Alignments for a candidate for PPYAT in Bacteroides thetaiotaomicron VPI-5482

GapMind for Amino acid biosynthesis

Alignments for a candidate for PPYAT in Bacteroides thetaiotaomicron VPI-5482

Align Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase; Aspartate 4-decarboxylase; Asd; AsdP; EC 2.6.1.1; EC 4.1.1.12 (characterized)
to candidate 350263 BT0735 aspartate decarboxylase AsdA (NCBI ptt file)

Query= SwissProt::Q53IZ1
         (531 letters)



>FitnessBrowser__Btheta:350263
          Length = 557

 Score =  463 bits (1192), Expect = e-135
 Identities = 240/526 (45%), Positives = 338/526 (64%), Gaps = 9/526 (1%)

Query: 6   RSLANLSPFELKDELIKVASGKANRL---MLNAGRGNPNFLATTPRRAFFRLGLFAAAES 62
           + +  +SPFELK++LI++A     ++   MLNAGRGNPN++AT PR AFF LG F   E 
Sbjct: 31  KKMETISPFELKNKLIEMADESIKKMAHTMLNAGRGNPNWIATEPREAFFLLGKFGLCEC 90

Query: 63  ELSYSYMTVGVGGLAKLDGIEGRFERFIAEHRDQEGVKFLGKSLSYVRDQLGLDPAAFLH 122
               S +  G+ G+ + +GI  RFE F+ E+  + G + L ++ +Y+  +   DP   +H
Sbjct: 91  RRVQS-LEEGIAGIPQQEGIAARFEAFLKENEKEAGARLLKETYNYMLMEHAADPDRLVH 149

Query: 123 EMVDGILGCNYPVPPRMLTVSEQIVRQYIVREMAGGAVPPESVDLFAVEGGTAAMAYIFE 182
           E  + ++G  YPVP R+L  +E IV+ Y+ +EM     P  + DLFA EGGTAAM Y+F+
Sbjct: 150 EWAESVIGDQYPVPDRILHFTELIVQDYLAQEMCDRRPPKGTFDLFATEGGTAAMCYVFD 209

Query: 183 SLRISGLLKAGDKVAIGMPVFTPYIEIPELAQYDLKEVPIHAD---PD--NGWQYSDAEL 237
           SL+ + LL  GD +A+ +PVFTPYIEIPEL +Y      I AD   PD  + WQY D ++
Sbjct: 210 SLQENFLLNQGDSIALMIPVFTPYIEIPELRRYQFDVTEISADQMTPDGLHTWQYKDEDI 269

Query: 238 DKLKDPDVKIFFCVNPSNPPSVKMDQRSLDRVRAIVAEQRPDLLILTDDVYGTFADEFQS 297
           DKLK+P +K  F  NPSNPPS  +   +  R+  IV    P+L+I+TDDVYGTF   F+S
Sbjct: 270 DKLKNPQIKALFITNPSNPPSYALSPETAARIVNIVKNDNPNLMIITDDVYGTFIPHFRS 329

Query: 298 LFSVCPRNTLLVYSFSKYFGATGWRLGVIAAHKDNVFDHALSQLPESAKKALDHRYRSLL 357
           L +  P NTL VYSFSKYFGATGWR  VIA H+DN++D  +++L E     L+ RY SL 
Sbjct: 330 LMAELPHNTLCVYSFSKYFGATGWRNAVIALHEDNIYDKMIARLSEEQTAILNKRYASLS 389

Query: 358 PDVRSLKFIDRLVADSRVVALNHTAGLSTPQQVQMVLFSLFALMDEADAYKQALKQLIRR 417
                +KFIDR+VADSR +ALNHTAGLS PQQ+QM LF+ F+L+D+ D YK  ++++I R
Sbjct: 390 LHPEKMKFIDRMVADSRQIALNHTAGLSLPQQMQMSLFAAFSLLDKEDRYKAKMQEIIHR 449

Query: 418 REATLYRELGMPPLENPNSVNYYTLIDLQNVTCRLYGEAFSQWAVQQSSTGDMLFRVADE 477
           R   L+   G   +E+P    YY+ ID+     + YG+ F+ +  +  +  D++FR+A+E
Sbjct: 450 RLHALWDSTGFTLIEDPLRAGYYSEIDMLVWAKKFYGDEFADYLQKTYNPLDVVFRLANE 509

Query: 478 TGIVLLPGRGFGSDRPSGRASLANLNEYEYAAIGRALRRLADELYE 523
           T +VLL G GF   + S R SLANLNE +Y  IG++++R+ DE  E
Sbjct: 510 TSLVLLNGGGFAGPKWSVRVSLANLNEADYVKIGQSIKRVLDEYAE 555


Lambda     K      H
   0.321    0.138    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 720
Number of extensions: 27
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 531
Length of database: 557
Length adjustment: 35
Effective length of query: 496
Effective length of database: 522
Effective search space:   258912
Effective search space used:   258912
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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Rules (tab-delimited)
Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis