Alignments for a candidate for dapD in Halomonas desiderata SP1

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapD in Halomonas desiderata SP1

Align 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase; Tetrahydrodipicolinate N-succinyltransferase; THDP succinyltransferase; THP succinyltransferase; Tetrahydropicolinate succinylase; EC 2.3.1.117 (characterized)
to candidate WP_086509350.1 BZY95_RS07625 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase

Query= SwissProt::G3XD76
         (344 letters)



>NCBI__GCF_002151265.1:WP_086509350.1
          Length = 341

 Score =  464 bits (1193), Expect = e-135
 Identities = 234/341 (68%), Positives = 269/341 (78%), Gaps = 1/341 (0%)

Query: 5   LFSLAFGVGTQNRQEAWLEVFYALPLLKPSSEIVAAVAPILGYAAGNQALTFTSQQAYQL 64
           + S AFG+GTQN Q  WLE++Y  PL KP + +V AV  +L   +GN A++F  +    +
Sbjct: 1   MLSFAFGIGTQNTQGDWLEIYYPAPLFKPDASLVEAVGKVLDIPSGNAAVSFLPEHCSDI 60

Query: 65  ADALKGID-AAQSALLSRLAESQKPLVATLLAEDAAPSSTAEAYLKLHLLSHRLVKPHAV 123
           A+ALK     AQ+ L   LA SQ+PLVAT +  D  P S  E YLKLHLLSHRLVKPH +
Sbjct: 61  AEALKAAGHVAQAELAEALAGSQRPLVATFIDSDVPPQSVPEVYLKLHLLSHRLVKPHGL 120

Query: 124 NLSGIFPLLPNVAWTNIGAVDLAELAELQLEARLKGKLLEVFSVDKFPKMTDYVVPAGVR 183
           +L+G+F LL NVAWT+ G +D+ EL   +L+ARL+G+ L V  VDKFPKMTDYVVP+GVR
Sbjct: 121 DLTGMFGLLRNVAWTSEGPIDIEELPARRLKARLEGRALSVDCVDKFPKMTDYVVPSGVR 180

Query: 184 IADTARVRLGAYIGEGTTVMHEGFVNFNAGTEGPGMIEGRVSAGVFVGKGSDLGGGCSTM 243
           IADTARVRLGAY+GEGTTVMHEGFVNFNAG EGPGMIEGR+SAGV VGKGSDLGGGCSTM
Sbjct: 181 IADTARVRLGAYLGEGTTVMHEGFVNFNAGAEGPGMIEGRISAGVLVGKGSDLGGGCSTM 240

Query: 244 GTLSGGGNIVISVGEGCLIGANAGIGIPLGDRNIVEAGLYITAGTKVALLDEQNALVKVV 303
           GTLSGGGNIVI VGEGCLIGANAGIGIPLGDR  VEAGLYITAG KV LLD+Q   VK V
Sbjct: 241 GTLSGGGNIVIKVGEGCLIGANAGIGIPLGDRCTVEAGLYITAGAKVTLLDDQGQEVKTV 300

Query: 304 KARDLAGQPDLLFRRNSQNGAVECKTNKTAIELNEALHAHN 344
            AR+LAGQ DLL RRNSQNG +EC TNK+AI LNEALHA+N
Sbjct: 301 AARELAGQSDLLLRRNSQNGRIECLTNKSAIALNEALHANN 341


Lambda     K      H
   0.317    0.134    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 400
Number of extensions: 13
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 344
Length of database: 341
Length adjustment: 29
Effective length of query: 315
Effective length of database: 312
Effective search space:    98280
Effective search space used:    98280
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate WP_086509350.1 BZY95_RS07625 (2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase)
to HMM TIGR03536 (dapD: 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR03536.hmm
# target sequence database:        /tmp/gapView.6389.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03536  [M=341]
Accession:   TIGR03536
Description: DapD_gpp: 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.2e-190  617.7   0.2   2.5e-190  617.5   0.2    1.0  1  lcl|NCBI__GCF_002151265.1:WP_086509350.1  BZY95_RS07625 2,3,4,5-tetrahydro


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_002151265.1:WP_086509350.1  BZY95_RS07625 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransf
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  617.5   0.2  2.5e-190  2.5e-190       1     341 []       1     341 []       1     341 [] 1.00

  Alignments for each domain:
  == domain 1  score: 617.5 bits;  conditional E-value: 2.5e-190
                                 TIGR03536   1 llslalGvgtknrkgewlevffptpllnpdeelveavaealgyegGnqaieltaeklealaaalkeagl 69 
                                               +ls+a+G+gt+n++g+wle+++p+pl+ pd++lveav ++l+  +Gn+a+++ +e+++++a+alk+ag+
  lcl|NCBI__GCF_002151265.1:WP_086509350.1   1 MLSFAFGIGTQNTQGDWLEIYYPAPLFKPDASLVEAVGKVLDIPSGNAAVSFLPEHCSDIAEALKAAGH 69 
                                               689****************************************************************** PP

                                 TIGR03536  70 eeqaelaaklkeskrplvavlleedaapasvpeaylklqllshrlvkphgvnleGifgllknvawtneG 138
                                                +qaela++l+ s+rplva+++++d  p svpe+ylkl+llshrlvkphg++l+G+fgll+nvawt+eG
  lcl|NCBI__GCF_002151265.1:WP_086509350.1  70 VAQAELAEALAGSQRPLVATFIDSDVPPQSVPEVYLKLHLLSHRLVKPHGLDLTGMFGLLRNVAWTSEG 138
                                               ********************************************************************* PP

                                 TIGR03536 139 aidleelaerqlearlnGevlevdsvdkfpkmtdyvvpkGvriadtarvrlGayvGeGttvmheGfvnf 207
                                               +id+eel++r+l+arl+G++l+vd+vdkfpkmtdyvvp+GvriadtarvrlGay+GeGttvmheGfvnf
  lcl|NCBI__GCF_002151265.1:WP_086509350.1 139 PIDIEELPARRLKARLEGRALSVDCVDKFPKMTDYVVPSGVRIADTARVRLGAYLGEGTTVMHEGFVNF 207
                                               ********************************************************************* PP

                                 TIGR03536 208 naGteGasmveGrisaGvlvGkGsdlGGGastmGtlsGGGnivisvGeecllGanaGiGiplGdrctve 276
                                               naG+eG++m+eGrisaGvlvGkGsdlGGG+stmGtlsGGGnivi+vGe+cl+GanaGiGiplGdrctve
  lcl|NCBI__GCF_002151265.1:WP_086509350.1 208 NAGAEGPGMIEGRISAGVLVGKGSDLGGGCSTMGTLSGGGNIVIKVGEGCLIGANAGIGIPLGDRCTVE 276
                                               ********************************************************************* PP

                                 TIGR03536 277 aGlyitaGtkvallddkgelvevvkardlaGksdllfrrnslnGavecltnksaielneelhann 341
                                               aGlyitaG+kv+lldd+g+ v++v ar+laG+sdll+rrns+nG++ecltnksai+lne+lhann
  lcl|NCBI__GCF_002151265.1:WP_086509350.1 277 AGLYITAGAKVTLLDDQGQEVKTVAARELAGQSDLLLRRNSQNGRIECLTNKSAIALNEALHANN 341
                                               ***************************************************************98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (341 nodes)
Target sequences:                          1  (341 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 11.27
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis