Align dihydroxyacid dehydratase (characterized)
to candidate 7023120 Shewana3_0358 dihydroxy-acid dehydratase (RefSeq)
Query= CharProtDB::CH_024768 (616 letters) >FitnessBrowser__ANA3:7023120 Length = 619 Score = 983 bits (2540), Expect = 0.0 Identities = 493/617 (79%), Positives = 543/617 (88%), Gaps = 3/617 (0%) Query: 1 MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFTQFVPGHVHLRDLGKLV 60 MPK RSAT+T GRNMAGARALWRATG+ D DFGKPIIA+ NSFTQFVPGHVHL+D+G LV Sbjct: 1 MPKLRSATSTEGRNMAGARALWRATGVKDNDFGKPIIAIANSFTQFVPGHVHLKDMGSLV 60 Query: 61 AEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS 120 A+ IE AGG+AKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADA+VCIS Sbjct: 61 ADAIEEAGGIAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADALVCIS 120 Query: 121 NCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQIIKLDLVDAMIQGADPKVSDSQ 180 NCDKITPGMLMA+LRLNIPV+FVSGGPMEAGKTKLSD++IKLDLVDAM+ AD VSD Sbjct: 121 NCDKITPGMLMAALRLNIPVVFVSGGPMEAGKTKLSDKLIKLDLVDAMVAAADSSVSDED 180 Query: 181 SDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLNAGKRIV 240 S ++ERSACPTCGSCSGMFTANSMNCLTEALGLS PGNGS+LATHADR++LFL AG+R++ Sbjct: 181 SAKIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSMLATHADRRELFLEAGRRVM 240 Query: 241 ELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSD 300 LTKRYYEQ+D SALPRNIAS AFENAM LDIAMGGS+NTVLHLLAAAQEA++ FTM D Sbjct: 241 ALTKRYYEQDDASALPRNIASFKAFENAMALDIAMGGSSNTVLHLLAAAQEADVAFTMDD 300 Query: 301 IDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIGILGELDRAGLLNRDVKNV---LGLTL 357 ID++SR+VP LCKVAPST KYHMEDVHRAGGV+GILGELDRAGLL+ DV +V G L Sbjct: 301 IDRMSRQVPHLCKVAPSTAKYHMEDVHRAGGVMGILGELDRAGLLHTDVPHVAADAGGNL 360 Query: 358 PQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFSQDCRWDTLDDDRANGCIRSLEHAYS 417 L +YDVM TQDD VK F AGPAGI TT+AFSQDCRW +LDDDR GCIRS E A+S Sbjct: 361 KSVLAKYDVMQTQDDKVKQFFMAGPAGIPTTKAFSQDCRWPSLDDDRREGCIRSREFAFS 420 Query: 418 KDGGLAVLYGNFAENGCIVKTAGVDDSILKFTGPAKVYESQDDAVEAILGGKVVAGDVVV 477 ++GGLAVL GN A+NGCIVKTAGVD+S L F G A+VYESQDDAV ILGG+VVAGDVVV Sbjct: 421 QEGGLAVLSGNLADNGCIVKTAGVDESNLTFIGSARVYESQDDAVAGILGGEVVAGDVVV 480 Query: 478 IRYEGPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGS 537 IRYEGPKGGPGMQEMLYPTS+LKS GLGKACALITDGRFSGGTSGLSIGHVSPEAA+GG+ Sbjct: 481 IRYEGPKGGPGMQEMLYPTSYLKSRGLGKACALITDGRFSGGTSGLSIGHVSPEAAAGGT 540 Query: 538 IGLIEDGDLIAIDIPNRGIQLQVSDAELAARREAQDARGDKAWTPKNRERQVSFALRAYA 597 I LIE+GD I IDIP R I+L VSDAELAARRE ARG AW P +R+R VS AL+AYA Sbjct: 541 IALIENGDRIEIDIPKRSIKLAVSDAELAARRETMLARGPMAWKPLSRQRYVSMALKAYA 600 Query: 598 SLATSADKGAVRDKSKL 614 LATSADKGAVRD+SKL Sbjct: 601 MLATSADKGAVRDRSKL 617 Lambda K H 0.317 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1306 Number of extensions: 49 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 616 Length of database: 619 Length adjustment: 37 Effective length of query: 579 Effective length of database: 582 Effective search space: 336978 Effective search space used: 336978 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate 7023120 Shewana3_0358 (dihydroxy-acid dehydratase (RefSeq))
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.12355.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.5e-258 844.0 4.1 2.9e-258 843.8 4.1 1.0 1 lcl|FitnessBrowser__ANA3:7023120 Shewana3_0358 dihydroxy-acid deh Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__ANA3:7023120 Shewana3_0358 dihydroxy-acid dehydratase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 843.8 4.1 2.9e-258 2.9e-258 2 542 .. 18 613 .. 17 614 .. 0.98 Alignments for each domain: == domain 1 score: 843.8 bits; conditional E-value: 2.9e-258 TIGR00110 2 arallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiamgheGmkysL 78 aral++atG+kd+d++kPiia++ns+t++vPghvhlkd+++lv+++ie+aGg+akefntiav+DGiamgh+Gm+ysL lcl|FitnessBrowser__ANA3:7023120 18 ARALWRATGVKDNDFGKPIIAIANSFTQFVPGHVHLKDMGSLVADAIEEAGGIAKEFNTIAVDDGIAMGHGGMLYSL 94 79*************************************************************************** PP TIGR00110 79 psreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktklsekidlvdvfeavgey 155 psre+iaDsve++v+ah++Dalv+is+CDki+PGmlmaalrlniP+++vsGGpmeagktkls+k++++d+++a++++ lcl|FitnessBrowser__ANA3:7023120 95 PSRELIADSVEYMVNAHCADALVCISNCDKITPGMLMAALRLNIPVVFVSGGPMEAGKTKLSDKLIKLDLVDAMVAA 171 ***************************************************************************** PP TIGR00110 156 aagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstllatsaekkelakksgkrivelvkknik 232 a++ +s+e++ +iersacPt+gsCsG+ftansm+cltealGlslPg++++lat+a+++el+ ++g+r++ l+k++++ lcl|FitnessBrowser__ANA3:7023120 172 ADSSVSDEDSAKIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSMLATHADRRELFLEAGRRVMALTKRYYE 248 ***************************************************************************** PP TIGR00110 233 .......PrdiltkeafenaitldlalGGstntvLhllaiakeagvklslddfdrlsrkvPllaklkPsgkkv.ied 301 Pr+i++ +afena++ld+a+GGs+ntvLhlla+a+ea+v +++dd+dr+sr+vP+l+k++Ps+ k+ +ed lcl|FitnessBrowser__ANA3:7023120 249 qddasalPRNIASFKAFENAMALDIAMGGSSNTVLHLLAAAQEADVAFTMDDIDRMSRQVPHLCKVAPSTAKYhMED 325 **********************************************************************9999*** PP TIGR00110 302 lhraGGvsavlkeldkegllhkdaltvt...Gktlaetlekvkvlr............................... 344 +hraGGv+++l+eld++gllh+d+ v+ G l+ +l+k++v++ lcl|FitnessBrowser__ANA3:7023120 326 VHRAGGVMGILGELDRAGLLHTDVPHVAadaGGNLKSVLAKYDVMQtqddkvkqffmagpagipttkafsqdcrwps 402 ***********************99985333889***********9******************************9 PP TIGR00110 345 vdqd....virsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvkeGdvv 417 +d+d +irs + ++++egglavL Gnla++G++vk+agv+e+ l+f G+a+v+es+++a+++ilgg+v +Gdvv lcl|FitnessBrowser__ANA3:7023120 403 LDDDrregCIRSREFAFSQEGGLAVLSGNLADNGCIVKTAGVDESNLTFIGSARVYESQDDAVAGILGGEVVAGDVV 479 44444566********************************************************************* PP TIGR00110 418 viryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialvedGDkikiDien 494 viryeGPkGgPGm+emL+Pts+l++ GLgk++aLitDGrfsGgt+GlsiGhvsPeaa+gG+ial+e+GD+i+iDi++ lcl|FitnessBrowser__ANA3:7023120 480 VIRYEGPKGGPGMQEMLYPTSYLKSRGLGKACALITDGRFSGGTSGLSIGHVSPEAAAGGTIALIENGDRIEIDIPK 556 ***************************************************************************** PP TIGR00110 495 rkldlevseeelaerrakakkkea.........revkgaLakyaklvssadkGavld 542 r ++l+vs++ela+rr+++ ++++ r+v+ aL++ya l++sadkGav+d lcl|FitnessBrowser__ANA3:7023120 557 RSIKLAVSDAELAARRETMLARGPmawkplsrqRYVSMALKAYAMLATSADKGAVRD 613 **********************9999*****************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (619 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 11.95 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory