Alignments for a candidate for cysK in Halomonas desiderata SP1

GapMind for Amino acid biosynthesis

Alignments for a candidate for cysK in Halomonas desiderata SP1

Align O-ureido-L-serine synthase; Cysteine synthase homolog DscD; O-acetylserine sulfhydrylase; EC 2.6.99.3; EC 2.5.1.47 (characterized)
to candidate WP_086510419.1 BZY95_RS13365 cysteine synthase A

Query= SwissProt::D2Z027
         (324 letters)



>NCBI__GCF_002151265.1:WP_086510419.1
          Length = 490

 Score =  299 bits (765), Expect = 1e-85
 Identities = 161/332 (48%), Positives = 222/332 (66%), Gaps = 18/332 (5%)

Query: 1   MPLFNSILDTIGRTPIVRLQRMAPEHTSVYVKVESFNPGGSVKDRLALSVVLDAEAKGLL 60
           M  + SILDTIGRTP+VRL R+AP   +V+VKVE+FNP GSVKDR+AL+V+  AE  G L
Sbjct: 1   MTRYASILDTIGRTPLVRLARLAPPTVNVHVKVEAFNPMGSVKDRMALAVIEAAERSGDL 60

Query: 61  KPGDTIVECTSGNVGIALAMVAAARGYRFVAVMGDTYSVERRKLIRAYGGKLVLFPGHLG 120
           +PG T++E TSGN GI LAMV A +GY  V  M +++S+ERR+L+R  G ++VL P    
Sbjct: 61  RPGQTVIEATSGNTGIGLAMVCARKGYPLVVTMAESFSLERRRLLRFLGARVVLTPA--A 118

Query: 121 SKGGNLIAD--ELAEKYGWFRARQFDNPANPSYHRETTASEILADFAGKRLDHFVTGFGT 178
            KG  ++A   ELAE++G+F  RQF+N AN   H  TTA EIL D  G+ +  +V+GFGT
Sbjct: 119 EKGSGMLAKAIELAERHGYFLCRQFENEANAEVHSRTTAREILDDMQGEPIHAWVSGFGT 178

Query: 179 TGTLTGVGQMLRVARPEVRVVALEPSNAAMLARGE----------WSPHQIQGLAPNFVP 228
            GTL GV ++L+ A P +R+V  EP NA +L  GE          + PH +QG +P+F+ 
Sbjct: 179 GGTLKGVSRVLKAADPRIRIVVAEPDNAPLLGSGEVQPAGVSHPRFRPHLMQGWSPDFIS 238

Query: 229 GVLDRSV----IDDLVTMDEVTARDTSRRLAAEEGIFAGISAGATVATALSIAEHAPEGT 284
            +  ++V    +DD+V +    A   +R LA +EGIF GISAGAT+A AL +A  AP G+
Sbjct: 239 PLTQQAVAADMVDDVVPVAGDEALRLARELARKEGIFVGISAGATLAAALEVARRAPAGS 298

Query: 285 VLLAMLPDTGERYLSTFLFDGVDEGSDDAWLA 316
            ++ MLPDTGERY ST LF+G+++  ++  LA
Sbjct: 299 HIVCMLPDTGERYQSTPLFEGIEDEMNEEELA 330


Lambda     K      H
   0.319    0.136    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 425
Number of extensions: 23
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 324
Length of database: 490
Length adjustment: 31
Effective length of query: 293
Effective length of database: 459
Effective search space:   134487
Effective search space used:   134487
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_086510419.1 BZY95_RS13365 (cysteine synthase A)
to HMM TIGR01139 (cysK: cysteine synthase A (EC 2.5.1.47))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01139.hmm
# target sequence database:        /tmp/gapView.31334.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01139  [M=298]
Accession:   TIGR01139
Description: cysK: cysteine synthase A
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
     3e-112  360.7   0.0   3.7e-112  360.4   0.0    1.1  1  lcl|NCBI__GCF_002151265.1:WP_086510419.1  BZY95_RS13365 cysteine synthase 


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_002151265.1:WP_086510419.1  BZY95_RS13365 cysteine synthase A
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  360.4   0.0  3.7e-112  3.7e-112       2     298 .]       8     317 ..       7     317 .. 0.96

  Alignments for each domain:
  == domain 1  score: 360.4 bits;  conditional E-value: 3.7e-112
                                 TIGR01139   2 seliGntPlvrLnlaeeakaevlvkleslnPsssvkdrialamiedaekegllkkgktiveatsGntGi 70 
                                                ++iG+tPlvrL + ++ +++v vk+e +nP +svkdr+ala+ie ae++g l++g+t++eatsGntGi
  lcl|NCBI__GCF_002151265.1:WP_086510419.1   8 LDTIGRTPLVRLARLAPPTVNVHVKVEAFNPMGSVKDRMALAVIEAAERSGDLRPGQTVIEATSGNTGI 76 
                                               579**********99999*************************************************** PP

                                 TIGR01139  71 alamvaaargykliltmpetmslerrkllkayGaelvLtdgaegmkgaiekaeelveetpnkylllkqf 139
                                               +lamv+a +gy l++tm+e++slerr+ll+ +Ga++vLt++ae+ +g+++ka el+e+++  y+l +qf
  lcl|NCBI__GCF_002151265.1:WP_086510419.1  77 GLAMVCARKGYPLVVTMAESFSLERRRLLRFLGARVVLTPAAEKGSGMLAKAIELAERHG--YFLCRQF 143
                                               ************************************************************..99***** PP

                                 TIGR01139 140 enpanpeihrkttapeilkdldgk.ldafvagvGtGGtitGvgevlkekkpdikvvavePaespvlsgg 207
                                               en+an e+h +tta+eil d++g+ + a v+g+GtGGt+ Gv++vlk ++p i++v++eP ++p+l  g
  lcl|NCBI__GCF_002151265.1:WP_086510419.1 144 ENEANAEVHSRTTAREILDDMQGEpIHAWVSGFGTGGTLKGVSRVLKAADPRIRIVVAEPDNAPLLGSG 212
                                               ***********************99*****************************************998 PP

                                 TIGR01139 208 ..........kpgphkiqGigagfiP....kvLdkevidevikvsdeeaietarrlakeeGilvGissG 262
                                                         +  ph +qG++++fi     + +  +++d+v+ v  +ea+++ar+la++eGi+vGis+G
  lcl|NCBI__GCF_002151265.1:WP_086510419.1 213 evqpagvshpRFRPHLMQGWSPDFISpltqQAVAADMVDDVVPVAGDEALRLARELARKEGIFVGISAG 281
                                               8999999998899************7222244567889******************************* PP

                                 TIGR01139 263 aavaaalkvakklekdkkivvilpdtgerYlstaLf 298
                                               a++aaal+va+++ ++ +iv++lpdtgerY st+Lf
  lcl|NCBI__GCF_002151265.1:WP_086510419.1 282 ATLAAALEVARRAPAGSHIVCMLPDTGERYQSTPLF 317
                                               ***********************************9 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (298 nodes)
Target sequences:                          1  (490 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 10.10
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis