Alignments for a candidate for dapL in Burkholderia phytofirmans PsJN

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapL in Burkholderia phytofirmans PsJN

Align N-acetyldiaminopimelate deacetylase; EC 3.5.1.47 (uncharacterized)
to candidate BPHYT_RS27410 BPHYT_RS27410 amidohydrolase

Query= curated2:Q836H7
         (378 letters)



>FitnessBrowser__BFirm:BPHYT_RS27410
          Length = 396

 Score =  196 bits (499), Expect = 7e-55
 Identities = 128/365 (35%), Positives = 184/365 (50%), Gaps = 13/365 (3%)

Query: 12  IRRQLHQIPEIGLEEKETQAFLLNEIDKMKQPYLQVRTWQTGILVFIEGKNPQKTIGWRA 71
           +RR LH  PE+  EE  T   +  E++++     +     TG++  + G NP   I  RA
Sbjct: 21  LRRDLHAHPELRFEEHRTADVVARELEELGYTVSR-GLGGTGVVASLPGANPDWGIVLRA 79

Query: 72  DIDGLPIQEEVVSAFQSKRPGFMHACGHDFHMTIGLGVLKELS-QQQPDNNFLFLFQPAE 130
           D+D LPI E       S   G MHACGHD H  + LG  + L    Q   +  F+FQP E
Sbjct: 80  DMDALPIHEANDFTHASCTHGIMHACGHDGHTVMLLGAARILKGMPQLPGSVHFVFQPGE 139

Query: 131 ENEAGGMLMYEDHAFGEWLPDEFYALHVNPDLPVGTISTRVGTLFAATCEVNITLKGKGG 190
           E  AG   M ++  F  +  +  + +H  P LP G    RVG + AA     IT+ GKG 
Sbjct: 140 EGGAGARKMIDEGLFEHYPTEAVFGMHNWPGLPGGHFGLRVGPIMAAGSRFKITVTGKGA 199

Query: 191 HAAFPHQANDMVLAATNLIQQAQTIVSRNVDPVVGAVVTFGTFHAGTACNVIAEEATLSG 250
           HAA PH   D +  A +++   QTI +R+ DPV  AV++    HAG   NVI + A L G
Sbjct: 200 HAAQPHLGLDPIPLACSMVLHCQTIAARHKDPVDPAVISVCMIHAGDTDNVIPDSAELRG 259

Query: 251 TIRTLTAETNEQTQRRIREISEGIAQSFQCEVTVHLDQKGYLPVVNEPACTTNFIEYMSK 310
           TIRTL++E  ++ QR I+ + EG+A +   +V V   Q  Y P        T   E + +
Sbjct: 260 TIRTLSSELQQKLQRDIQLMCEGLAAAHGAQVEVTFFQ--YYPATINTRAETQLCEAVIR 317

Query: 311 QA---TVQFQQAPVAMTGEDFGYLLSKVPGTMFWL-----GVASPYSLHSAKFEPNEEAL 362
           +           P  MT EDFG++L + PGT   +     G A+P  LH+ K++ N+E +
Sbjct: 318 ETFGDERTHADVPANMTSEDFGFMLEERPGTYVLIGNAHAGTAAP-GLHNPKYDFNDEII 376

Query: 363 LFGVE 367
             GV+
Sbjct: 377 PAGVQ 381


Lambda     K      H
   0.319    0.135    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 447
Number of extensions: 21
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 378
Length of database: 396
Length adjustment: 30
Effective length of query: 348
Effective length of database: 366
Effective search space:   127368
Effective search space used:   127368
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis