Alignments for a candidate for lysT in Burkholderia phytofirmans PsJN

GapMind for Amino acid biosynthesis

Alignments for a candidate for lysT in Burkholderia phytofirmans PsJN

Align Probable methanogen homoaconitase large subunit; HACN; EC 4.2.1.114; Homoaconitate hydratase (uncharacterized)
to candidate BPHYT_RS33915 BPHYT_RS33915 isopropylmalate isomerase

Query= curated2:O27668
         (428 letters)



>FitnessBrowser__BFirm:BPHYT_RS33915
          Length = 469

 Score =  194 bits (492), Expect = 6e-54
 Identities = 143/440 (32%), Positives = 210/440 (47%), Gaps = 60/440 (13%)

Query: 30  VDLAMTHDGTSPPTIRTFRDIASRGGPARVWDPERIVMVFDHNVPANTIGAAEFQRVTR- 88
           +D  + H+ TSP      + +A R     VW     + V DHNVP           V++ 
Sbjct: 27  IDRHLLHEVTSPQAFEGLK-LAERP----VWRISANLAVSDHNVPTTDRSHGIADPVSKL 81

Query: 89  ------EFAREQGIVNIFQN--AAGICHQVLPERGFVRPGMVIVGADSHTCTYGAFGAFA 140
                       GI     N    GI H + PE+G   PGM IV  DSHT T+GAFGA A
Sbjct: 82  QVDTLDSNCDAYGITQFKMNDLRQGIVHIIGPEQGATLPGMTIVCGDSHTSTHGAFGALA 141

Query: 141 TGMGATDMAMVFATGKTWFMVPEAMRIEVTGEPEGHVYAKDVILHIIGEIGVDGATYRSV 200
            G+G +++  V AT        + M ++V G       AKD++L IIG+IG  G T  ++
Sbjct: 142 HGIGTSEVEHVLATQTLLQKKSKNMLVKVEGALPRGCTAKDIVLAIIGKIGTAGGTGYAI 201

Query: 201 EFTGDTIESMDVSGRMTICNMAVEMGAKNGIMEPNRQTLDYVRAR----TGRE------- 249
           EF G TI ++ + GRMT+CNMA+E GA+ G++  +  T++Y++ R     G E       
Sbjct: 202 EFGGSTIRALSMEGRMTVCNMAIEAGARAGMVAVDDTTIEYLKGRPFSPEGVEWDHAVEY 261

Query: 250 FRVYSSDEDSQYLEDHHFDVSDLEPQVA--------------CPDDVDNVYPVHR----- 290
           ++ + +DE + +      + +++ PQV                PD      PV R     
Sbjct: 262 WKQFKTDEGAHFDRVVELNAAEIVPQVTWGTSPEMVTAVDGRVPDPDREKDPVKRDAMER 321

Query: 291 ------------VEGTHIDEAFLGSCTNGRYEDLKIAAEVIG--DRRVHEDVRF-IVSPA 335
                       +E    D+ F+GSCTN R ED++ AA V+    RRV  ++R  +V P 
Sbjct: 322 ALKYMALEPNAPIESIKPDKIFIGSCTNARIEDIRAAAYVVKKLGRRVAPNIRLAMVVPG 381

Query: 336 SREIYLKALEDGIIETFIRAGAIVCNPGCGPCLGAHMGVLAPGEVSIATTNRNFRGRMGD 395
           S  +  +A  +G+ + F  AG     PGC  CL  +   L PGE   +T+NRNF GR G 
Sbjct: 382 SGLVKAQAEREGLDKVFTDAGFEWREPGCSMCLAMNADRLDPGERCASTSNRNFEGRQG- 440

Query: 396 PASSVYLANPAVVAESAIEG 415
                +L +PA+ A +AIEG
Sbjct: 441 AGGRTHLVSPAMAAAAAIEG 460


Lambda     K      H
   0.320    0.137    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 531
Number of extensions: 22
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 428
Length of database: 469
Length adjustment: 33
Effective length of query: 395
Effective length of database: 436
Effective search space:   172220
Effective search space used:   172220
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis