Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate WP_092991507.1 BLP65_RS00420 3-deoxy-7-phosphoheptulonate synthase
Query= BRENDA::Q9WYH8 (338 letters) >NCBI__GCF_900102855.1:WP_092991507.1 Length = 338 Score = 332 bits (851), Expect = 8e-96 Identities = 168/335 (50%), Positives = 230/335 (68%), Gaps = 1/335 (0%) Query: 1 MIVVLKPGSTEEDIRKVVKLAESYNLKCHISKGQERTVIGIIGDDRYVVADKFESLDCVE 60 MI+++ G +EE I V K ES L ++S+G ERTVIG IGD+ + ++L VE Sbjct: 1 MIIIMSTGVSEEHIAGVEKRLESMGLGSNVSRGIERTVIGAIGDETKADKEALQALPGVE 60 Query: 61 SVVRVLKPYKLVSREFHPEDTVIDLGDVKIGNGYFTIIAGPCSVEGREMLMETAHFLSEL 120 V+RV+KPYK+V+R +H EDTV+D+G VK+G +IAGPCSVE +E + A + Sbjct: 61 DVIRVMKPYKIVARSYHKEDTVVDIGGVKLGGNTVQVIAGPCSVETQEQMDRAAEMVVAA 120 Query: 121 GVKVLRGGAYKPRTSPYSFQGLGEKGLEYLREAADKYGMYVVTEALGEDDLPKVAEY-AD 179 G +++RGGA+KPRTSPYSFQG G +GLE R AADK + +VTE + L EY D Sbjct: 121 GCRLMRGGAFKPRTSPYSFQGHGVEGLEIFRAAADKQKLPIVTELMDVRSLDAFDEYNVD 180 Query: 180 IIQIGARNAQNFRLLSKAGSYNKPVLLKRGFMNTIEEFLLSAEYIANSGNTKIILCERGI 239 IQIG RN QNF LL + G KPV+LKRG TI+E+L+SAEYIA GN II CERGI Sbjct: 181 AIQIGTRNMQNFDLLKEVGRIQKPVILKRGMSATIKEWLMSAEYIAAGGNHNIIFCERGI 240 Query: 240 RTFEKATRNTLDISAVPIIRKESHLPILVDPSHSGGRRDLVIPLSRAAIAVGAHGIIVEV 299 RTFE RN LD++A+P+++KE+HLP++VDPSH+GG+ +V L+RAA+A GA G++VE+ Sbjct: 241 RTFETYYRNVLDVTAIPVLKKETHLPVIVDPSHAGGKAWMVPALARAAVAAGADGLLVEM 300 Query: 300 HPEPEKALSDGKQSLDFELFKELVQEMKKLADALG 334 HP P +A D Q++ + L+ E++ +A A+G Sbjct: 301 HPSPCEAWCDADQAITPDELNALMGELRGIAKAIG 335 Lambda K H 0.318 0.138 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 316 Number of extensions: 7 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 338 Length of database: 338 Length adjustment: 28 Effective length of query: 310 Effective length of database: 310 Effective search space: 96100 Effective search space used: 96100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
Align candidate WP_092991507.1 BLP65_RS00420 (3-deoxy-7-phosphoheptulonate synthase)
to HMM TIGR01361 (aroF: 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01361.hmm # target sequence database: /tmp/gapView.7767.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01361 [M=260] Accession: TIGR01361 Description: DAHP_synth_Bsub: 3-deoxy-7-phosphoheptulonate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.2e-117 376.1 0.0 5.2e-117 375.8 0.0 1.1 1 lcl|NCBI__GCF_900102855.1:WP_092991507.1 BLP65_RS00420 3-deoxy-7-phosphoh Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_900102855.1:WP_092991507.1 BLP65_RS00420 3-deoxy-7-phosphoheptulonate synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 375.8 0.0 5.2e-117 5.2e-117 3 258 .. 72 328 .. 70 330 .. 0.98 Alignments for each domain: == domain 1 score: 375.8 bits; conditional E-value: 5.2e-117 TIGR01361 3 askkvkkeetvvdvedvkiGegeliviaGPCsveseeqivetakavkeaGakllrGgafkPrtsPysfq 71 ++++++ke+tvvd+ +vk+G++++ viaGPCsve +eq+ ++a++v +aG +l+rGgafkPrtsPysfq lcl|NCBI__GCF_900102855.1:WP_092991507.1 72 VARSYHKEDTVVDIGGVKLGGNTVQVIAGPCSVETQEQMDRAAEMVVAAGCRLMRGGAFKPRTSPYSFQ 140 67899**************************************************************** PP TIGR01361 72 Glgeeglkllkrakdetgllvvtevlderdveivaey.vDilqiGarnmqnfelLkevgkskkPvlLkr 139 G+g egl++ ++a+d++ l++vte++d+r ++ ++ey vD +qiG+rnmqnf+lLkevg+ +kPv+Lkr lcl|NCBI__GCF_900102855.1:WP_092991507.1 141 GHGVEGLEIFRAAADKQKLPIVTELMDVRSLDAFDEYnVDAIQIGTRNMQNFDLLKEVGRIQKPVILKR 209 ************************************978****************************** PP TIGR01361 140 glaatieewleaaeYilsegnenvilcerGirtfekatrftldlsavallkklthlPvivDpshaaGrr 208 g++ati+ewl++aeYi++ gn+n+i+cerGirtfe++ r+ ld++a+++lkk+thlPvivDpsha G+ lcl|NCBI__GCF_900102855.1:WP_092991507.1 210 GMSATIKEWLMSAEYIAAGGNHNIIFCERGIRTFETYYRNVLDVTAIPVLKKETHLPVIVDPSHAGGKA 278 ********************************************************************* PP TIGR01361 209 dlvlplakaavavGadgllievhpdPekalsDseqqltpeefkelvkelk 258 +v++la+aava+Gadgll+e+hp P +a++D++q +tp+e+++l+ el+ lcl|NCBI__GCF_900102855.1:WP_092991507.1 279 WMVPALARAAVAAGADGLLVEMHPSPCEAWCDADQAITPDELNALMGELR 328 **********************************************9876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (260 nodes) Target sequences: 1 (338 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 8.51 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory