Alignments for a candidate for ilvD in Burkholderia phytofirmans PsJN

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Burkholderia phytofirmans PsJN

Align dihydroxy-acid dehydratase (EC 4.2.1.9) (characterized)
to candidate BPHYT_RS16735 BPHYT_RS16735 phosphogluconate dehydratase

Query= BRENDA::Q8NQZ9
         (613 letters)



>FitnessBrowser__BFirm:BPHYT_RS16735
          Length = 634

 Score =  225 bits (574), Expect = 4e-63
 Identities = 174/547 (31%), Positives = 263/547 (48%), Gaps = 48/547 (8%)

Query: 34  KPIVAIVNSYTQFVPGHVHLKNVGDIVADAVRKAGGVPKEFNTI-AVDDGIAMGHGGMLY 92
           +P + IV+SY + +  H   KN  DI+  A R+ GGV +    + A+ DG+  G+ GM  
Sbjct: 68  EPNIGIVSSYNEMLSAHAPYKNYPDIIKQAARENGGVAQFAGGVPAMCDGVTQGNAGMEL 127

Query: 93  SLPSREIIADSVEYMVNAHTADAMVCISNCDKITPGMLNAAMRL-NIPVVFVSGGPMEAG 151
           SL SRE+IA S    +  +  DA +C+  CDKI PG+L  A++  +IP +FV  GPM +G
Sbjct: 128 SLFSREVIAMSTAVALTHNMFDAALCLGICDKIVPGLLIGALQFGHIPTIFVPAGPMGSG 187

Query: 152 KAVVVDGVAHAPTDLITAISASASDAVDDAGLAAVEASACPTCGSCSGMFTANSMNCLTE 211
            +           D        A+   D   L   EA+A  + G+C+   TANS   L E
Sbjct: 188 LS---------NDDKAKTRQLFATGQCDRGALLEAEAAAYHSHGTCTFYGTANSNQMLME 238

Query: 212 ALGLSLPGNGSTLATHAARRALFEKAGETVVELCRRYYGEEDESVLPRG-IATKKAFENA 270
            +GL LP +         R AL  +A   V+EL       E  +  P G +  +KA  N 
Sbjct: 239 VMGLHLPSSAFVHPHTPLRDALTAQAARRVLELT-----VERGNYTPIGHVVDEKAIVNG 293

Query: 271 MALDMAMGGSTNTILHILAAAQEGEVDFDLADIDELSKNVPCLSKVAPNSDYHMEDVHRA 330
           +   +A GGSTN  LH++A A+   +  D  D D LS+ VP L+K+ PN    +   H A
Sbjct: 294 IVALLATGGSTNHTLHLVAIARAAGIVIDWDDFDTLSQAVPLLAKIYPNGKADVNHFHAA 353

Query: 331 GGIPALLGELNRGGLLNKDVHSVHSNDLEGWLDDWDIRSGKTTEVATELFHAAPGGIRTT 390
           GG+  L+  L  GGLL++DV++V    L+ + ++  +  GK   V        PG     
Sbjct: 354 GGVAFLVRNLLEGGLLHEDVNTVAGKGLKHYTEEPKLIDGKLQWV--------PGA---- 401

Query: 391 EAFSTENRWDELDTDAAKGCIRDVEHAYTADGGLVVLRGNISPDGAVIKSAGIEEELWNF 450
                     +   D A   +R ++  +  DGGL +++G +     VIK + +  +    
Sbjct: 402 ----------QASEDTA--VLRGIKEPFQPDGGLRLMQGKLG--RGVIKISAVAAQHRKV 447

Query: 451 TGPARVVESQEEAVSVILTKTIQAGEVLVVRYEGPSGGPGMQEMLHPTAFLKG--SGLGK 508
             PA V +SQE          ++   + VVR++G     GM E LH    L G     G 
Sbjct: 448 KAPAIVFDSQEAVQEAFDKGELKRDFIAVVRFQGARAN-GMPE-LHRLTPLLGVLQDQGF 505

Query: 509 KCALITDGRFSGGSSGL-SIGHVSPEAAHGGVIGLIENGDIVSIDVHNRKLEVQVSDEEL 567
             AL+TDGR SG S  + ++ H+SPEA   G IG +  GD++ ID     L++++   E 
Sbjct: 506 HVALVTDGRMSGASGKVPAVIHLSPEALLQGPIGKVRTGDMLVIDAEAGVLDIEIDAAEW 565

Query: 568 QRRRDAM 574
             R +A+
Sbjct: 566 AARPNAV 572


Lambda     K      H
   0.315    0.132    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 901
Number of extensions: 41
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 613
Length of database: 634
Length adjustment: 37
Effective length of query: 576
Effective length of database: 597
Effective search space:   343872
Effective search space used:   343872
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis