GapMind for Amino acid biosynthesis

 

L-proline biosynthesis in Azoarcus olearius BH72

Best path

argJ, argB, argC, argD, OAT, proC

Rules

Overview: Proline biosynthesis in GapMind is based on MetaCyc pathways L-proline biosynthesis I from L-glutamate (link) and III via L-ornithine aminotransferase (link). GapMind also describes proline synthesis via ornithine cyclodeaminase (PMID:11489875). Pathway II from arginine (link) should perhaps be included, but is not; it is reported in nitrogen-fixing cyanobacteria and the bacteria it was reported in also have pathway I. Pathway IV is not included because it is not reported in prokaryotes (link). Also, pathway III is described in MetaCyc as occuring only in plants, but it has been reported in the archaeon Thermococcus kodakarensis (PMC5846162).

17 steps (12 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
argJ ornithine acetyltransferase AZO_RS04505
argB N-acylglutamate kinase AZO_RS16300 AZO_RS14225
argC N-acylglutamylphosphate reductase AZO_RS19650
argD N-acetylornithine aminotransferase AZO_RS11060 AZO_RS06045
OAT L-ornithine 5-aminotransferase AZO_RS21240 AZO_RS06045
proC pyrroline-5-carboxylate reductase AZO_RS17410
Alternative steps:
argA N-acylglutamate synthase AZO_RS14225 AZO_RS04505
argE N-acetylornithine deacetylase AZO_RS01055 AZO_RS08110
argX glutamate--LysW ligase
cyclodeaminase ornithine cyclodeaminase
lysJ [LysW]-glutamate-semialdehyde aminotransferase AZO_RS11060 AZO_RS21240
lysK [LysW]-ornithine hydrolase
lysW 2-aminoadipate/glutamate carrier protein
lysY [LysW]-glutamate-6-phosphate reductase
lysZ [LysW]-glutamate kinase AZO_RS16300
proA gamma-glutamylphosphate reductase AZO_RS16170 AZO_RS15905
proB glutamate 5-kinase AZO_RS15905 AZO_RS16170

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory