Alignments for a candidate for lysA in Shewanella sp. ANA-3

GapMind for Amino acid biosynthesis

Alignments for a candidate for lysA in Shewanella sp. ANA-3

Align diaminopimelate decarboxylase (EC 4.1.1.20) (characterized)
to candidate 7023156 Shewana3_0390 diaminopimelate decarboxylase (RefSeq)

Query= BRENDA::Q9KVL7
         (417 letters)



>FitnessBrowser__ANA3:7023156
          Length = 414

 Score =  581 bits (1497), Expect = e-170
 Identities = 290/416 (69%), Positives = 333/416 (80%), Gaps = 2/416 (0%)

Query: 1   MDYFNYQEDGQLWAEQVPLADLANQYGTPLYVYSRATLERHWHAFDKSVGDYPHLICYAV 60
           MD+F YQ D  L+AE   + DLA QYGTPLY+YSRATLERHWHAF+ +V D+PHL+CYAV
Sbjct: 1   MDHFLYQ-DNTLYAEGCRVNDLAQQYGTPLYIYSRATLERHWHAFNNAVADHPHLVCYAV 59

Query: 61  KANSNLGVLNTLARLGSGFDIVSVGELERVLAAGGDPSKVVFSGVGKTEAEMKRALQLKI 120
           KANSNL VLN LARLGSGFDIVS GEL RV+ AGGDP+KVVFSGVGKT AEM++AL L I
Sbjct: 60  KANSNLAVLNVLARLGSGFDIVSGGELARVIEAGGDPAKVVFSGVGKTVAEMEQALNLGI 119

Query: 121 KCFNVESEPELQRLNKVAGELGVKAPISLRINPDVDAKTHPYISTGLRDNKFGITFDRAA 180
            CFNVES  EL++LN VAG LG  AP+SLR+NPDVDA THPYISTGL++NKFGI  D A 
Sbjct: 120 YCFNVESSAELEQLNDVAGRLGKVAPVSLRVNPDVDAGTHPYISTGLKENKFGIAMDEAE 179

Query: 181 QVYRLAHSLPNLDVHGIDCHIGSQLTALAPFIDATDRLLALIDSLKAEGIHIRHLDVGGG 240
            V+  AH+LP+L V G+DCHIGSQLT + PF+DA DR+LALID L  +GI I H DVGGG
Sbjct: 180 VVFARAHALPHLQVKGVDCHIGSQLTEIQPFLDAMDRMLALIDRLADQGIVIEHFDVGGG 239

Query: 241 LGVVYRDELPPQPSEYAKALLDRLERHRDLELIFEPGRAIAANAGVLVTKVEFLKHTEHK 300
           LGV Y DE PP P  YA ALL RL   R L+LIFEPGRAIAANAG+ VT+V +LK    K
Sbjct: 240 LGVTYDDETPPHPDVYAAALLARL-GDRKLKLIFEPGRAIAANAGIFVTQVLYLKENSDK 298

Query: 301 NFAIIDAAMNDLIRPALYQAWQDIIPLRPRQGEAQTYDLVGPVCETSDFLGKDRDLVLQE 360
            FAI+D AMNDLIRPALY AWQ+IIP+ PR  +AQ +D+VGPVCET DFLGKDR L +  
Sbjct: 299 RFAIVDGAMNDLIRPALYSAWQNIIPVNPRDEQAQVFDIVGPVCETGDFLGKDRALAIAA 358

Query: 361 GDLLAVRSSGAYGFTMSSNYNTRPRVAEVMVDGNKTYLVRQREELSSLWALESVLP 416
           GDLL VRSSGAYGF M+SNYNTRPR AEVMVDG+K YLVR+RE+L+ LW  E +LP
Sbjct: 359 GDLLVVRSSGAYGFAMASNYNTRPRAAEVMVDGDKAYLVREREKLAQLWQGEQLLP 414


Lambda     K      H
   0.319    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 522
Number of extensions: 15
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 417
Length of database: 414
Length adjustment: 31
Effective length of query: 386
Effective length of database: 383
Effective search space:   147838
Effective search space used:   147838
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate 7023156 Shewana3_0390 (diaminopimelate decarboxylase (RefSeq))
to HMM TIGR01048 (lysA: diaminopimelate decarboxylase (EC 4.1.1.20))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01048.hmm
# target sequence database:        /tmp/gapView.31422.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01048  [M=417]
Accession:   TIGR01048
Description: lysA: diaminopimelate decarboxylase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
     2e-165  536.3   0.0   2.4e-165  536.0   0.0    1.0  1  lcl|FitnessBrowser__ANA3:7023156  Shewana3_0390 diaminopimelate de


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__ANA3:7023156  Shewana3_0390 diaminopimelate decarboxylase (RefSeq)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  536.0   0.0  2.4e-165  2.4e-165       5     416 ..       7     410 ..       4     411 .. 0.98

  Alignments for each domain:
  == domain 1  score: 536.0 bits;  conditional E-value: 2.4e-165
                         TIGR01048   5 kdgeleiegvdlkelaeefgtPlYvydeetlrerlealkeafkaeeslvlYAvKAnsnlavlrllaeeGlgldvvsg 81 
                                       +d +l++eg+++++la+++gtPlY+y+++tl+++++a+++a++++ +lv+YAvKAnsnlavl++la++G+g+d+vsg
  lcl|FitnessBrowser__ANA3:7023156   7 QDNTLYAEGCRVNDLAQQYGTPLYIYSRATLERHWHAFNNAVADHPHLVCYAVKANSNLAVLNVLARLGSGFDIVSG 83 
                                       677899*********************************************************************** PP

                         TIGR01048  82 GEleralaAgvkaekivfsgngkseeeleaaleleiklinvdsveelelleeiakelgkkarvllRvnpdvdakthe 158
                                       GEl+r+ +Ag ++ k+vfsg+gk+ +e+e+al+l+i ++nv+s +ele+l+++a++lgk a+v+lRvnpdvda th+
  lcl|FitnessBrowser__ANA3:7023156  84 GELARVIEAGGDPAKVVFSGVGKTVAEMEQALNLGIYCFNVESSAELEQLNDVAGRLGKVAPVSLRVNPDVDAGTHP 160
                                       ***************************************************************************** PP

                         TIGR01048 159 yisTGlkesKFGieveeaeeayelalkleslelvGihvHIGSqildlepfveaaekvvklleelkeegieleeldlG 235
                                       yisTGlke+KFGi+++eae ++ +a++l++l++ G+++HIGSq+++++pf +a+++++ l+++l+++gi +e++d+G
  lcl|FitnessBrowser__ANA3:7023156 161 YISTGLKENKFGIAMDEAEVVFARAHALPHLQVKGVDCHIGSQLTEIQPFLDAMDRMLALIDRLADQGIVIEHFDVG 237
                                       ***************************************************************************** PP

                         TIGR01048 236 GGlgisyeeeeeapdleeyaeklleklekeaelglklklilEpGRslvanagvlltrVesvKevesrkfvlvDagmn 312
                                       GGlg++y++e+ +p+++ ya++ll++l +     +klkli+EpGR+++anag+++t+V ++Ke++ ++f++vD++mn
  lcl|FitnessBrowser__ANA3:7023156 238 GGLGVTYDDET-PPHPDVYAAALLARLGD-----RKLKLIFEPGRAIAANAGIFVTQVLYLKENSDKRFAIVDGAMN 308
                                       *********99.***************99.....69***************************************** PP

                         TIGR01048 313 dliRpalYeayheiaalkrleeeetetvdvvGplCEsgDvlakdrelpeveeGdllavasaGAYgasmssnYnsrpr 389
                                       dliRpalY+a+++i+++ + ++e+++++d+vGp+CE+gD+l+kdr+l  +  Gdll+v+s+GAYg++m+snYn+rpr
  lcl|FitnessBrowser__ANA3:7023156 309 DLIRPALYSAWQNIIPV-NPRDEQAQVFDIVGPVCETGDFLGKDRALAIAA-GDLLVVRSSGAYGFAMASNYNTRPR 383
                                       *****************.568888***********************9877.************************* PP

                         TIGR01048 390 paevlveegkarlirrretledllale 416
                                       +aev+v+++ka+l+r+re+l++l++ e
  lcl|FitnessBrowser__ANA3:7023156 384 AAEVMVDGDKAYLVREREKLAQLWQGE 410
                                       ************************976 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (417 nodes)
Target sequences:                          1  (414 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 7.63
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis