Align 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase (EC 2.1.1.14) (characterized)
to candidate WP_089323700.1 CHB58_RS08590 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
Query= BRENDA::Q9X112 (734 letters) >NCBI__GCF_900188395.1:WP_089323700.1 Length = 720 Score = 735 bits (1898), Expect = 0.0 Identities = 394/745 (52%), Positives = 514/745 (68%), Gaps = 41/745 (5%) Query: 1 MKAYAFGFPKIGEKREFKKALEDFWKGKITEEQFEEEMNKLRMYMVENYRKNVDVIPSNE 60 +K YA+GFP++G++REFK +E +W GK+TEE+ +E + L + YR VD P E Sbjct: 2 VKTYAYGFPRLGKQREFKTLIESYWSGKLTEEELKEGIKNLNEKREKTYRSYVDAFPQGE 61 Query: 61 LSYYDFVLDTAVMVGAVPERFGEYRG--LSTYFDMARGGKALEMTKFFNTNYHYLVPEIE 118 ++ YD + DTA++ FG Y+ L YF + RG ALEMTK+FNTNYHYLVP+ E Sbjct: 62 MTLYDNLFDTALI-------FGIYKADTLDEYFSLCRGKNALEMTKWFNTNYHYLVPDFE 114 Query: 119 --TEEFYLLENKPLEDYLFFKSKGIETAPWVIGPFTFLYLSKRNGEWIRRPNQMEKLLES 176 +EF + NK E I+ + ++IGPFT L LSK E + LL+S Sbjct: 115 GKEKEFSISWNKHEE---------IKESAYLIGPFTLLKLSKNLPE-----GKFAALLKS 160 Query: 177 LVSVYKEVFEKLVENGCKEILVNEPAFVCDLEKAHWDLILNVYRELSEFP--LTVFTYYD 234 L Y E F+ NG K + ++EPAFV +L + I + Y + + VFTYYD Sbjct: 161 LACKYAEYFKL---NGFKSVHLDEPAFVMELSDKEIEAIKDAYSVFEDIDTKINVFTYYD 217 Query: 235 SVSDYEACVSLPVKRLHFDFVSNE-ENLKNLEKHGFPEDKKLVAGVINGRQPWKVDLRKV 293 SV + + LPV + D V + ENL L+ P+ K L AGVI+GR W+ DL + Sbjct: 218 SVDNLQFVFDLPVAGVGIDLVHDRGENLSQLDSVD-PKGKTLFAGVIDGRNVWRADLFEK 276 Query: 294 ASLVEKLGAS---AISNSCPLFHLPVTLELENNLPGGLKEKLAFAKEKLEELKMLKDFLE 350 A +V+KL ++N+ PLFHLPV L+ LP L K+AFA+EKL+ELK++ D LE Sbjct: 277 AEIVKKLSEKFEVVVTNAAPLFHLPVNLK-GATLPEELVAKVAFAEEKLKELKLISDVLE 335 Query: 351 G---KTFDLPNVSFEDFAVDLQAVERVRNLPEDSFRREKEYTERDRIQRERLNLPLFPTT 407 G K + + F V ERVRNL ++ F + Y ER ++Q+E L LPLFPTT Sbjct: 336 GDDAKAKEWVEGISKAFGVRENVRERVRNLTDEDFNKAVPYAERIKLQQEILKLPLFPTT 395 Query: 408 TIGSFPQTPEVRKMRSKYRKGEISKEEYEAFIKEQIKKAIELQEEIGLDVLVHGEFERTD 467 TIGSFPQT EVR++R RKG+++ E+Y+ FIK +I KAI++QEE+G+DV VHGEFER+D Sbjct: 396 TIGSFPQTQEVRRVRLLNRKGQLADEDYKTFIKGEIAKAIKIQEELGVDVFVHGEFERSD 455 Query: 468 MVEFFAEKLNGIATTQNGWVLSYGSRCYRPPIIYGTVTRPEPMTLKEITYAQSLTEKPVK 527 MVEFFAEKL GIATT NGWV+SYG+R YRPPII+G V R EPMT++EI +AQSLT+KPVK Sbjct: 456 MVEFFAEKLEGIATTGNGWVISYGTRSYRPPIIFGDVERIEPMTVEEIAFAQSLTDKPVK 515 Query: 528 GMLTGPVTIMSWSYYREDIPEREIAYQIALAINEEVKDLEEAGIKIVQIDEPAFREKAPI 587 GMLTGPVTI++WSY REDIP E+AYQI LA+ +E+ D E+AGIKIVQIDE AFREKAPI Sbjct: 516 GMLTGPVTIIAWSYCREDIPVSEVAYQIGLALRDEIADYEKAGIKIVQIDEAAFREKAPI 575 Query: 588 KKSKWPEYFEWAINAFNLA-ANARPETQIHAHMCYSDFNEIIEYIHQLEFDVISIEASRS 646 KK W EYF+WA+ +F + A ++PETQIH+HMCYS+F EIIEYI ++FDVISIEASRS Sbjct: 576 KKRNWDEYFDWAVKSFRVCHAKSKPETQIHSHMCYSEFGEIIEYILAMDFDVISIEASRS 635 Query: 647 KGEIISAFENFKGWIKQIGVGVWDIHSPAVPSINEMREIVERVLRVLPKELIWINPDCGL 706 KG+II AFE + +QIG+GVWDIHSP VPS++EM+EIVER L+V+PKE W+NPDCGL Sbjct: 636 KGDIIEAFEKV-NFDRQIGLGVWDIHSPYVPSVDEMKEIVERALKVIPKENFWVNPDCGL 694 Query: 707 KTRNWDEVIPSLRNMVALAKEMREK 731 KTR W+E IP++RN+V LA +RE+ Sbjct: 695 KTRRWEECIPAIRNIVKLANTLREE 719 Lambda K H 0.318 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1557 Number of extensions: 74 Number of successful extensions: 13 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 734 Length of database: 720 Length adjustment: 40 Effective length of query: 694 Effective length of database: 680 Effective search space: 471920 Effective search space used: 471920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory