Alignments for a candidate for hcs in Bacteroides thetaiotaomicron VPI-5482

GapMind for Amino acid biosynthesis

Alignments for a candidate for hcs in Bacteroides thetaiotaomicron VPI-5482

Align homocitrate synthase (EC 2.3.3.14) (characterized)
to candidate 351389 BT1861 2-isopropylmalate synthase (NCBI ptt file)

Query= BRENDA::D0VY45
         (540 letters)



>FitnessBrowser__Btheta:351389
          Length = 498

 Score =  395 bits (1015), Expect = e-114
 Identities = 228/509 (44%), Positives = 318/509 (62%), Gaps = 22/509 (4%)

Query: 27  ILDTTLRDGEQSPGAAMTCVQKLETARQLAKLGVDIIEAGFPCASKQDFMAVKMIAEEVG 86
           I DTTLRDGEQ PG  +  V+K++ A+ L  LGVD+IEAGFP +S  DF +V  I++ V 
Sbjct: 7   IFDTTLRDGEQVPGCQLNTVEKIQVAKALEALGVDVIEAGFPISSPGDFNSVIEISKAVT 66

Query: 87  NCVDGNGYVPVITGVSRCNEKDIATAWEALKHAKRPRLRTFIATSPIHMEYKLRKSKDQV 146
                    P I  ++R  +KDI  A +ALK AK  R+ T I TS  H++YK   +++++
Sbjct: 67  --------WPTICALTRAVQKDIDVAVDALKFAKHKRIHTGIGTSDSHIKYKFNSNREEI 118

Query: 147 LETARNMVKFARSLGCTDIQFGAEDAARSDKEFLYQIFGEVIKAGATTLTIPDTVGIAMP 206
           +E A   VK+AR     D++F AEDA R+D E+L ++   VIKAGAT + IPDT G  +P
Sbjct: 119 IERAVAAVKYARRF-VDDVEFYAEDAGRTDNEYLARVVEAVIKAGATVVNIPDTTGYCLP 177

Query: 207 FEYGKLIADIKANTPGIENAIMATHCHNDLGLATANTIEGARYGARQLEVTINGIGERAG 266
            EYG  I  +  +  GI+NAI++THCHNDLG+ATANTI G   GARQ+EVTINGIGERAG
Sbjct: 178 SEYGAKIKYLIDHVDGIDNAILSTHCHNDLGMATANTIAGVLNGARQVEVTINGIGERAG 237

Query: 267 NASFEEVVMAL-TCRGIDILGGLHTGINTRHILKTSKMVEKYSGLHLQPHKALVGANAFL 325
           N + EE+ M + +   IDI     T INT+ I  TS+MV     + +QP+KA+VG NAF 
Sbjct: 238 NTALEEIAMIIKSHHEIDI----QTNINTQKIYPTSRMVSSLMNMPVQPNKAIVGRNAFA 293

Query: 326 HESGIHQDGMLKHRGTYEIISPEDIGLVRSVGDTIVLGKLSGRQALRNRLEELGYKLKDT 385
           H SGIHQDG+LK+  TYEII P D+G+     ++IVL   SGR AL+NRL  LG  L   
Sbjct: 294 HSSGIHQDGVLKNVETYEIIDPHDVGI---DDNSIVLTARSGRAALKNRLSLLGVNLDQE 350

Query: 386 EVEGVFWQFKAVAEKKKRITDTDLRALVSNEAFNEQPIWKLGDLQVTCGTVGFSTATVKL 445
           +++ V+ +F  +A+KKK I D D+  L   +      I KL  LQVT G    S A++ L
Sbjct: 351 KLDKVYEEFLKLADKKKDINDDDVLVLAGADRSQNHRI-KLEYLQVTSGVGVRSVASLGL 409

Query: 446 FSIDGSMHVACSIGTGPVDSAYKAINHIVKEPAKLVKYTLGAITEGIDATATTSVEISRG 505
            +I G    AC+ G GPVD+A KA+  IV     L ++T+ AI++G D      +++   
Sbjct: 410 -NISGEKFEACASGNGPVDAAIKALKKIVDRHMTLKEFTIQAISKGSDDVGKVHMQV--- 465

Query: 506 DTNHPVFSGTGGGTDVVVSSVDAYLSALN 534
           + ++ ++ G G  TD++ +SV+AY+  +N
Sbjct: 466 EYDNQIYYGFGANTDIIAASVEAYIDCIN 494


Lambda     K      H
   0.318    0.134    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 641
Number of extensions: 30
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 540
Length of database: 498
Length adjustment: 35
Effective length of query: 505
Effective length of database: 463
Effective search space:   233815
Effective search space used:   233815
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis