Align aromatic-amino-acid transaminase (EC 2.6.1.57) (characterized)
to candidate WP_092350344.1 BLU87_RS15100 aspartate/tyrosine/aromatic aminotransferase
Query= BRENDA::P04693 (397 letters) >NCBI__GCF_900107645.1:WP_092350344.1 Length = 396 Score = 347 bits (890), Expect = e-100 Identities = 171/397 (43%), Positives = 249/397 (62%), Gaps = 1/397 (0%) Query: 1 MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIPQLQAVAEAEARLNAQP 60 MF+KV DPIL L E FK DP DK+NLS+G+Y + G P L+ V EAE R+ Q Sbjct: 1 MFEKVQIAPPDPILGLTETFKADPNPDKINLSVGVYQDSSGRTPVLETVKEAEKRILEQE 60 Query: 61 HGASLYLPMEGLNCYRHAIAPLLFGADHPVLKQQRVATIQTLGGSGALKVGADFLKRYFP 120 YL M G Y + LLFGA H ++ +R AT Q GG+GAL+V D+L P Sbjct: 61 DSKG-YLSMTGAPVYCAVVQELLFGAGHEIIDSKRAATAQCPGGTGALRVAGDYLHFVHP 119 Query: 121 ESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRFNDLLATLKTLPARSIVLLHP 180 + +W+S+PTW NH IF AG Y + + TNG+ F+ + ++KT+P ++LLH Sbjct: 120 GAKIWLSNPTWANHNTIFGAAGLTCEQYAYRNPETNGLDFDAMCESIKTIPKGDVILLHG 179 Query: 181 CCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGFGAGMEEDAYAIRAIASAGLPAL 240 CCHNPTG D T +QW + ++L +E++P +D AYQG G+EED + + L Sbjct: 180 CCHNPTGIDPTPEQWAIIGDLLAKQEVLPLVDFAYQGLANGIEEDRTGLLELVKKVKQML 239 Query: 241 VSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQLKATVRRNYSSPPNFGAQVVAAVLN 300 + +SFSK F LY ER G L+++ +DA+ A V+ Q+K +R NYS+PP+ G Q+VA VL+ Sbjct: 240 ICSSFSKNFGLYRERTGALTIVADDAQQANTVMSQVKLRIRYNYSNPPSHGGQIVAVVLS 299 Query: 301 DEALKASWLAEVEEMRTRILAMRQELVKVLSTEMPERNFDYLLNQRGMFSYTGLSAAQVD 360 D+ LKA W EV +R+RI MR VK L + +++F+ ++ QRGMFS++GL+ QV Sbjct: 300 DKELKAKWYKEVAGIRSRINEMRHLFVKTLKEKGVKQDFNSIIEQRGMFSFSGLTKEQVA 359 Query: 361 RLREEFGVYLIASGRMCVAGLNTANVQRVAKAFAAVM 397 RLR+E+ +Y++ SGR+ VAG+ +N+ R+ +A AV+ Sbjct: 360 RLRDEYSIYIVGSGRINVAGMTPSNMDRLCEAIKAVV 396 Lambda K H 0.320 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 440 Number of extensions: 20 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 397 Length of database: 396 Length adjustment: 31 Effective length of query: 366 Effective length of database: 365 Effective search space: 133590 Effective search space used: 133590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory