Alignments for a candidate for ilvI in Sulfuritalea hydrogenivorans DSM 22779

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Sulfuritalea hydrogenivorans DSM 22779

Align Acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized)
to candidate WP_171817373.1 SUTH_RS13830 thiamine pyrophosphate-binding protein

Query= curated2:Q7U5G1
         (617 letters)



>NCBI__GCF_000828635.1:WP_171817373.1
          Length = 602

 Score =  273 bits (699), Expect = 1e-77
 Identities = 201/577 (34%), Positives = 293/577 (50%), Gaps = 31/577 (5%)

Query: 27  LMDALRRHGVDTIFGYPGGAILPIYDALHIAESEGWVKHILVRHEQAGTHAADAYARATG 86
           LM  L   G+D +F  PGG  + + D L     E  +  I   HEQA   AA+A+ R  G
Sbjct: 7   LMSHLAGLGIDHVFVLPGGGAMHLNDGL---ACEPRITAIPCHHEQACGIAAEAWGRVAG 63

Query: 87  KVGVCFGTSGPGATNLVTGIATAQMDSVPMVVITGQVPRPAIGTD------AFQETDIFG 140
           K GV   T+GPGATN+VT +  A ++SVPM+VI+GQV RP +  D        QE DI  
Sbjct: 64  KFGVAMVTTGPGATNVVTPVTGAWIESVPMLVISGQVKRPDLLRDRPLRQGGVQEVDIVP 123

Query: 141 ITLPIVKHSWVVRDPADLGSIVAQAFLIAASGRPGPVLIDIPKDVGQEQFNYVPVEPGSV 200
           I   I K++  V+DPA +   + +A      GR GPV +DIP DV   Q   VP E    
Sbjct: 124 IVRSITKYAVTVQDPATIRYHLERALHQMQDGRAGPVWLDIPLDVQAAQI--VPAELTGY 181

Query: 201 IPGGFHQPEPPLDAAVAAALDLIEQAQRPLLYVGGGAISACAHDSLRMLAERYQLPVTTT 260
           IP    + +P LD  +   L+LI  A+RPL+  G G   A A      LAE+  +PV TT
Sbjct: 182 IPEA--EVQPGLDEPIQQTLELIAAAERPLILAGHGVRLAGAAKRFLALAEKLDIPVVTT 239

Query: 261 LMGKGAFDENDALSVGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTGKLDT-FAPRAR 319
                       L VG  G+     ANFAV  CDLLI++G R D+ +T      FA  AR
Sbjct: 240 WNALDLIPWAHPLCVGRPGVVALRAANFAVQNCDLLISIGCRLDNIITAYNPRGFARTAR 299

Query: 320 VVHFEIDPAEIGK-NRKADVAVLGDLGLSLARMVEISLQRTAEPRTAAWLERINTWKDRY 378
            +  ++DP EI K +   D+ +  D G S    +      ++ P   AW +R + WK RY
Sbjct: 300 KIVVDVDPEEIAKLDMDIDLRLPVDAG-SFIDALAAHTGSSSLPDRPAWRQRCSDWKRRY 358

Query: 379 PLTIPP---AEGAIYPQEVLLAVRDLAP-DAIVTTD---VGQHQMWAAQHLRNGPRGWIS 431
            +        +G I     + A+ D AP + +V+T    +G    +     + G R +++
Sbjct: 359 AVGEGKPFHTDGPISHYHFVSALSDAAPPETLVSTGSSGLGVEAFYTVFRNKPGQRVFLT 418

Query: 432 SAGLGTMGFGMPAAMGAQVAMPDRQVVCIAGDASILMNIQELGTLAAYGLPVKVVIVNNH 491
           S GLG MG+G+PAA+GA +A   R  + I  D S+ +N+QEL T+ A  LP+ +VI++N 
Sbjct: 419 S-GLGAMGYGLPAAIGACLAGNRRPAIAIESDGSLQLNLQELATVKAQKLPICLVIMDNG 477

Query: 492 WQGMVRQWQESFYDERYSASDMLNG--MPDFIALARSFGVDGVKITDRELLHRDLAAALQ 549
               +R  Q +++ ERY   D  +G  +PD   +A ++G+  ++I D   +   L  AL 
Sbjct: 478 GYCSIRNTQRNYFQERYLGIDPASGLWLPDLEKIAAAYGLPFLRIDDPGNIDAALKEALA 537

Query: 550 SPTPTMIDVHVRRGENCYP---MVPPGKSNAQMVGLP 583
            P P +IDV + + E   P    VP  + +  M+ +P
Sbjct: 538 LPRPCLIDVRLMKDETLAPKSAAVP--QPDGSMISMP 572


Lambda     K      H
   0.320    0.136    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 981
Number of extensions: 53
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 617
Length of database: 602
Length adjustment: 37
Effective length of query: 580
Effective length of database: 565
Effective search space:   327700
Effective search space used:   327700
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis