Aligments for a candidate for aroE in Burkholderia phytofirmans PsJN

GapMind for Amino acid biosynthesis

Aligments for a candidate for aroE in Burkholderia phytofirmans PsJN

Align shikimate dehydrogenase (EC 1.1.1.25) (characterized)
to candidate BPHYT_RS16975 BPHYT_RS16975 shikimate 5-dehydrogenase

Query= BRENDA::Q88RQ5
         (274 letters)



>FitnessBrowser__BFirm:BPHYT_RS16975
          Length = 287

 Score =  265 bits (678), Expect = 6e-76
 Identities = 154/277 (55%), Positives = 179/277 (64%), Gaps = 10/277 (3%)

Query: 2   DQYVVFGNPIGHSKSPLIHRLFAEQTGQDLEYATLLAPLDEFSDCARGFFKQGSGG-NVT 60
           D+Y V GNP+GHSKSP IH  FA QTG+ +EY  LLAP+D F    R F   G  G NVT
Sbjct: 8   DRYAVIGNPVGHSKSPFIHGRFAAQTGEAVEYGHLLAPVDAFVPHVRAFIDAGGRGLNVT 67

Query: 61  VPFKEEAFRLCDSLTPRARRAGAVNTLSKLADGTLQGDNTDGAGLVRDLTVNAGVELAGK 120
           VPFK +A    D+L+PRA  AGAVNTL   A+G + GDNTDG GLVRD+ VN GV L G 
Sbjct: 68  VPFKLDAHAFADTLSPRAAAAGAVNTLRFDANG-IYGDNTDGFGLVRDIEVNLGVSLKGA 126

Query: 121 RILILGAGGAVRGVLEPILAHKPQSLVIANRTVEKAEQLAREFDELGPVVA------SGF 174
           RIL+LGAGGA RGV+ P+L   P +L I NRT EKAE L  +F +     A      S  
Sbjct: 127 RILLLGAGGAARGVVLPMLDRAPHTLTIVNRTAEKAEALVAQFAQAARDAACQLSGGSAR 186

Query: 175 AWLQEPVDVIINATSASLAGELPPIADSLVEAGRTVCYDMMYGKEPTPFCQWATKLGAAK 234
           A      DVI+NAT+ SL   LP   D     G T+ YDMMYG  PT F + A KLG A+
Sbjct: 187 AIEAGTYDVIVNATAGSLDASLPECDDRAFGNG-TLAYDMMYGAHPTVFMEHARKLG-AR 244

Query: 235 VLDGLGMLAEQAAEAFFIWRGVRPDTAPVLAELRRQL 271
             DGLGML EQAAE+F++WRGVRPD APVLAELR  L
Sbjct: 245 GADGLGMLVEQAAESFYVWRGVRPDGAPVLAELRALL 281


Lambda     K      H
   0.319    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 264
Number of extensions: 13
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 274
Length of database: 287
Length adjustment: 26
Effective length of query: 248
Effective length of database: 261
Effective search space:    64728
Effective search space used:    64728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate BPHYT_RS16975 BPHYT_RS16975 (shikimate 5-dehydrogenase)
to HMM TIGR00507 (aroE: shikimate dehydrogenase (EC 1.1.1.25))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00507.hmm
# target sequence database:        /tmp/gapView.17746.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00507  [M=270]
Accession:   TIGR00507
Description: aroE: shikimate dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                -----------
      2e-78  249.2   0.0    2.3e-78  249.1   0.0    1.0  1  lcl|FitnessBrowser__BFirm:BPHYT_RS16975  BPHYT_RS16975 shikimate 5-dehydr


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__BFirm:BPHYT_RS16975  BPHYT_RS16975 shikimate 5-dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  249.1   0.0   2.3e-78   2.3e-78       3     267 ..      10     279 ..       8     282 .. 0.92

  Alignments for each domain:
  == domain 1  score: 249.1 bits;  conditional E-value: 2.3e-78
                                TIGR00507   3 lgviGnpikhSksplihnaalkqlgleleYlafeveieelekalsgikalglkGvnvTvPfKeevlellD 72 
                                              ++viGnp+ hSksp+ih ++++q+g ++eY ++ ++++++   + ++  +g +G+nvTvPfK  + +++D
  lcl|FitnessBrowser__BFirm:BPHYT_RS16975  10 YAVIGNPVGHSKSPFIHGRFAAQTGEAVEYGHLLAPVDAFVPHVRAFIDAGGRGLNVTVPFKLDAHAFAD 79 
                                              9********************************************************************* PP

                                TIGR00507  73 eieesakligavNTlkledgklvgynTDgiGlvssLek.lsklksekrvliiGAGGaakavaleLlka.d 140
                                               ++++a+++gavNTl+ + + ++g+nTDg Glv ++e  l    ++ r+l++GAGGaa++v+l++l+  +
  lcl|FitnessBrowser__BFirm:BPHYT_RS16975  80 TLSPRAAAAGAVNTLRFDANGIYGDNTDGFGLVRDIEVnLGVSLKGARILLLGAGGAARGVVLPMLDRaP 149
                                              *************************************98887666******************9886659 PP

                                TIGR00507 141 keviiaNRtvekaeelaerlqe..lgeilal...sleevelkkvdliinatsaglsgeideaevkaellk 205
                                              ++++i+NRt ekae+l+ ++++      ++l   s   +e  ++d+i+nat+ +l   +  +e +   + 
  lcl|FitnessBrowser__BFirm:BPHYT_RS16975 150 HTLTIVNRTAEKAEALVAQFAQaaRDAACQLsggSARAIEAGTYDVIVNATAGSLDASL--PECDDRAFG 217
                                              ********************99443333444111345678888****************..99******* PP

                                TIGR00507 206 egklvvDlvynpletpllkeakkkgtkvidGlgMlvaQaalsFelwtgvepdvekvfealke 267
                                              +g+l++D++y    t++++ a+k+g++ +dGlgMlv+Qaa sF +w+gv pd   v   l++
  lcl|FitnessBrowser__BFirm:BPHYT_RS16975 218 NGTLAYDMMYGAHPTVFMEHARKLGARGADGLGMLVEQAAESFYVWRGVRPDGAPVLAELRA 279
                                              ***************************************************99888777665 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (287 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 6.49
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis