GapMind for Amino acid biosynthesis


L-proline biosynthesis in Pseudomonas fluorescens FW300-N1B4

Best path

argA, argB, argC, argD, argE, cyclodeaminase

Also see fitness data for the top candidates


Overview: Proline biosynthesis in GapMind is based on MetaCyc pathways L-proline biosynthesis I from L-glutamate (link) and III via L-ornithine aminotransferase (link). GapMind also describes proline synthesis via ornithine cyclodeaminase (PMID:11489875). Pathway II from arginine (link) should perhaps be included, but is not; it is reported in nitrogen-fixing cyanobacteria and the bacteria it was reported in also have pathway I. Pathway IV is not included because it is not reported in prokaryotes (link). Also, pathway III is described in MetaCyc as occuring only in plants, but it has been reported in the archaeon Thermococcus kodakarensis (PMC5846162).

17 steps (15 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
argA N-acylglutamate synthase Pf1N1B4_2243 Pf1N1B4_656
argB N-acylglutamate kinase Pf1N1B4_2121 Pf1N1B4_2243
argC N-acylglutamylphosphate reductase Pf1N1B4_2559
argD N-acetylornithine aminotransferase Pf1N1B4_3440 Pf1N1B4_1733
argE N-acetylornithine deacetylase Pf1N1B4_2242 Pf1N1B4_4930
cyclodeaminase ornithine cyclodeaminase Pf1N1B4_4977
Alternative steps:
argJ ornithine acetyltransferase Pf1N1B4_656
argX glutamate--LysW ligase Pf1N1B4_1774
lysJ [LysW]-glutamate-semialdehyde aminotransferase Pf1N1B4_205 Pf1N1B4_3440
lysK [LysW]-ornithine hydrolase
lysW 2-aminoadipate/glutamate carrier protein
lysY [LysW]-glutamate-6-phosphate reductase Pf1N1B4_2559
lysZ [LysW]-glutamate kinase Pf1N1B4_2121
OAT L-ornithine 5-aminotransferase Pf1N1B4_2980 Pf1N1B4_4910
proA gamma-glutamylphosphate reductase Pf1N1B4_2688 Pf1N1B4_4438
proB glutamate 5-kinase Pf1N1B4_2800 Pf1N1B4_2688
proC pyrroline-5-carboxylate reductase Pf1N1B4_2341

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.



Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory