Align diaminopimelate decarboxylase (EC 4.1.1.20) (characterized)
to candidate WP_086511731.1 BZY95_RS20455 diaminopimelate decarboxylase
Query= BRENDA::Q9KVL7 (417 letters) >NCBI__GCF_002151265.1:WP_086511731.1 Length = 430 Score = 563 bits (1452), Expect = e-165 Identities = 285/420 (67%), Positives = 340/420 (80%), Gaps = 5/420 (1%) Query: 1 MDYFNYQEDGQLWAEQVPLADLANQYGTPLYVYSRATLERHWHAFDKSVGDYPHLICYAV 60 MD+F Y+ DG L+ E+VPL +A++ GTP YVYSRATL RH+ A+ +++G +PHLICYAV Sbjct: 1 MDHFEYR-DGVLYGEEVPLTQVADEIGTPCYVYSRATLTRHYRAYTEALGSHPHLICYAV 59 Query: 61 KANSNLGVLNTLARLGSGFDIVSVGELERVLAAGGDPSKVVFSGVGKTEAEMKRALQLKI 120 KANSNL VLN LARLG+GFDIVS+GELERVLAAGGDP+KVVFSGV K E EM RAL++ I Sbjct: 60 KANSNLAVLNLLARLGAGFDIVSLGELERVLAAGGDPAKVVFSGVAKQEHEMARALEVGI 119 Query: 121 KCFNVESEPELQRLNKVAGELGVKAPISLRINPDVDAKTHPYISTGLRDNKFGITFDRAA 180 KCFNVES PEL+RL+ VA LG AP+SLR+NPDVDA THPYISTGL+DNKFGI A Sbjct: 120 KCFNVESRPELERLSAVAERLGKVAPVSLRVNPDVDAGTHPYISTGLKDNKFGIAVGEAL 179 Query: 181 QVYRLAHSLPNLDVHGIDCHIGSQLTALAPFIDATDRLLALIDSLKAEGIHIRHLDVGGG 240 +VY LA LP L V GIDCHIGSQLT LAPF+DA DRLL L++ L GI + HLD+GGG Sbjct: 180 EVYELAARLPGLKVVGIDCHIGSQLTELAPFLDALDRLLVLLERLHERGISVEHLDLGGG 239 Query: 241 LGVVYRDELPPQPSEYAKALLDRLERHR---DLELIFEPGRAIAANAGVLVTKVEFLKHT 297 LGV YR E PPQP +YA +LL+RL + + +L L+FEPGR+IAANAGVL+T+VEFLK Sbjct: 240 LGVPYRGERPPQPFDYAASLLERLAQWKHGENLTLLFEPGRSIAANAGVLLTRVEFLKPG 299 Query: 298 EHKNFAIIDAAMNDLIRPALYQAWQDIIPLRPRQ-GEAQTYDLVGPVCETSDFLGKDRDL 356 E KNFAI+DA MNDLIRP+LYQAWQ IIP+ R E+ YD+VGPVCET DFLGK+R+L Sbjct: 300 ETKNFAIVDAGMNDLIRPSLYQAWQAIIPVDTRHVRESAVYDVVGPVCETGDFLGKEREL 359 Query: 357 VLQEGDLLAVRSSGAYGFTMSSNYNTRPRVAEVMVDGNKTYLVRQREELSSLWALESVLP 416 + GDLLAVRS+GAYGF M+SNYN+RPR AEVMVDG+ ++VRQRE + SLWA ES+LP Sbjct: 360 AIGAGDLLAVRSAGAYGFVMASNYNSRPRPAEVMVDGDAYHVVRQRESVESLWAGESLLP 419 Lambda K H 0.319 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 572 Number of extensions: 26 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 417 Length of database: 430 Length adjustment: 32 Effective length of query: 385 Effective length of database: 398 Effective search space: 153230 Effective search space used: 153230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate WP_086511731.1 BZY95_RS20455 (diaminopimelate decarboxylase)
to HMM TIGR01048 (lysA: diaminopimelate decarboxylase (EC 4.1.1.20))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01048.hmm # target sequence database: /tmp/gapView.8378.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01048 [M=417] Accession: TIGR01048 Description: lysA: diaminopimelate decarboxylase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.1e-165 536.2 0.0 2.6e-165 535.9 0.0 1.0 1 lcl|NCBI__GCF_002151265.1:WP_086511731.1 BZY95_RS20455 diaminopimelate de Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_002151265.1:WP_086511731.1 BZY95_RS20455 diaminopimelate decarboxylase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 535.9 0.0 2.6e-165 2.6e-165 5 416 .. 7 415 .. 4 416 .. 0.98 Alignments for each domain: == domain 1 score: 535.9 bits; conditional E-value: 2.6e-165 TIGR01048 5 kdgeleiegvdlkelaeefgtPlYvydeetlrerlealkeafkaeeslvlYAvKAnsnlavlrllaeeG 73 +dg l+ e+v+l+++a+e gtP Yvy+++tl+++++a++ea +++ +l++YAvKAnsnlavl+lla++G lcl|NCBI__GCF_002151265.1:WP_086511731.1 7 RDGVLYGEEVPLTQVADEIGTPCYVYSRATLTRHYRAYTEALGSHPHLICYAVKANSNLAVLNLLARLG 75 6889999************************************************************** PP TIGR01048 74 lgldvvsgGEleralaAgvkaekivfsgngkseeeleaaleleiklinvdsveelelleeiakelgkka 142 +g+d+vs GEler+laAg ++ k+vfsg++k+e+e+++ale++ik++nv+s ele+l+++a++lgk a lcl|NCBI__GCF_002151265.1:WP_086511731.1 76 AGFDIVSLGELERVLAAGGDPAKVVFSGVAKQEHEMARALEVGIKCFNVESRPELERLSAVAERLGKVA 144 ********************************************************************* PP TIGR01048 143 rvllRvnpdvdaktheyisTGlkesKFGieveeaeeayelalkleslelvGihvHIGSqildlepfvea 211 +v+lRvnpdvda th+yisTGlk++KFGi+v ea+e+yela++l+ l++vGi++HIGSq+++l+pf +a lcl|NCBI__GCF_002151265.1:WP_086511731.1 145 PVSLRVNPDVDAGTHPYISTGLKDNKFGIAVGEALEVYELAARLPGLKVVGIDCHIGSQLTELAPFLDA 213 ********************************************************************* PP TIGR01048 212 aekvvklleelkeegieleeldlGGGlgisyeeeeeapdleeyaeklleklekeaelglklklilEpGR 280 +++++ lle+l+e+gi++e+ldlGGGlg++y+ e +p++ +ya+ lle+l++ ++g++l+l++EpGR lcl|NCBI__GCF_002151265.1:WP_086511731.1 214 LDRLLVLLERLHERGISVEHLDLGGGLGVPYRGER-PPQPFDYAASLLERLAQ-WKHGENLTLLFEPGR 280 *********************************99.****************9.59999********** PP TIGR01048 281 slvanagvlltrVesvKevesrkfvlvDagmndliRpalYeayheiaalkrleeeetetvdvvGplCEs 349 s++anagvlltrVe++K e+++f++vDagmndliRp+lY+a++ i+++++ + +e++++dvvGp+CE+ lcl|NCBI__GCF_002151265.1:WP_086511731.1 281 SIAANAGVLLTRVEFLKPGETKNFAIVDAGMNDLIRPSLYQAWQAIIPVDTRHVRESAVYDVVGPVCET 349 ***************************************************999*************** PP TIGR01048 350 gDvlakdrelpeveeGdllavasaGAYgasmssnYnsrprpaevlveegkarlirrretledllale 416 gD+l+k+rel Gdllav+saGAYg+ m+snYnsrprpaev+v+++ ++++r+re++e+l+a e lcl|NCBI__GCF_002151265.1:WP_086511731.1 350 GDFLGKERELAIGA-GDLLAVRSAGAYGFVMASNYNSRPRPAEVMVDGDAYHVVRQRESVESLWAGE 415 **********8766.*************************************************976 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (417 nodes) Target sequences: 1 (430 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 7.50 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory