Alignments for a candidate for trpA in Burkholderia phytofirmans PsJN

GapMind for Amino acid biosynthesis

Alignments for a candidate for trpA in Burkholderia phytofirmans PsJN

Align Tryptophan synthase alpha chain; EC 4.2.1.20 (characterized, see rationale)
to candidate BPHYT_RS33865 BPHYT_RS33865 tryptophan synthase alpha chain

Query= uniprot:H0BV16
         (269 letters)



>FitnessBrowser__BFirm:BPHYT_RS33865
          Length = 270

 Score =  307 bits (786), Expect = 2e-88
 Identities = 155/269 (57%), Positives = 200/269 (74%), Gaps = 4/269 (1%)

Query: 1   MSRIASTFSALQAQGRKALIPYVTAGFPFADITPALMHGMVEAGADVIELGVPFSDPMAD 60
           MSRI +TF+AL AQG+K LIP++TAG P    T   MH +   GADVIELGVPFSDPMAD
Sbjct: 1   MSRIKNTFAALSAQGKKGLIPFMTAGDPDPARTVEFMHALAAGGADVIELGVPFSDPMAD 60

Query: 61  GPVIQKAGEKALSLGIGMVQVLDHVREFRKRNNTTPVVLMGYANPVERYDQKHGEGAFVR 120
           GPVIQ++ E+AL+ G+ +  V+  V+ FR+ N+TTPVVLMGYANP+ER     G  AF +
Sbjct: 61  GPVIQQSSERALAHGVSLRHVIADVKRFRETNDTTPVVLMGYANPIERM----GTEAFAK 116

Query: 121 DSAAAGVDGVLIVDYPPEECEAFAASLRAHGMDLIFLLAPTSTDERMAEVARVASGYVYY 180
            +  AGVDGVL+VDYPPEEC  FA  +++ G+D IFLLAPTSTDER+AEV ++ASGYVYY
Sbjct: 117 AAKEAGVDGVLVVDYPPEECANFAEQMQSAGIDPIFLLAPTSTDERIAEVGKIASGYVYY 176

Query: 181 VSLKGVTGSGALDTAAVEQMLPRIRQHVTIPVGVGFGIRDAATAQAIGKVADAVVIGSRI 240
           VSLKGVTG+  LD +++   +P I+  V +PVGVGFGIRDA TA+++ +V+DAVVIGSRI
Sbjct: 177 VSLKGVTGAANLDVSSIASKIPAIKSRVPLPVGVGFGIRDAQTARSVAEVSDAVVIGSRI 236

Query: 241 IQLIEDQEHAKVVPLTIDFLRGIRKALDA 269
           +QL+E             F+  +R+ALD+
Sbjct: 237 VQLLEQAAPEAAAETLTRFVAEVREALDS 265


Lambda     K      H
   0.321    0.137    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 261
Number of extensions: 8
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 269
Length of database: 270
Length adjustment: 25
Effective length of query: 244
Effective length of database: 245
Effective search space:    59780
Effective search space used:    59780
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate BPHYT_RS33865 BPHYT_RS33865 (tryptophan synthase alpha chain)
to HMM TIGR00262 (trpA: tryptophan synthase, alpha subunit (EC 4.2.1.20))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00262.hmm
# target sequence database:        /tmp/gapView.28737.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00262  [M=256]
Accession:   TIGR00262
Description: trpA: tryptophan synthase, alpha subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                -----------
    1.3e-79  252.7   0.2    1.5e-79  252.5   0.2    1.0  1  lcl|FitnessBrowser__BFirm:BPHYT_RS33865  BPHYT_RS33865 tryptophan synthas


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__BFirm:BPHYT_RS33865  BPHYT_RS33865 tryptophan synthase alpha chain
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  252.5   0.2   1.5e-79   1.5e-79       1     255 [.       8     259 ..       8     260 .. 0.98

  Alignments for each domain:
  == domain 1  score: 252.5 bits;  conditional E-value: 1.5e-79
                                TIGR00262   1 fetlkkkeekafvpFvtagdPdleksleiiktlvkaGadalElGvpfsDPlaDGptiqaaelRAlkagvk 70 
                                              f++l ++++k ++pF+tagdPd ++++e+++ l+  Gad++ElGvpfsDP+aDGp+iq+++ RAl++gv+
  lcl|FitnessBrowser__BFirm:BPHYT_RS33865   8 FAALSAQGKKGLIPFMTAGDPDPARTVEFMHALAAGGADVIELGVPFSDPMADGPVIQQSSERALAHGVS 77 
                                              67889999************************************************************** PP

                                TIGR00262  71 vekalellkkvrekasniPivlltyynlifkkgveeFyakakeagvdgvlvaDlPleeaddlleaakkhg 140
                                              ++++++ +k++re++ + P+vl+ y+n+i ++g+e+F + akeagvdgvlv+D P ee +++ e+ ++ g
  lcl|FitnessBrowser__BFirm:BPHYT_RS33865  78 LRHVIADVKRFRETNDTTPVVLMGYANPIERMGTEAFAKAAKEAGVDGVLVVDYPPEECANFAEQMQSAG 147
                                              ********************************************************************** PP

                                TIGR00262 141 vkqiflvaPtaeeerlkkiaekseGfvYlvsvaGvtgarerveeevkelikkvkalskkPvlvGFGiskk 210
                                              +++ifl aPt+ +er+ ++ + ++G+vY vs  Gvtga +   +++ + i  +k+    Pv vGFGi  +
  lcl|FitnessBrowser__BFirm:BPHYT_RS33865 148 IDPIFLLAPTSTDERIAEVGKIASGYVYYVSLKGVTGAANLDVSSIASKIPAIKSRVPLPVGVGFGIRDA 217
                                              ****************************************999999************************ PP

                                TIGR00262 211 eqvkelkelgadgvivGsAlvkiieeklddeekaleeleefvkel 255
                                              + ++++ e+ +d+v++Gs +v+++e+    +e+a e l  fv e+
  lcl|FitnessBrowser__BFirm:BPHYT_RS33865 218 QTARSVAEV-SDAVVIGSRIVQLLEQA--APEAAAETLTRFVAEV 259
                                              *********.889*************9..7899999999999875 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (256 nodes)
Target sequences:                          1  (270 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 10.07
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis