GapMind for Amino acid biosynthesis

 

Aligments for a candidate for cmutase in Bacteroides thetaiotaomicron VPI-5482

Align candidate 353460 BT3934 (putative phospho-2-dehydro-3-deoxyheptonate aldolase/chorismate mutase (NCBI ptt file))
to HMM PF01817 (CM_2)

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/PF01817.21.hmm
# target sequence database:        /tmp/gapView.11167.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       CM_2  [M=75]
Accession:   PF01817.21
Description: Chorismate mutase type II
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                          Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                          -----------
    6.8e-24   70.4   1.3    6.8e-24   70.4   1.3    1.8  2  lcl|FitnessBrowser__Btheta:353460  BT3934 putative phospho-2-dehydr


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Btheta:353460  BT3934 putative phospho-2-dehydro-3-deoxyheptonate aldolase/chorismate mutase (NCB
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ?   -3.2   0.1      0.65      0.65      40      52 ..      26      38 ..      24      48 .. 0.62
   2 !   70.4   1.3   6.8e-24   6.8e-24       1      74 [.     270     347 ..     270     348 .. 0.96

  Alignments for each domain:
  == domain 1  score: -3.2 bits;  conditional E-value: 0.65
                                       HHHHHHHHHHHHH CS
                               CM_2 40 eReaevlerleeg 52
                                       e e++v+e  ++ 
  lcl|FitnessBrowser__Btheta:353460 26 ETEEQVMETAKQL 38
                                       5677888777763 PP

  == domain 2  score: 70.4 bits;  conditional E-value: 6.8e-24
                                        HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCTSGSS-HHHHHHHHHHHHH.HH...S-HHHHHHHHHHHHHHHHH CS
                               CM_2   1 RkeIdeiDdeileLLaeRaelakevgeyKkeeglpvydpeReaevlerlee.ga...ldeeaierifreiisasra 72 
                                        Rk+Ide+Dd i++ La+R+++a+e+g yKke+g +v++ +R +e+le++ + g+   +++++++ if++i+++s++
  lcl|FitnessBrowser__Btheta:353460 270 RKQIDECDDNIIQELAKRMRVAREIGTYKKEHGITVLQAGRYNEILEKRGAqGEqcgMSADFMKLIFEAIHEESVR 345
                                        9**************************************************733689******************9 PP

                                        HH CS
                               CM_2  73 lq 74 
                                        +q
  lcl|FitnessBrowser__Btheta:353460 346 QQ 347
                                        98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (75 nodes)
Target sequences:                          1  (353 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 8.57
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory