Alignments for a candidate for trpB in Halomonas desiderata SP1

GapMind for Amino acid biosynthesis

Alignments for a candidate for trpB in Halomonas desiderata SP1

Align Tryptophan synthase beta chain 1; EC 4.2.1.20 (characterized, see rationale)
to candidate WP_086508780.1 BZY95_RS04475 tryptophan synthase subunit beta

Query= uniprot:P50383
         (425 letters)



>NCBI__GCF_002151265.1:WP_086508780.1
          Length = 404

 Score =  130 bits (328), Expect = 6e-35
 Identities = 114/364 (31%), Positives = 165/364 (45%), Gaps = 55/364 (15%)

Query: 72  IGRPTPLFRAKRLEEYLKTPARIYFKYEGATPTGSHKINTAIPQAYFAKEEGIEHVVTET 131
           +GRP+PL+ A+R  + L   ARI+ K E    TG+HK+N  I QA  AK  G   V+ ET
Sbjct: 61  VGRPSPLYHAERWSKKLGG-ARIWLKREDLNHTGAHKVNNTIGQALLAKMSGKPRVIAET 119

Query: 132 GAGQWGTAVALAASMYNMKSTIFMVKVSYEQKPMRRSIMQLYGANVYASPTNLTEYGRKI 191
           GAGQ G A A  A+   +K  ++M     +++ +    M+L GANV        E G + 
Sbjct: 120 GAGQHGVATATVAARLGLKCEVYMGAEDVQRQKLNVYRMRLLGANVIP-----VESGTRT 174

Query: 192 LETNPQHPGSLGIAMSEAIEYALKN--EFRYLVGSVLD------VVLLHQSVIGQETITQ 243
           L+           AM+EA+   + N  +  Y++G+V        +V    +V+G+E   Q
Sbjct: 175 LKD----------AMNEALRDWVTNVDDTFYIIGTVAGPHPYPMLVRDFNAVVGREARRQ 224

Query: 244 -LDLLGEDADILIGCVGGGSNFGGFTYPFI---------------GNKKGKRYIAVSSAE 287
            L+  G+  D LI CVGGGSN  G  YPF+               G + G+    ++S  
Sbjct: 225 SLEEFGKLPDALIACVGGGSNAMGLFYPFVEDDEVAMYGVEAGGDGVETGRHAAPLASNA 284

Query: 288 IPKFSKGEYKYDFPDSAGLLPLVKMITLGKDYVPPPIYAGGLRYHGVAPTLSLLTKEGIV 347
                 G   Y   D  G +     I+ G D            Y GV P  +L    G V
Sbjct: 285 PRGVLHGNRTYLMSDEGGQVSDTHSISAGLD------------YPGVGPEHALWKDVGRV 332

Query: 348 EWREYNEREIFEAAKIFIENQGIVPAPESAHAIRAVVDEAIEARKNNERKVIVFNLSGHG 407
           ++   N+  + EA +     +GI+PA ESAHA+      A   R +     IV NLSG G
Sbjct: 333 QYVAANDDAVLEAFRELTRIEGIMPALESAHALAHAKVLAPTMRPDQN---IVVNLSGRG 389

Query: 408 LLDL 411
             D+
Sbjct: 390 DKDI 393


Lambda     K      H
   0.318    0.138    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 358
Number of extensions: 22
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 425
Length of database: 404
Length adjustment: 31
Effective length of query: 394
Effective length of database: 373
Effective search space:   146962
Effective search space used:   146962
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_086508780.1 BZY95_RS04475 (tryptophan synthase subunit beta)
to HMM TIGR00263 (trpB: tryptophan synthase, beta subunit (EC 4.2.1.20))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00263.hmm
# target sequence database:        /tmp/gapView.24116.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00263  [M=385]
Accession:   TIGR00263
Description: trpB: tryptophan synthase, beta subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   4.8e-186  604.4   0.0   5.4e-186  604.2   0.0    1.0  1  lcl|NCBI__GCF_002151265.1:WP_086508780.1  BZY95_RS04475 tryptophan synthas


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_002151265.1:WP_086508780.1  BZY95_RS04475 tryptophan synthase subunit beta
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  604.2   0.0  5.4e-186  5.4e-186       1     383 [.      15     398 ..      15     400 .. 0.99

  Alignments for each domain:
  == domain 1  score: 604.2 bits;  conditional E-value: 5.4e-186
                                 TIGR00263   1 gkfgefGGqyvpevllealeelekayekakkdeefkkeleellkeyagrptpltfaknlskklggakiy 69 
                                               g+fg +GG++v+e+l  al++lek+y ++++d++f++e+++ l +y+grp+pl++a++ skklgga+i+
  lcl|NCBI__GCF_002151265.1:WP_086508780.1  15 GHFGPYGGRFVSETLSFALDDLEKTYMRLRDDPDFQAEFDYDLAHYVGRPSPLYHAERWSKKLGGARIW 83 
                                               68******************************************************************* PP

                                 TIGR00263  70 lkredllhtGahkinnalgqallakrlGkkriiaetGaGqhGvatataaallglecevymGaedverqk 138
                                               lkredl+htGahk+nn++gqallak  Gk r+iaetGaGqhGvatat+aa+lgl+cevymGaedv+rqk
  lcl|NCBI__GCF_002151265.1:WP_086508780.1  84 LKREDLNHTGAHKVNNTIGQALLAKMSGKPRVIAETGAGQHGVATATVAARLGLKCEVYMGAEDVQRQK 152
                                               ********************************************************************* PP

                                 TIGR00263 139 lnvfrmellgakvvpvtsGsktlkdavnealrdWvtsvedthyvlGsavGphPfPeivrefqsvigeev 207
                                               lnv+rm+llga+v+pv+sG++tlkda+nealrdWvt+v+dt+y++G+++GphP+P +vr+f +v+g+e+
  lcl|NCBI__GCF_002151265.1:WP_086508780.1 153 LNVYRMRLLGANVIPVESGTRTLKDAMNEALRDWVTNVDDTFYIIGTVAGPHPYPMLVRDFNAVVGREA 221
                                               ********************************************************************* PP

                                 TIGR00263 208 keqilekegrlPdaviacvGGGsnaiGifaafiedeeveligveagGkGidtekhaatlskGk.eGvlh 275
                                               ++q le+ g+lPda+iacvGGGsna+G+f++f+ed+ev+++gveagG G++t +haa l++    Gvlh
  lcl|NCBI__GCF_002151265.1:WP_086508780.1 222 RRQSLEEFGKLPDALIACVGGGSNAMGLFYPFVEDDEVAMYGVEAGGDGVETGRHAAPLASNApRGVLH 290
                                               ******99***************************************************987658**** PP

                                 TIGR00263 276 GaktkllqdedGqieeahsvsaGldypgvgPehaalaetgraeyeaitdeealealkllskeeGiipal 344
                                               G++t+l+ de Gq++++hs+saGldypgvgPeha  ++ gr++y+a++d+++lea++ l++ eGi+pal
  lcl|NCBI__GCF_002151265.1:WP_086508780.1 291 GNRTYLMSDEGGQVSDTHSISAGLDYPGVGPEHALWKDVGRVQYVAANDDAVLEAFRELTRIEGIMPAL 359
                                               ********************************************************************* PP

                                 TIGR00263 345 esshalaaleklapklkkdeivvvnlsGrGdkdletvak 383
                                               es+hala+++ lap+++ d+ +vvnlsGrGdkd+ tvak
  lcl|NCBI__GCF_002151265.1:WP_086508780.1 360 ESAHALAHAKVLAPTMRPDQNIVVNLSGRGDKDIMTVAK 398
                                               *************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (385 nodes)
Target sequences:                          1  (404 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 10.70
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis