Alignments for a candidate for leuD in Halomonas desiderata SP1

GapMind for Amino acid biosynthesis

Alignments for a candidate for leuD in Halomonas desiderata SP1

Align 3-isopropylmalate dehydratase small subunit; EC 4.2.1.33 (characterized, see rationale)
to candidate WP_086508766.1 BZY95_RS04405 3-isopropylmalate dehydratase small subunit

Query= uniprot:Q845W4
         (216 letters)



>NCBI__GCF_002151265.1:WP_086508766.1
          Length = 217

 Score =  294 bits (753), Expect = 8e-85
 Identities = 143/210 (68%), Positives = 162/210 (77%)

Query: 1   MEKFTVHTGVVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSK 60
           M KF    G+VAPLDR NVDTD IIPKQFLKSIKRTGFG N FDE RYLD G+PGQD S+
Sbjct: 1   MRKFERTEGLVAPLDRANVDTDLIIPKQFLKSIKRTGFGVNLFDELRYLDEGQPGQDCSQ 60

Query: 61  RPLNPDFVLNQPRYQGASILVTRKNFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNC 120
           RPLNPDFVLNQPRYQGAS+L+ R+NFGCGSSREHAPWAL+ +GFR +IAPSFADIF+NN 
Sbjct: 61  RPLNPDFVLNQPRYQGASVLLARRNFGCGSSREHAPWALEDFGFRVVIAPSFADIFYNNA 120

Query: 121 FKNGLLPIVLTEQQVDHLINETVAFNGYQLTIDLEAQVVRTPDGRDYPFEITAFRKYCLL 180
           FKNGLL I L E+ VD L  E  A  GY+L +DLE Q V TP G    FE+ AFRK+CLL
Sbjct: 121 FKNGLLLITLPEEVVDRLFREVEASEGYRLDVDLENQRVITPAGEILEFEVDAFRKHCLL 180

Query: 181 NGFDDIGLTLRHADKIRQFEAERLAKQPWL 210
            G DDIG+TL+  D IR FE +    +PWL
Sbjct: 181 EGLDDIGITLQDEDAIRAFEVKHRQARPWL 210


Lambda     K      H
   0.322    0.141    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 197
Number of extensions: 3
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 216
Length of database: 217
Length adjustment: 22
Effective length of query: 194
Effective length of database: 195
Effective search space:    37830
Effective search space used:    37830
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 45 (21.9 bits)

Align candidate WP_086508766.1 BZY95_RS04405 (3-isopropylmalate dehydratase small subunit)
to HMM TIGR00171 (leuD: 3-isopropylmalate dehydratase, small subunit (EC 4.2.1.33))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00171.hmm
# target sequence database:        /tmp/gapView.3114.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00171  [M=188]
Accession:   TIGR00171
Description: leuD: 3-isopropylmalate dehydratase, small subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
      8e-87  276.0   0.0    9.4e-87  275.8   0.0    1.1  1  lcl|NCBI__GCF_002151265.1:WP_086508766.1  BZY95_RS04405 3-isopropylmalate 


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_002151265.1:WP_086508766.1  BZY95_RS04405 3-isopropylmalate dehydratase small subunit
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  275.8   0.0   9.4e-87   9.4e-87       1     188 []       1     196 [.       1     196 [. 0.95

  Alignments for each domain:
  == domain 1  score: 275.8 bits;  conditional E-value: 9.4e-87
                                 TIGR00171   1 mkefkkltGlvvpldkanvdtdaiipkqflkkikrtGfgkhlfyewrylde..kGkep.....npefvl 62 
                                               m++f + +Glv+pld+anvdtd iipkqflk ikrtGfg +lf e rylde   G++      np+fvl
  lcl|NCBI__GCF_002151265.1:WP_086508766.1   1 MRKFERTEGLVAPLDRANVDTDLIIPKQFLKSIKRTGFGVNLFDELRYLDEgqPGQDCsqrplNPDFVL 69 
                                               8899*********************************************963235542333459***** PP

                                 TIGR00171  63 nvpqyqgasillarenfGcGssrehapwalkdyGfkviiapsfadifynnsfkngllpirlseeeveel 131
                                               n+p+yqgas+llar nfGcGssrehapwal+d+Gf+v+iapsfadifynn+fkngll i l+ee v+ l
  lcl|NCBI__GCF_002151265.1:WP_086508766.1  70 NQPRYQGASVLLARRNFGCGSSREHAPWALEDFGFRVVIAPSFADIFYNNAFKNGLLLITLPEEVVDRL 138
                                               ********************************************************************* PP

                                 TIGR00171 132 lalvk.nkglkltvdleaqkvkdsegkvysfeidefrkhcllnGldeigltlqkedei 188
                                               +  v+  +g +l+vdle+q+v +  g++ +fe+d+frkhcll+Gld+ig+tlq ed+i
  lcl|NCBI__GCF_002151265.1:WP_086508766.1 139 FREVEaSEGYRLDVDLENQRVITPAGEILEFEVDAFRKHCLLEGLDDIGITLQDEDAI 196
                                               ****999*************************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (188 nodes)
Target sequences:                          1  (217 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 9.14
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis