Align Anthranilate synthase component 2; AS; ASII; EC 4.1.3.27; Anthranilate synthase, GATase component; Anthranilate synthase, glutamine amidotransferase component (uncharacterized)
to candidate N515DRAFT_2714 N515DRAFT_2714 carbamoyl-phosphate synthase small subunit
Query= curated2:P9WN34 (232 letters) >FitnessBrowser__Dyella79:N515DRAFT_2714 Length = 375 Score = 61.6 bits (148), Expect = 2e-14 Identities = 45/138 (32%), Positives = 62/138 (44%), Gaps = 9/138 (6%) Query: 48 DGVLLSPGPGTPERAGASVSIVHACAAAHTPLLGVCLGHQAIGVAFGATVDRAPELLHGK 107 DGV LS GPG P +V+ A PL G+CLGHQ +G+A GA + HG Sbjct: 231 DGVFLSNGPGDPAACDYAVAATREFLDAKIPLFGICLGHQIMGLALGAKTLKMKFGHHGA 290 Query: 108 TSSVFHTNVGVLQGLPDPFTATRYHSLTILPKSLPAVLRVT-ARTSSGVIMAVQHTGLPI 166 V + G + ++ H + P +LPA +RVT + G + T P Sbjct: 291 NHPVKDHDDGRV------LITSQNHGFAVDPATLPANVRVTHSSLFDGSLQGFALTDRPA 344 Query: 167 HGVQFHPESILTEGGHRI 184 Q HPE+ + G H I Sbjct: 345 FCFQGHPEA--SPGPHDI 360 Lambda K H 0.320 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 228 Number of extensions: 5 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 232 Length of database: 375 Length adjustment: 26 Effective length of query: 206 Effective length of database: 349 Effective search space: 71894 Effective search space used: 71894 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory