+q
  lcl|NCBI__GCF_002151265.1:WP_086510266.1  74 TGIALAMAAAIKGYRMVLIMPDSASSERKQAMAAYGARLITVSKEGGMEEARDLAEAMVSRGEGKPLNQ 142
                                               ********************************************************************* PP

                                 TIGR01138 139 fnnpdnpkahytstGieiwqqtkGrithfvsslGttGtimGvsrflkeqnpavqivGlqpaegsaieGl 207
                                               f+npdnp ahy  tG+e+w+qt+G ithfvss+GttGtimGvsr+lke+np+vqivGlqp++g +i G+
  lcl|NCBI__GCF_002151265.1:WP_086510266.1 143 FANPDNPLAHYRGTGPELWEQTGGTITHFVSSMGTTGTIMGVSRYLKERNPEVQIVGLQPEDGASIAGI 211
                                               ********************************************************************* PP

                                 TIGR01138 208 rrieseylpgifdaslvdrvvdveqedaediarelakkegifvGvssGgavaaalrlarelekavvvai 276
                                               rr+++eylp ifda++vdrv+d+ q++ae+ +r+la++egi+ GvssGga+a+alr+a e+e+av+v i
  lcl|NCBI__GCF_002151265.1:WP_086510266.1 212 RRWPKEYLPSIFDATRVDRVLDIGQHEAEEHMRRLAREEGILAGVSSGGALAGALRVAEEVENAVIVFI 280
                                               ********************************************************************* PP

                                 TIGR01138 277 icdrGdrylstgvf 290
                                               +cdrGdrylstg+f
  lcl|NCBI__GCF_002151265.1:WP_086510266.1 281 VCDRGDRYLSTGLF 294
                                               ************98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (290 nodes)
Target sequences:                          1  (298 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 8.88
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis