Align 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase; Tetrahydrodipicolinate N-acetyltransferase; THP acetyltransferase; Tetrahydropicolinate acetylase; EC 2.3.1.89 (characterized)
to candidate 7023084 Shewana3_0322 antibiotic acetyltransferase (RefSeq)
Query= SwissProt::O34981 (236 letters) >FitnessBrowser__ANA3:7023084 Length = 215 Score = 57.8 bits (138), Expect = 2e-13 Identities = 24/50 (48%), Positives = 37/50 (74%) Query: 168 IEDDVVIGANAVVLEGVTVGKGAVVAAGAIVVNDVEPYTVVAGTPAKKIK 217 I D +G A+++ GV++G+GA+VAA ++V DV PY++V GTPAK +K Sbjct: 114 IGDGAWLGMRAMIMPGVSIGEGAIVAANSVVTQDVAPYSIVGGTPAKLVK 163 Score = 27.7 bits (60), Expect = 2e-04 Identities = 16/43 (37%), Positives = 22/43 (51%) Query: 94 IEPGAIIRDQVEIGDNAVIMMGASINIGSVIGEGTMIDMNVVL 136 IE + + IGD A + M A I G IGEG ++ N V+ Sbjct: 102 IEEAYVGKGDTHIGDGAWLGMRAMIMPGVSIGEGAIVAANSVV 144 Lambda K H 0.313 0.134 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 131 Number of extensions: 12 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 236 Length of database: 215 Length adjustment: 22 Effective length of query: 214 Effective length of database: 193 Effective search space: 41302 Effective search space used: 41302 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory