Align ketol-acid reductoisomerase [NAD(P)+] (EC 1.1.1.383) (characterized)
to candidate WP_092345545.1 BLU87_RS05460 ketol-acid reductoisomerase
Query= BRENDA::A0KS29 (492 letters) >NCBI__GCF_900107645.1:WP_092345545.1 Length = 490 Score = 707 bits (1826), Expect = 0.0 Identities = 343/487 (70%), Positives = 411/487 (84%), Gaps = 1/487 (0%) Query: 3 NYFNSLNLRQQLAQLGQCRFMDRSEFSDGCNYIKDWNIVILGCGAQGLNQGLNMRDSGLN 62 NYFNSL ++L +LG CRFMD EFS GC Y K IVI+GCGAQGLNQGLNMRDSGL+ Sbjct: 4 NYFNSLPFSRKLQELGTCRFMDADEFSSGCEYAKGKKIVIVGCGAQGLNQGLNMRDSGLD 63 Query: 63 IAYALRPEAIAQKRASWQKATDNGFKVGTFEELIPTADLVLNLTPDKQHSNVVSAVMPLM 122 ++Y LR AIA+KR S+ AT+NGFKVG++EE++P AD+V+NL PDKQH++VV+ V+PLM Sbjct: 64 VSYTLRQGAIAEKRQSYINATENGFKVGSYEEMLPGADIVMNLAPDKQHTDVVNTVVPLM 123 Query: 123 KQGATLSYSHGFNIVEEGMQIRPDITVVMVAPKCPGTEVREEYKRGFGVPTLIAVHPEND 182 ++GAT SY+HGFNIVEEG +IR D+TV+MVAPKCPGTEVREEYKRGFGVPTLIAVH END Sbjct: 124 RKGATFSYAHGFNIVEEGTEIRKDLTVIMVAPKCPGTEVREEYKRGFGVPTLIAVHGEND 183 Query: 183 PNGDGLEIAKAYASATGGDRAGVLQSSFIAEVKSDLMGEQTILCGMLQTGAILGYDKMVA 242 PNGDGLEIAKA SA GGDRAGVL+SSF+AEVKSDLMGEQTILCGMLQ G++L +DKMVA Sbjct: 184 PNGDGLEIAKALCSAQGGDRAGVLESSFVAEVKSDLMGEQTILCGMLQVGSLLCFDKMVA 243 Query: 243 DGVEPGYAAKLIQQGWETVTEALKHGGITNMMDRLSNPAKIKAFEIAEDLKEILQPLFEK 302 DG++ +A KLIQQGWET+TEALKHGGITNM+DRLSNPAK++AF++AE+LKEI++PLFEK Sbjct: 244 DGIDAPWAVKLIQQGWETITEALKHGGITNMLDRLSNPAKMEAFDLAEELKEIMRPLFEK 303 Query: 303 HMDDIISGEFSRTMMQDWANDDANLLRWRAETAETGFENAPVSSEHIDEQTYFDKGIFLV 362 HMDDI+SGEFS+TMM DWANDD NLL+WR ET +T FE + E I EQ YFDKGI +V Sbjct: 304 HMDDILSGEFSQTMMADWANDDKNLLKWREETGQTAFEKTEAAGE-ISEQEYFDKGILMV 362 Query: 363 AMIKAGVELAFDTMVSAGIVEESAYYESLHETPLIANTIARKRLYEMNVVISDTAEYGCY 422 AM+KAGVELAF+TMV +GI ESAYYESLHETPLIANTIARK+LYEMN +ISDTAEYGCY Sbjct: 363 AMVKAGVELAFETMVISGIEPESAYYESLHETPLIANTIARKKLYEMNRIISDTAEYGCY 422 Query: 423 LFNHAAVPMLRDYVNAMSPEYLGAGLKDSSNNVDNLQLIAINDAIRHTSVEYIGAELRGY 482 LF HA VP+L+D++ ++S + +G GL++ N V+N L+ IN IR VE +G LR Sbjct: 423 LFAHACVPLLKDFMASISTDVIGRGLQNQDNQVNNQDLVEINAEIRGHLVEEVGQVLREA 482 Query: 483 MTDMKSI 489 M MK+I Sbjct: 483 MQGMKAI 489 Lambda K H 0.317 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 789 Number of extensions: 20 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 492 Length of database: 490 Length adjustment: 34 Effective length of query: 458 Effective length of database: 456 Effective search space: 208848 Effective search space used: 208848 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
Align candidate WP_092345545.1 BLU87_RS05460 (ketol-acid reductoisomerase)
to HMM TIGR00465 (ilvC: ketol-acid reductoisomerase (EC 1.1.1.86))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00465.hmm # target sequence database: /tmp/gapView.7568.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00465 [M=314] Accession: TIGR00465 Description: ilvC: ketol-acid reductoisomerase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.1e-135 436.1 1.5 4.9e-122 392.8 0.4 2.0 2 lcl|NCBI__GCF_900107645.1:WP_092345545.1 BLU87_RS05460 ketol-acid reducto Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_900107645.1:WP_092345545.1 BLU87_RS05460 ketol-acid reductoisomerase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 392.8 0.4 4.9e-122 4.9e-122 2 312 .. 37 367 .. 36 369 .. 0.98 2 ! 48.6 0.1 2.8e-17 2.8e-17 185 311 .. 361 484 .. 359 487 .. 0.88 Alignments for each domain: == domain 1 score: 392.8 bits; conditional E-value: 4.9e-122 TIGR00465 2 kgkkvaiiGyGsqGeaqalnlrdsglnvivglrkea.....aswkkAeedGfkvltveeaikkadlimi 65 kgkk++i+G+G+qG +q+ln+rdsgl+v+++lr++a +s+ +A+e+Gfkv ++ee+ + ad++m+ lcl|NCBI__GCF_900107645.1:WP_092345545.1 37 KGKKIVIVGCGAQGLNQGLNMRDSGLDVSYTLRQGAiaekrQSYINATENGFKVGSYEEMLPGADIVMN 105 79*********************************9999999*************************** PP TIGR00465 66 LlpDevqkevyeaeikpllkegkallfsHGfnivfkqivipkdvdvvlvAPKgpGalvReeykegrGvp 134 L pD+ q++++++++ pl+++g++++++HGfniv+++ +i+kd++v++vAPK+pG++vReeyk+g+Gvp lcl|NCBI__GCF_900107645.1:WP_092345545.1 106 LAPDK-QHTDVVNTVVPLMRKGATFSYAHGFNIVEEGTEIRKDLTVIMVAPKCPGTEVREEYKRGFGVP 173 *****.99999999******************************************************* PP TIGR00465 135 sliAveqd..vtgeakeiAlayAkaiGgaragvlettFkeEvesDLfGEqavLcGglealikaafdtLv 201 +liAv+ + ++g++ eiA+a+ a Gg ragvle++F++Ev+sDL+GEq++LcG+l+ +++++fd++v lcl|NCBI__GCF_900107645.1:WP_092345545.1 174 TLIAVHGEndPNGDGLEIAKALCSAQGGDRAGVLESSFVAEVKSDLMGEQTILCGMLQVGSLLCFDKMV 242 *****976669********************************************************** PP TIGR00465 202 eaGyqpelAyfeivhelklivdllkekGlelmrdavsntAklgalelreilkeelkkemqkilkeiqnG 270 ++G + A ++i++++++i+++lk++G+++m d++sn+Ak+ a++l+e lke +++ ++k++++i +G lcl|NCBI__GCF_900107645.1:WP_092345545.1 243 ADGIDAPWAVKLIQQGWETITEALKHGGITNMLDRLSNPAKMEAFDLAEELKEIMRPLFEKHMDDILSG 311 ********************************************************************* PP TIGR00465 271 efak....ewalek........eagkpafeearkke..keqeiekvGkelralvka 312 ef++ +wa+++ e+g++afe+++ + +eqe++++G + a+vka lcl|NCBI__GCF_900107645.1:WP_092345545.1 312 EFSQtmmaDWANDDknllkwreETGQTAFEKTEAAGeiSEQEYFDKGILMVAMVKA 367 ***99999*****99***************99988779****************97 PP == domain 2 score: 48.6 bits; conditional E-value: 2.8e-17 TIGR00465 185 LcGglealikaafdtLveaGyqpelAyfeivhelklivdllkekGlelmrdavsntAklgalelreilk 253 ++ +++a++++af+t+v G +pe Ay e +he li++ + +k l +m + +s+tA++g + ++ lcl|NCBI__GCF_900107645.1:WP_092345545.1 361 MVAMVKAGVELAFETMVISGIEPESAYYESLHETPLIANTIARKKLYEMNRIISDTAEYGCYLFAHACV 429 5689**********************************************************9998888 PP TIGR00465 254 eelkkemqkilkeiqn.GefakewalekeagkpafeearkkekeqeiekvGkelralvk 311 lk m i +++ G ++++++ ++ e++ + + +e+vG+ lr+ ++ lcl|NCBI__GCF_900107645.1:WP_092345545.1 430 PLLKDFMASISTDVIGrGLQN----QDNQVNNQDLVEINAEIRGHLVEEVGQVLREAMQ 484 888888888877766313333....578899999****9999*************9886 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (314 nodes) Target sequences: 1 (490 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.05u 0.01s 00:00:00.06 Elapsed: 00:00:00.05 # Mc/sec: 2.77 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory